DNASU Plasmid | Moxd2 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Moxd2

Gene Name: monooxygenase, DBH-like 2

Original Clone ID:

FLH270609.01L

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00083533

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1857nts

Open reading frame: 1 to 1857

ATGGCCTGTGTTCTACTCTTCAGACTTTTCTTACTTTTGGTCCTGGCAGCCTTTTCTCAA
GGCAAGCGCCTGGGTCCTACATCTCCCCTTCGTTATTCCAGGTTCCTAGATCCTTCCCGT
GCTGTTTTCCTGCGCTGGGACTTTGACTATGAGGCTGAGATAATCACATTTGAGCTCCAA
GTCCAAACAACTGGCTGGGTTGGCCTGGGCATCACAGACCGGTACACCTTTGTGGGAAGT
GATCTGGTAGTTGGAGGAGTCCTACCCAATGGCAATGTCTACTTTTCGGATCAGCACCTT
CTGGATGAAGACACTCTAGAACAGGATGGGAGCCAGGATGCTGAACTCCTAAGGCTCACA
GAAGATGCTGTCTCGACCACCATGCGCTTTTCCAGGCCTTTCAGAACCTGTGATCCACAT
GATCGTGACATTACGAGTGATACCATGAGGGTCCTGGCTGCCTATGGCCCGGATGACATA
CCAAAGATGAGTCGGGAGCATACTTTTGTCAAGTCCATCTTCTTGCTTCAAATGCTACAA
TATGATGATCAAGATGCCCCTGAAGACACCATCATCCATGACTTGAAGATCAGTAATTTC
ATCATTCCAGAGGATGATACCACATATGCCTGTACCTTTCTCCCACTCCCTATTGTCAGC
AAAAAGCATCACATCTACAAGTTTGAACCTATATTGGTGGAACGCAATGAGACGATGGTG
CATCACGTTCTCGTGTATGCATGTGGCAATTCTAGTGTGCTCCCCACAGGCATCGGCGAA
TGCTATGGATCAGACCCTGCCTTCTCTCTCTGCTCCCACGTCATCGCGGGCTGGGCTGTC
GGGGGCCTTAGTTACCAGTTTCCAGATGATGTGGGCATCTCTATTGGAACCCCCTTTGAC
CCTCAGTGGATCAGACTGGAAATTCACTACAGCAATTTTCAGAACCTTCCTGGTATCCGT
GATACCTCAGGGATGCGGCTGTTCTACACCTCGCACCTTCGCAAATATGACATGGGAGTC
CTCCAGCTGGGCATCTCAGTTTTCCCTATTCACTTTATACCCCCGGGTGCAGAGGCTTTC
TTGTCCTATGGACTATGCAAAACAGACAAGTTTGAAGAGCTGAATGGGGCTCCAGTATCT
GACATATATATATCAGCCTGCCTGCTCCACACCCACTTAGCTGGGCGGTCACTGCAAGCT
CTGCAATACAGAAATGGAACCCAACTCCAAGTAGTATGTAAAGATTTTTCCTATGATTTC
AATCTGCAAGAGTCTCGGGACTTACCTCATCCTGTGGTGATTAAGCCGGGGGATGAACTG
CTGATAGAATGTCATTACCAGACACTGGATCGTGACTTCATGACTTTTGGAGGTGCCAGC
ACCATTAATGAGATGTGCCTCATCTTCTTCTTCTACTATCCCAGAATTAACATCTCCAGT
TGCATGGGATACCCTGATATCATCTATGTGACCAATGAGCTGGGGGAAGAAGCATCAGAG
AATCCCATGGAGAACCTGATGGTCCTGGATAATGTGGAGTGGACTCCAGAGAACATTAAG
AAAGCTGAGAAAGCCTGCAAGGAGTCCCAGCAGACAGTATTGATCAAGACCATTGATGAA
GAAGTAGAAAATACAACAGGTTGGATTCCTGATATTATACCAACTCCTCGAGGACCATGC
TTAGAGTCTACTGGAGGCAAGGTGGAACCTCAGGACAATACCCCGGCAGGCTTCAGGGCT
GTACCACTGGCCCTCTCTGGCTCCAATACTGCTACTCTGAGGCCCCTGCCCATGATTGCT
GTACTGTTCCTGCAGGGTAGCCTCTCGTGTCTCCTTGCAATGCTGCAGACTGGAGTA

Protein sequence predicted based on nucleotide sequence

MACVLLFRLFLLLVLAAFSQGKRLGPTSPLRYSRFLDPSRAVFLRWDFDYEAEIITFELQ
VQTTGWVGLGITDRYTFVGSDLVVGGVLPNGNVYFSDQHLLDEDTLEQDGSQDAELLRLT
EDAVSTTMRFSRPFRTCDPHDRDITSDTMRVLAAYGPDDIPKMSREHTFVKSIFLLQMLQ
YDDQDAPEDTIIHDLKISNFIIPEDDTTYACTFLPLPIVSKKHHIYKFEPILVERNETMV
HHVLVYACGNSSVLPTGIGECYGSDPAFSLCSHVIAGWAVGGLSYQFPDDVGISIGTPFD
PQWIRLEIHYSNFQNLPGIRDTSGMRLFYTSHLRKYDMGVLQLGISVFPIHFIPPGAEAF
LSYGLCKTDKFEELNGAPVSDIYISACLLHTHLAGRSLQALQYRNGTQLQVVCKDFSYDF
NLQESRDLPHPVVIKPGDELLIECHYQTLDRDFMTFGGASTINEMCLIFFFYYPRINISS
CMGYPDIIYVTNELGEEASENPMENLMVLDNVEWTPENIKKAEKACKESQQTVLIKTIDE
EVENTTGWIPDIIPTPRGPCLESTGGKVEPQDNTPAGFRAVPLALSGSNTATLRPLPMIA
VLFLQGSLSCLLAMLQTGV

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 1860

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Moxd2

Symbol Nomenclature :

Moxd2

SYNONYM :

Dbhl1

Designation :

DBH-like monooxygenase protein 2|dopamine beta-hydroxylase-like 1|dopamine-beta-hydroxylase|dopamine-beta-hydroxylase-like protein

Full Nomenclature :

monooxygenase, DBH-like 2

GENEID :

194357

GI :

157169801

GenBank Accession :

BC152917

Mouse Genome Informatics :

2388042

Vega :

OTTMUSG00000026863

Target GenBank:

NM_139296

Protein

SMART domain :

DoH : Possible catecholamine-binding domain present in a variety of eukaryotic proteins.

UniProt :

Q7TT41

Clone

Cloning Information :

270609

HIP Master Clone ID :

202111

IMAGE ID :

100016168

Original Clone ID :

FLH270609.01L

Organism

Species Specific ID:

194357

Genome

Chromosome :

Map Location :

6 B1|6

Ensembl :

ENSMUSG00000029885

Reagents

Labome :

Moxd2-siRNA