Gene:
Gene Symbol:
Rasal3
Gene Name:
RAS protein activator like 3
Original Clone ID:
FLH270595.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083519
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3123nts
Open reading frame: 1 to 3123
ATGAAGCCAGAATGTGGGCAGACAATGTTCAGAACCTTTTGGTCTCGCAGCAGAGACTCC
TCCGCCATGGACCCACCGTTGCAGAGTGAAGAAGATTCCCAAACGCAGCCCTCGCTGCCC
TCTCCGCTGACATCCTACCGCTGGCACACTGGAGGCAGTGGGGAAAAGGCTGCTGGAGGG
TTCCGCTGGGGCCGCTTTGCAGGCTGGGGAAGGGCCCTGAGCCATCAGGAGCCCATGGTG
AACAGCCAGCCAGCCCCCAGATCTCTGTTTCGTCGGGTCCTCTCTGCACCCCCTAAGGAG
TCACGGTCCAATCGCCTCCGGTTTTCCAAGACCCTGTGGGGAAGGCACAAGAACGTGGCA
CCACTGGAACCCAAGCCAAATCCGAAGGCCCCAGAGCCAGAGCTGGAGTTGGTAGCAGAC
CCAGATCTACCCGTTGCACAGATCCCTGAGCCTCCCACCCCGGACATGCCTGTTTGGAAC
ATTGATGGCTTTACCCTTCTCGAAGGAAAGCTGGTGATGCTCGGTGAGGAGGAGGGTCCT
CGCCAAATCCGAGTGGGAAGCGCCAGTTCAGAGAACAGCATGCAGGCAGCTTTGGGGAAC
CTCAAGGATGCAGTTCGGACCCCTGGAAAAACTGAGCCGGAAGCTGCTGGTTCCAACCAG
GTCCACAATGTTCGGAAGTTACTCAAGAGGCTGAAGGAGAAGAAAAGAGCCAAGTCAGAA
CTGGGAGCCTACACCCCTCGAGATGGACCTCCCAGTGCCCTCGGTTCCAGAGAATCGCTG
GCCACACTCTCTGAGCTGGACCTAGGTGCTGAGAGGGACGTGAGAGTCTGGCCACTGCAT
CCTAGCCTCTTGGGGGAGCCCTACTGCTTCCAGGTAACCTGGGCAGGTGGGAGCCTCTGT
TTCTCATGTCGCTCGAGCGCTGAGAGAGACCGTTGGATTGAGGACCTTCGTCGGCAGTTC
CAGCCGAGCCAGGACAACGTGGAGCGGCAAGAGATGTGGCTGACGGTGTGGGTGCACGAG
GCCAAAGGGCTACCCCGAGCAACTGTACCCGGAGTGCGTGCGGAGCTGTGGTTGGATGGC
GCGCTATTGGCGCGCACTGCCCCCCGTGCCGGCCCAGGCCAACTCTTCTGGGCTGAACGC
TTCCACTTCGAGGCGTTGCCACCTGCCCGTCGCCTGTCGCTGCGGTTGCGCAGCGCAGGC
CCGGCAGGGGCAACCGTGGGCAGGGTGGTGCTGGAGCTGGACGAGGTGAGCATCCCCCGC
GCGCCTGCCGCGGGCCTAGAGCGCTGGTTCCCGGTGCTTGGGGCGCCGGCAGGTGCGGTG
CTACGAGCGCGGATCAGGGTGCGTTGTCTGCGCGTGCTGCCGTCGGAGCGCTATAAGGAG
CTGGCCGAGTTCCTCACCTTCCACTACGCGCGCCTGTGCGGGGCGCTGGAGCCGGCGCTG
TCTGCTCAGGCCAAAGAGGAGCTGGCAGCAGCCATGGTGCGCGTGCTACGAGCCACTGGC
CGAGCGCAGGCATTGGTGACAGACCTAGGAACCGCAGAGCTGGCACGCTGTGGAGGCCGT
GAAGCACTGCTATTCCGAGAAAATACCTTAGCCACAAAGGCCATTGATGAATACATGAAA
CTGGTGGCACAGGAGTACCTCCAGGACACCCTGGGGCAGGTTGTGCGGTGTCTCTGTGCC
TCCACTGAGGACTGTGAAGTGGACCCCAGCAAGTGCCCAACACCAGAGCTGCCAAAACAC
CAAGCTAGACTGCGAGACAGCTGTGAGGAGGTTTTTGAAAACATCATCCATTCTTACAAC
TGTTTCCCAGCAGAGCTGGGCTCCGTGTTCTCAAGTTGGCGTGAAGCATGCAAAGCACGA
GGCTCTGAGGCCCTGGGCCCCCGGCTGGTGTGTGCTTCCCTCTTCCTGAGGCTTTTATGC
CCTGCCATCTTGGCACCTAGCCTCTTTGGCCTGGCACCAGAGCACCCAGCCCCAGGCCCA
GCCAGAACTCTTACACTCATCGCCAAGGTCATCCAGAACCTCGCCAACTGTGCCCCGTTT
GGTGAGAAGGAAGCCTACATGGCGTTTATGAATAGCTTCCTGGAAGATCATGGACCAGCC
ATGCAACACTTCCTGGACCAGGTAGCTACGGTGGATGCAGATACCACACCCAGTGGCTAC
CAGGGAAGTGGAGACCTGGCCCTTCAGTTAGCAGTTCTTCATGTCCAGCTCTGCACAATC
TTTGCCGAACTTGACCAGAAAACCCAAGATAGCTTGGAACCACTACCCACCATCCTTCGA
GCCATTGAGGAAGGCCGGCCTGTCCCAGTGTCTGTACCAATGCGTCTTCCCCGGATCTCC
ACTCAGGTCCAATCCAGCTTCTTTTCAGGGGAGAAACCCGGCTTCCTGGCACCCCGAGAC
CTCCCCAAGCACACTCCCCTCATTTCCAAGAGTCAATCTCTGCGCAGCTTTCAAGGGGCA
GGAAGCTGGGCCAGTCGGCGGCCAGATGAGGAGCGGCCCCAGAGGCGGCCGCGGCCAGTG
CTGCGCACACAGAGCGTCCCTGCCAGACGTCCTACCCACCGTCGCCCTTCTGCAGGTTCC
AAGCCGCGGCCCAAAGGCTCCCTACGCATGGGCCCCGCGCCTTGCGGACGGGCCTGGACT
AGGGCTTCTGCCTCGCTACCTCGGAAGCCATCGGTACCATGGCAGCGCCAAATGGACCAG
CCGGGGGACCGATACCAAACTACGGGAACGCACCGACCAGTGGGCAAGCTAGCAGAGATA
CAATGTGAGGTGGCTATATTCCGCGAGGCACAGAAAGCTCTGTCCCTTCTGGTGGAGTCG
TTGAGTACCCAAGTCCAAGCCTTGAAGGAGCAGCAGGAGCACTTCCGCTGTCAGCTGCAG
GATCTGTACTCCAGACTGGGAGCTGGTATCTCAAAGTTGGATTCTAAGGGTGGTCTCCCA
AGCAACGGAAGCCACAGGCTGAAAAGTCTGGAACAGCGCCTGACTGAGATGGAATGTTCT
CAGGACCAGCTGAGGGATAGCCTCCAAAGCCTGCAACTTCTTTCAAAAACACCAGGATCT
CGGAGCCAGCCCCTGCCTCTCAAAGCACCATGTGTCAATGGAGCTGATCTGTCAATGGGC
ACT
Protein sequence predicted based on nucleotide sequence
MKPECGQTMFRTFWSRSRDSSAMDPPLQSEEDSQTQPSLPSPLTSYRWHTGGSGEKAAGG FRWGRFAGWGRALSHQEPMVNSQPAPRSLFRRVLSAPPKESRSNRLRFSKTLWGRHKNVA PLEPKPNPKAPEPELELVADPDLPVAQIPEPPTPDMPVWNIDGFTLLEGKLVMLGEEEGP RQIRVGSASSENSMQAALGNLKDAVRTPGKTEPEAAGSNQVHNVRKLLKRLKEKKRAKSE LGAYTPRDGPPSALGSRESLATLSELDLGAERDVRVWPLHPSLLGEPYCFQVTWAGGSLC FSCRSSAERDRWIEDLRRQFQPSQDNVERQEMWLTVWVHEAKGLPRATVPGVRAELWLDG ALLARTAPRAGPGQLFWAERFHFEALPPARRLSLRLRSAGPAGATVGRVVLELDEVSIPR APAAGLERWFPVLGAPAGAVLRARIRVRCLRVLPSERYKELAEFLTFHYARLCGALEPAL SAQAKEELAAAMVRVLRATGRAQALVTDLGTAELARCGGREALLFRENTLATKAIDEYMK LVAQEYLQDTLGQVVRCLCASTEDCEVDPSKCPTPELPKHQARLRDSCEEVFENIIHSYN CFPAELGSVFSSWREACKARGSEALGPRLVCASLFLRLLCPAILAPSLFGLAPEHPAPGP ARTLTLIAKVIQNLANCAPFGEKEAYMAFMNSFLEDHGPAMQHFLDQVATVDADTTPSGY QGSGDLALQLAVLHVQLCTIFAELDQKTQDSLEPLPTILRAIEEGRPVPVSVPMRLPRIS TQVQSSFFSGEKPGFLAPRDLPKHTPLISKSQSLRSFQGAGSWASRRPDEERPQRRPRPV LRTQSVPARRPTHRRPSAGSKPRPKGSLRMGPAPCGRAWTRASASLPRKPSVPWQRQMDQ PGDRYQTTGTHRPVGKLAEIQCEVAIFREAQKALSLLVESLSTQVQALKEQQEHFRCQLQ DLYSRLGAGISKLDSKGGLPSNGSHRLKSLEQRLTEMECSQDQLRDSLQSLQLLSKTPGS RSQPLPLKAPCVNGADLSMGT
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
133
End on reference sequence
3258
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
