purE (P. aeruginosa ) in pDONR201 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  purE
Gene Name:  phosphoribosylaminoimidazole carboxylase catalytic subunit
Original Clone ID:
FLH045360.01F
Keyword:
None
Species:
Pseudomonas aeruginosa
Type:
cDNA
Vector Name:
pDONR201
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
PaCD00008343
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 492nts
Open reading frame: 1 to 492

ATGAGCGCACTGGTTGGCGTGATCATGGGTTCCAAATCGGACTGGAGCACCCTCAGTCAC
ACCGCGGACATGCTGGACAAGCTGGGCATCCCCTACGAAGTGAAGGTGGTTTCCGCCCAC
CGCACCCCGGACCTGCTCTTCCAGTACGCCGAGGAAGCCGAAGGCCGCGGCCTCGAAGTG
ATCATCGCCGGCGCTGGCGGCGCCGCCCACCTGCCGGGCATGTGCGCGGCCAAGACCCAC
CTGCCGGTACTCGGCGTGCCGGTACAGTCGTCGATGCTGTCGGGTGTCGACTCGCTGCTC
TCCATCGTGCAGATGCCCGCCGGCGTACTGGTCGCCACCCTGGCCATCGGCAAGGCCGGC
GCGGTCAACGCGGCGCTGCTCTCGGCGAGCATCCTCGGCGCCAAGCACCCGCAGTACCAC
GAGGCGCTGAAACAGTTCCGCGCCAACCAGACCGAAACCGTGCTGGACAATCCCGACCCG
CGCGATGCGGGA

Protein sequence predicted based on nucleotide sequence

MSALVGVIMGSKSDWSTLSHTADMLDKLGIPYEVKVVSAHRTPDLLFQYAEEAEGRGLEV
IIAGAGGAAHLPGMCAAKTHLPVLGVPVQSSMLSGVDSLLSIVQMPAGVLVATLAIGKAG
AVNAALLSASILGAKHPQYHEALKQFRANQTETVLDNPDPRDAG

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 492
Mutations:
None
Discrepancy:
c329t,P110L; g432a
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable