Gene:
Gene Symbol:
Grin3b
Gene Name:
glutamate receptor, ionotropic, NMDA3B
Original Clone ID:
FLH270596.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083520
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3009nts
Open reading frame: 1 to 3009
ATGGAGTGTGTGCAGACGCTGTGGCTCAGCCTGGCCCTGGCGCTGGCGCGAGGGTCCTGG
GTGGTGCGCGGTCACCCTCAGCCCTGCGGGGTTCCCACGCGCGCCGGGGCCTCCGTGCGC
CTGGCTGCGCTCCTACCCCGGGCGCCCGCCGCCCGCGCCCGCGTCCTAGCCGCCCTGGCC
ACCCCTTCCCCGCGGCTGCCGCACAACCTGAGTCTGGAGCTAGTGGCCGTCGCGTCCCCA
ACCCGGGACCCCGCGTCGCTGGCCCGAGGTCTGTGCCAGGTTCTGGCACCGCCCGGCGTG
GTGGCCTCTATAACCTTTCCCGAGGCGCGGCCTGAGCTACGGCTATTGCAGTTCCTGGCA
GCTGCCACAGAGACCCCGGTGCTGAGCGTCCTACGGAGGGAGGTGCGCGCGCCCCTCGGA
GCTCCGACCCCGTTCCACCTGCAGCTGGACTGGGCTAGTCCCCTGGAGACCATCCTGGAT
GTGCTGGTGTCCCTGGTACGGGCACACGCCTGGGAGGACATTGCTCTAGTGCTCTGTCGT
GTCCGGGACCCCAGTGGCCTGGTGACACTCTGGACCAGCCGTGCTAGCCAGGCTCCAAAG
TTTGTGCTGGACCTGAGCCAGTTGGACAGCGGGAATGACAGCCTTCGGGCTACACTGGCC
CTGCTGGGGACGCTGGAAGGAGGGGGAACCCCCGTGTCTGCAGCCGTCCTCCTGGGCTGC
AGCACTGCCCATGCACATGAGGTCCTAGAGGCAGCACCACCGGGTCCCCAGTGGCTGCTG
GGCACACCACTGCCCGCCGAGGCACTGCCCAAAACCGGTCTGCCCCCTGGGGTGTTGGTG
CTGGGGGAAACCGGGCAGCCTTCCCTGGAAGCTGCCGTCCACGACATGGTGGAGCTTGTG
GCTCGGGCACTCAGCAGCATGGCCCTCATGCACCCAGAGCGGGCCCTGCTTCCAGCGGCA
GTAAATTGTGAGGACCTGAAAACGGGCGGCTCTGAGTCAACAGCACGCACCTTGGCTAGG
TTTCTGAGCAACACCTCATTTCAGGGCCGCACAGGGGCTGTGTGGGTGGCAGGCTCCTCT
CAGGTGCATGTGTCTCGGCATTTCAAGGTATGGAGCTTACGCAGGGACCCGCTGGGTGCC
CCAGCCTGGGCAACAGTAGGCAGCTGGCAGGATGGACAGCTGGACTTCCAGCCAGGGGCG
GCTGCTCTCCGAGTTCCATCTCCATCTGGCACCCAGGCCCGGCCAAAGCTGCGAGTGGTA
ACTCTGGTGGAACACCCATTTGTGTTCACCAGGGAATCTGATGAAGATGGGCAGTGCCCG
GCTGGGCAGCTGTGTCTAGACCCAGGCACCAATGACTCGGCCAGGCTGGATGCGCTCTTC
ACTGCATTGGAGAATGGCTCCGTGCCTCGCACCCTGAGAAGATGCTGTTATGGCTACTGC
ATTGACCTGCTGGAGCGGCTGGCCGAGGACCTGGCCTTTGACTTTGAGCTCTATATTGTG
GGGGATGGCAAGTACGGGGCCCTGCGTGATGGACGCTGGACAGGCCTGGTGGGTGACCTG
CTGGCCGGTCGGGCACACATGGCCGTGACCAGCTTCAGTATCAACTCGGCTCGCTCCCAG
GTGGTGGATTTCACCAGCCCTTTCTTCTCCACCAGCCTGGGCATCATGGTGCGCACGCGA
GATACAGCCTCACCCATTGGGGCTTTCATGTGGCCCCTGCACTGGTCCATGTGGGTGGGT
GTGTTTGCTGCCCTGCACCTCACAGCGCTCTTCCTTACTCTGTACGAATGGCGGAGTCCC
TACGGGCTCACGCCACGCGGCCGCAACCGTGGTACCGTCTTCTCCTACTCCTCCGCTCTC
AACCTCTGCTACGCCATTCTCTTCGGACGCACTGTCTCCAGTAAGACACCCAAGTGTCCT
ACCGGACGCTTCCTCATGAATCTCTGGGCAATCTTCTGCCTGCTGGTGCTATCCAGTTAC
ACAGCCAACCTGGCAGCTGTCATGGTCGGGGACAAGACATTTGAGGAGCTGTCTGGAATC
CATGATCCCAAGCTGCACCACCCTTCCCAAGGCTTCCGCTTTGGCACCGTGTGGGAGAGC
AGCGCGGAGGCCTACATCAAGGCGAGCTTCCCCGAGATGCACGCACACATGCGTCGCCAC
AGCGCACCTACCACTCCACACGGAGTGGCCATGCTCACGAGCGACCCGCCCAAGCTCAAC
GCCTTCATCATGGATAAATCACTACTGGATTATGAGGTGTCCATAGATGCGGACTGCAAG
CTGCTCACCGTGGGCAAACCCTTTGCGATCGAGGGCTACGGCATAGGGCTGCCCCAAAAT
TCGCCGCTCACCTCCAACCTGTCAGAGTTCATCAGTAGGTACAAGTCCTCAGGCTTCATT
GATCTGCTCCATGACAAGTGGTACAAGATGGTGCCTTGCGGGAAGCGGGTGTTCGCCGTG
ACGGAGACGCTGCAGATGGGGGTCTACCACTTGTCAGGGTTGTTTGTCCTGCTGTGCCTC
GGGCTGGGCAGTGCACTTCTCACCTCGCTGGGTGAGCACGTCTTCTACCGCCTGGTGCTG
CCGCGCATCCGCAGGGGCAATAAGCTGCAGTATTGGCTTCACACGAGCCAGAAGATCCAC
CGAGCCCTCAACACAGGGCCACCAGAGGGGCAACAGGAGAGGGCAGAGCAGGAGTGCAGC
GGCCCCAAGGAGGAGCAACCTGCAGCCGACGGTGCGGGGCGCTGGAGGCGGGTGCGCCGG
GCCGTGGTGGAACGGGAACGGCGCGTGCGTTTCCTGCTGGAACCTGGGGAGGCTGGCGGG
GACCATCCGTGGCTCTGCTCCAATGGGCCCGGGGTGCAAGCAGAACTGCGGGAGCTGGAG
CTGCGCATTGAGGCTGCACGGGAGCGGCTGCGTAGTGCGCTGCTGCGGCGAGGGGAACTG
CGGGCCCAGCTTGGGGATGGCACCCGGCTCAGGCCACTGCGCCTGCTGCATGCGGCGCCC
GCCGAGAGC
Protein sequence predicted based on nucleotide sequence
MECVQTLWLSLALALARGSWVVRGHPQPCGVPTRAGASVRLAALLPRAPAARARVLAALA TPSPRLPHNLSLELVAVASPTRDPASLARGLCQVLAPPGVVASITFPEARPELRLLQFLA AATETPVLSVLRREVRAPLGAPTPFHLQLDWASPLETILDVLVSLVRAHAWEDIALVLCR VRDPSGLVTLWTSRASQAPKFVLDLSQLDSGNDSLRATLALLGTLEGGGTPVSAAVLLGC STAHAHEVLEAAPPGPQWLLGTPLPAEALPKTGLPPGVLVLGETGQPSLEAAVHDMVELV ARALSSMALMHPERALLPAAVNCEDLKTGGSESTARTLARFLSNTSFQGRTGAVWVAGSS QVHVSRHFKVWSLRRDPLGAPAWATVGSWQDGQLDFQPGAAALRVPSPSGTQARPKLRVV TLVEHPFVFTRESDEDGQCPAGQLCLDPGTNDSARLDALFTALENGSVPRTLRRCCYGYC IDLLERLAEDLAFDFELYIVGDGKYGALRDGRWTGLVGDLLAGRAHMAVTSFSINSARSQ VVDFTSPFFSTSLGIMVRTRDTASPIGAFMWPLHWSMWVGVFAALHLTALFLTLYEWRSP YGLTPRGRNRGTVFSYSSALNLCYAILFGRTVSSKTPKCPTGRFLMNLWAIFCLLVLSSY TANLAAVMVGDKTFEELSGIHDPKLHHPSQGFRFGTVWESSAEAYIKASFPEMHAHMRRH SAPTTPHGVAMLTSDPPKLNAFIMDKSLLDYEVSIDADCKLLTVGKPFAIEGYGIGLPQN SPLTSNLSEFISRYKSSGFIDLLHDKWYKMVPCGKRVFAVTETLQMGVYHLSGLFVLLCL GLGSALLTSLGEHVFYRLVLPRIRRGNKLQYWLHTSQKIHRALNTGPPEGQQERAEQECS GPKEEQPAADGAGRWRRVRRAVVERERRVRFLLEPGEAGGDHPWLCSNGPGVQAELRELE LRIEAARERLRSALLRRGELRAQLGDGTRLRPLRLLHAAPAES
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
177
End on reference sequence
3188
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
