Gene:
Gene Symbol:
Itga7
Gene Name:
integrin alpha 7
Original Clone ID:
FLH270562.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083486
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3408nts
Open reading frame: 1 to 3408
ATGGCCAGGATTCCGAGGTGCGATTTTCTCCGGCCCCCCGGAATCTACTATCTTATTACT
TCCTTGCTCGCTGGACTGTTCTTACCACCGGCTATCGCCTTCAATCTGGATGTGATGGGT
GCCATACGCAAGGAGGGAGAACCCGGCAGCCTATTCGGCTTTTCGGTGGCCCTGCACCGA
CAATTACAGCCCCGACCCCAGAGCTGGCTGCTGGTGGGCGCTCCCCAGGCCCTGGCTCTT
CCTGGACAGCAGGCAAACCGCACCGGAGGCCTCTTTGCTTGTCCCCTGAGCCTAGAGGAG
ACAGATTGCTACAGAGTGGACATTGACAGGGGAGCTAACGTGCAGAAGGAGAGCAAGGAG
AACCAATGGCTGGGAGTCAGTGTCCGGAGCCAGGGGCCAGGGGGCAAGATTGTTACGTGT
GCACACCGATATGAGTCTCGACAGAGAGTGGACCAGGCTTTGGAGACTCGGGATGTGATT
GGCCGCTGCTTTGTGCTAAGCCAGGACCTGGCCATCCGTGATGAGCTGGATGGTGGGGAG
TGGAAGTTCTGTGAGGGGCGCCCCCAGGGCCATGAACAGTTTGGGTTCTGTCAGCAGGGC
ACAGCTGCCACCTTCTCCCCTGACAGTCACTACCTCGTCTTTGGGGCTCCAGGAACCTAT
AACTGGAAGGGGTTGCTTTTTGTGACCAACATTGATAGCTCAGACCCTGACCAGCTGGTG
TATAAAACTTTGGACCCTGCTGACCGGCTCACAGGACCAGCCGGAGACTTGACCTTGAAT
AGCTATTTAGGTTTCTCCATCGATTCTGGGAAGGGTCTTATGCGCTCAGAAGAGCTGAGT
TTTGTGGCAGGGGCCCCACGTGCCAACCACAAAGGGGCTGTGGTCATTCTGCGCAAGGAT
AGCGCCAGCCGCTTGATACCTGAGGTTGTGCTGTCTGGGGAGCGCCTGACCTCTGGCTTT
GGCTACTCACTGGCTGTGACTGATCTCAACAATGATGGCTGGGCAGACCTGATTGTGGGT
GCCCCCTACTTCTTTGAACGCCAAGAAGAGCTGGGAGGTGCTGTGTATGTGTACATGAAC
CAGGGTGGCCATTGGGCAGATATCTCTCCTCTCCGAATCTGTGGCTCCCCTGATTCCATG
TTTGGGATCAGTTTGGCTGTATTGGGGGACCTCAACCAAGATGGCTTCCCAGACATTGCC
GTGGGAGCTCCCTTTGACGGAGATGGGAAGGTCTTTATCTACCATGGGAGCAGCCTTGGG
GTGGTTGTCAAACCTTCACAGGTGCTGGAGGGCGAGGCGGTGGGTATCAAGAGCTTTGGT
TACTCCCTGTCTGGTGGCCTGGATGTGGATGGAAATCACTACCCAGACCTGCTTGTGGGC
TCCCTGGCTGATACCGCTGCTCTGTTCAGGGCCAGACCTGTTCTACATGTCTCCCAAGAG
ATCTTCATTGACCCCAGAGCCATCGATCTGGAACAACCCAACTGTGCCGATGGACGCTTG
GTCTGTGTGGACATAAAGATCTGTTTCAGCTATGTCGCTGTGCCCAGCAGCTACAGCCCT
AGTGTGGCCTTAGATTATATGTTAGATGGGGACACAGACCGGAGGCTCCGGGGCCAGGTT
CCACGTGTGACGTTTCTGAGCCGAGGCCTGGATGACCTCAGGCATCAGTCCTCCGGCACC
GTGTGGCTGAAGCACCAACATGACCGAGTCTGTGGAGATACTGTGTTCCAGCTGCAGGAA
AACGTCAAAGACAAGCTACGGGCCATTGTGGTGACCCTGTCATATGGTCTCCGAACCCCT
CCATTAGGGAGGCAAGCACCTGGCCAGGAGCTCCCCACTGTGGCTCCCATCCTCAATGCT
CACCAGCCCAGCACCCAGAGGACTGAGATCCACTTTCTGAAGCAAGGCTGCGGTGAAGAT
AAGATCTGTCAGAGCAACCTCCAGCTCGAGCGGTACCAGTTCTGTTCCAGGATCAGCGAC
ACAGAGTTCCAGGCTCTGCCCATGGATCTGGATGGAAGGACCGCCCTGTTCGCATTGAGT
GGGCAGCCGTTCATAGGCCTGGAACTGACAGTCACCAACCTGCCCTCTGACCCTGCCCGG
CCTCAGGCAGATGGGGATGATGCTCATGAAGCACAGCTCTTGGTCACTCTCCCAGCCTCT
CTACGGTACTCAGGAGTCCGTGCTCTGGACTCTGTGGAGAAGCCGCTCTGCCTGTCCAAT
GACAGCGCCTCTCATGTCGAGTGTGAGCTGGGGAACCCTATGAAGAGAGGCGCTCAGGTC
ACTTTTTACCTCATCCTCAGCACCTCTGGGATCACTATTGAGACCACAGAGCTGGAGGTG
AAATTGCTGTTAGCCACGATCAGTGAGCAGGAGCTGGATCCGGTCTCCGTTCGGGCTCAT
GTCTTCATTGAATTGCCACTGTCCATTTCAGGGGTGGCCACTCCCCAGCAACTCTTCTTC
TCTGGCGAGGTGAAGGGCGAAAGTGCCATGCGATCTGAGAGGGACGTGGGCAGCAAAGTC
AAGTATGAGGTCACGGTCTCCAATCAAGGCCAGTCTCTCAATACTCTGGGCTCTGCGTTC
CTCAACATCATGTGGCCCCACGAGATCGCCAATGGAAAGTGGCTGCTGTACCCCATGCGG
GTAGAGCTGGAGGGCGGACAGGGGCCTGGCAAGAGAGGGATCTGTTCCCCAAGACCCAAC
ATCCTCCAGCTGGATGTGGACAGCAGGGATAGGAGGCGGCGAGAGCTGGGGCAGCCGGAG
CCGCAGGAGCCTCCAGAGAAGGTGGAGCCTAGCACATCCTGGTGGCCAGTGTCCTCTGCT
GAGAAGAGAAACGTGACTCTGGACTGCGCCCAGGGCACGGCCAAGTGTGTGGTGTTCAGC
TGCCCACTCTACAGCTTTGATCGTGCAGCCGTGCTTCACGTCTGGGGCCGCCTCTGGAAC
AGCACCTTTCTGGAGGAGTACATGGCCGTGAAATCCCTGGAAGTGATCGTCCGAGCCAAC
ATCACAGTGAAATCCTCCATTAAGAACTTGTTGCTCAGAGATGCATCCACAGTGATTCCA
GTGATGGTGTACTTGGACCCCATGGCCGTGGTTGTGGAAGGAGTCCCCTGGTGGGTCATC
CTCCTGGCAGTGCTGGCTGGGCTGTTAGTCCTGGCCTTGCTGGTACTGCTGCTGTGGAAG
CTGGGATTCTTCAAGCGGGCGAAGCACCCCGAGGCCACCGTGCCCCAGTACCACGCAGTG
AAGATCCCTCGGGAAGACCGACAGCAGTTCAAGGAGGAGAAGACAGGCACCATCCAGAGG
AGTAACTGGGGCAACTCCCAGTGGGAGGGCTCCGACGCACACCCCATCTTGGCTGCCGAC
TGGCATCCTGAGCTGGGTCCTGATGGACATCCGGTGCCAGCCACTGCC
Protein sequence predicted based on nucleotide sequence
MARIPRCDFLRPPGIYYLITSLLAGLFLPPAIAFNLDVMGAIRKEGEPGSLFGFSVALHR QLQPRPQSWLLVGAPQALALPGQQANRTGGLFACPLSLEETDCYRVDIDRGANVQKESKE NQWLGVSVRSQGPGGKIVTCAHRYESRQRVDQALETRDVIGRCFVLSQDLAIRDELDGGE WKFCEGRPQGHEQFGFCQQGTAATFSPDSHYLVFGAPGTYNWKGLLFVTNIDSSDPDQLV YKTLDPADRLTGPAGDLTLNSYLGFSIDSGKGLMRSEELSFVAGAPRANHKGAVVILRKD SASRLIPEVVLSGERLTSGFGYSLAVTDLNNDGWADLIVGAPYFFERQEELGGAVYVYMN QGGHWADISPLRICGSPDSMFGISLAVLGDLNQDGFPDIAVGAPFDGDGKVFIYHGSSLG VVVKPSQVLEGEAVGIKSFGYSLSGGLDVDGNHYPDLLVGSLADTAALFRARPVLHVSQE IFIDPRAIDLEQPNCADGRLVCVDIKICFSYVAVPSSYSPSVALDYMLDGDTDRRLRGQV PRVTFLSRGLDDLRHQSSGTVWLKHQHDRVCGDTVFQLQENVKDKLRAIVVTLSYGLRTP PLGRQAPGQELPTVAPILNAHQPSTQRTEIHFLKQGCGEDKICQSNLQLERYQFCSRISD TEFQALPMDLDGRTALFALSGQPFIGLELTVTNLPSDPARPQADGDDAHEAQLLVTLPAS LRYSGVRALDSVEKPLCLSNDSASHVECELGNPMKRGAQVTFYLILSTSGITIETTELEV KLLLATISEQELDPVSVRAHVFIELPLSISGVATPQQLFFSGEVKGESAMRSERDVGSKV KYEVTVSNQGQSLNTLGSAFLNIMWPHEIANGKWLLYPMRVELEGGQGPGKRGICSPRPN ILQLDVDSRDRRRRELGQPEPQEPPEKVEPSTSWWPVSSAEKRNVTLDCAQGTAKCVVFS CPLYSFDRAAVLHVWGRLWNSTFLEEYMAVKSLEVIVRANITVKSSIKNLLLRDASTVIP VMVYLDPMAVVVEGVPWWVILLAVLAGLLVLALLVLLLWKLGFFKRAKHPEATVPQYHAV KIPREDRQQFKEEKTGTIQRSNWGNSQWEGSDAHPILAADWHPELGPDGHPVPATA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
177
End on reference sequence
3587
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
