Nphs1 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  Nphs1
Gene Name:  nephrosis 1, nephrin
Original Clone ID:
FLH270617.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083541
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3768nts
Open reading frame: 1 to 3768

ATGGGAGCTAAGGAAGCCACAGTCAGAGGCCCCGGGGCAAGCCCAGTGCACAGAACTTGC
CACCTGATTCCCCTGCTGCTCGCGGGAATGCTGACCACAGGCCTGGCCCAGTCGCCAGTC
CCCACCTCAGCACCTCGAGGCTTCTGGGCTCTATCTGAAAACCTGACTGTGGTGGAAGGG
TCGACAGTTAAGCTGTGGTGTGGCGTCAGGGCCCCCGGCAGTGTGGTGCAGTGGGCTAAG
GATGGGCTGCTTCTGGGTCCAAACCCTAAGATTCCAGGCTTCCCAAGGTACAGCCTGGAA
GGAGACAGTGCTAAAGGTGAGTTCCACCTGCTTATTGAAGCCTGTGACCTCAGCGATGAT
GCGGAGTACGAGTGCCAAGTCGGCCGCTCCGAGTTGGGTCCCGAGCTCGTGTCTCCCAGC
GTAATCCTCTCCATCCTAGTTTCCCCCAAGGTGCTTCAGTTAACCCCTGAGGCAGGAAGC
ACAGTTACCTGGGTAGCTGGACAGGAATATGTGGTCACCTGTGTGTCTGGGGATGCAAAG
CCAGCACCTGACATCATCTTCATCCAGGGTGGACGCACGGTAGAGGATGTCTCCTCCAGC
GTGAATGAGGGATCAGAGGAGAAACTCTTCTTCACGGAAGCCGAAGCCAGGGTGACACCC
CAGAGCTCAGATAATGGGCAGCTGCTAGTCTGCGAGGGGTCCAACCCAGCCTTGGCCACT
CCCATAAAGGCTTCGTTCACCATGAATATCCTGTTCCCCCCAGGACCTCCTGTCATTGAT
TGGCCAGGCCTGAATGAGGGGCATGTACGGGCAGGGGAGAACCTGGAGCTACCCTGCATA
GCCAGAGGTGGAAATCCACCTGCGACCCTGCAGTGGCTGAAGAATGGTAAACCAGTGTCC
ATAGCTTGGGGCACAGAGCATGCCCAGGCAGTGGCTCACAGTGTGCTGGTGATGACCGTT
CGACCTGAAGACCACGGAGCTCGGCTCAGCTGTCAGTCCTACAACAGTGTGTCTGCAGAG
ACCCAGGAGCGAAGCATCACTCTGCAGGTCACCTTTCCCCCCAGTGCCGTTACCATCCTG
GGATCTACATCACAGTCTGAGAACAAAAATGTGACCCTTTGCTGCCTTACCAAGTCCAGT
CGCCCACGGGTCCTGCTGCGATGGTGGTTGGGTGGACGGCAGTTGCTGCCCACGGATGAG
ACAGTCATGGATGGCCTGCATGGTGGCCACATCTCCATGTCCAATCTGACACTCTTGGTG
AAGAGAGAAGACAATGGCCTGTCCCTCACCTGCGAAGCCTTCAGTGATGCCTTCAGCAAG
GAGACCTTCAAGAAGTCACTCACCTTGAATGTAAAATACCCGGCCCAGAAGCTGTGGATT
GAGGGACCCCCAGAGGGGCAGAGCATCCGGACTGGAACTCGGGTGAGGCTGGTATGCTTG
GCCATTGGAGGCAACCCAGAGCCCTCCCTCACCTGGCTTAAGGATTCGCGCCCGGTGAAC
GATCCTCGGCAGTCTCAGGAGCCCCGGCGTGTGCAGCTGGGCAGTGTGGAGAAGTCCGGC
AGCACTTTCTCCCGCGAGCTGGTGCTGATCATAGGCCCGCCGGACAACCTAGCCAAGTTC
TCCTGCAAGGCGGGTCAGCTCAGTGCGTCTACGCAGCTGGTGGTGCAGTTCCCCCCAACA
AACCTGACCATCCTGGCCAACTCGTCCGCACTGCGCCCAGGCGACGCCTTGAACTTGACC
TGCGTCAGCATCAGCAGCAACCCTCCAGTTAACTTGTCTTTGGACAAGGAAGGAGAGAGG
CTGGACGACGTGGCTGCAAAACCCCAGAGCGCCCCATTCAAAGGCTCCGCTGCATCCAGG
AGTGTTTTTCTTCGGGTGTCATCCCGAGACCACGGTCACCGGGTTACCTGCCGGGCCCAC
AGCGAGGCACTTCGTGAAACCGTGAGCTCTTTCTACCGCCTCAACGTGTTATACCCTCCA
GAGTTCCTGGGAGAGCAAGTGCGGGCAGTGACCGTGGTGGAGCAGGGCCAGGCACTGCTG
CCTGTGTCCGTGTCTGCTAACCCCGCCCCCGAGGCCTTCAACTGGACCTTCCGAGGCTAC
CGCCTCAGCCCAGCTGGGGGTCCCCGGCACCGCATCCTGTCTGGAGGGGCACTGCAGCTG
TGGAATGTAACCCGAGCTGACGATGGCTTTTATCAGCTACACTGCCAGAACTCGGAGGGC
ACCGCCGAGGCGCTGTTGAAGCTGGACGTGCATTATGCTCCCACCATCCGTGCCCTGAAG
GACCCTACTGAGGTGAATGTTGGGGGTTCTGTGGATATAGTCTGCACCGTCGATGCCAAT
CCCATCCTCCCAGAGATGTTCAGCTGGGAGAGACTGGGGGAAGACGAGGAGGAACTGAAC
CTGGACGACATGGAGAAGATGTCAAAGGGATCCACAGGGCGTCTGCGGATTCGCCAGGCC
AAGCTGTCCCAGGCTGGCGCTTACCAGTGCATCGTGGACAACGGGGTGGCTCCCGCAGCC
AGAGGACTGGTTCGTCTTGTTGTCCGATTTGCCCCCCAGGTGGATCATCCTACTCCCCTG
ACGAAAGTGGCTGCTGCTGGGGACAGCACCAGCTCAGCCACACTCCACTGCCGTGCCCGG
GGGGTCCCCAACATCGACTTCACTTGGACCAAAAATGGGGTCCCTCTGGATCTCCAAGAC
CCCAGGTACACAGAGCACAAGTACCACCAGGGTGTGGTCCACAGCAGCCTCCTGACCATT
GCTAATGTGTCTGCAGCCCAGGACTATGCCCTCTTCAAATGCACAGCCACCAATGCCCTT
GGTTCAGACCACACCAACATCCAGCTCGTCAGCATCAGCCGTCCTGACCCTCCACTGGGG
CTGAAGGTTGTGAGCGTGAGCCCTCACTCGGTAGGACTGGAGTGGAAACCTGGCTTTGAT
GGGGGTCTGCCTCAGAGGTTCCAAATCAGGTATGAGGCCCTCGAGAGCCCAGGATTCCTC
TACATGGATGTCCTGCCTGCACAGGCCACCACCTTCACACTGACTGGGCTGAAGCCTTCT
ACACGGTACAGGATCTGGCTGTTGGCCAGCAATGCCCTGGGGGACAGTGGATTGACGGAC
AAGGGGATCCAGGTCTCCATCACTACCCCAGGCCTGGACCAGGCTCCTGAAGACACAGAC
CAGCCGCTGCCCACAGAGCAGCCTCCGGGACCCCCGAGGCTGCCCCTGCTGCCTGTGCTC
TTTGCGGTTGGTGGTCTTCTGTTGCTTTCCAATGCCTCCTGTGTTGGGGGACTCCTCTGG
CGGAGAAGACTGAGGCGCCTTGCTGAGGAGATCTCAGAGAAGACAGAGGCAGGGTCGGAG
GAGGATCGAATCAGGAATGAATATGAGGAGAGTCAGTGGACTGGGGACCGGGACACAAGA
AGCTCCACGGTTAGCACAGCAGAAGTGGACCCACACTACTACTCCATGAGGGACTTCAGT
CCCCAGCTTCCCCCAACACTGGAAGAGGTGTCATATCGCCAAGCCTTCACAGGTATTGAA
GATGAGGACATGGCCTTCCCCGGACACCTGTATGACGAGGTGGAGAGAGTCTATGGCCCA
CCTGGGGTCTGGGGACCCCTCTATGATGAAGTACAAATGGACCCCTATGACCTTCGCTGG
CCTGAGGTCAAATATGAGGACCCAAGGGGAATCTACGACCAGGTGGCGGCAGACATGGAT
GCTGGGGAGCCTGGTTCTCTGCCCTTTGAGCTGAGGGGACATCTGGTG

Protein sequence predicted based on nucleotide sequence

MGAKEATVRGPGASPVHRTCHLIPLLLAGMLTTGLAQSPVPTSAPRGFWALSENLTVVEG
STVKLWCGVRAPGSVVQWAKDGLLLGPNPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDD
AEYECQVGRSELGPELVSPSVILSILVSPKVLQLTPEAGSTVTWVAGQEYVVTCVSGDAK
PAPDIIFIQGGRTVEDVSSSVNEGSEEKLFFTEAEARVTPQSSDNGQLLVCEGSNPALAT
PIKASFTMNILFPPGPPVIDWPGLNEGHVRAGENLELPCIARGGNPPATLQWLKNGKPVS
IAWGTEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAETQERSITLQVTFPPSAVTIL
GSTSQSENKNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETVMDGLHGGHISMSNLTLLV
KREDNGLSLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQSIRTGTRVRLVCL
AIGGNPEPSLTWLKDSRPVNDPRQSQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNLAKF
SCKAGQLSASTQLVVQFPPTNLTILANSSALRPGDALNLTCVSISSNPPVNLSLDKEGER
LDDVAAKPQSAPFKGSAASRSVFLRVSSRDHGHRVTCRAHSEALRETVSSFYRLNVLYPP
EFLGEQVRAVTVVEQGQALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQL
WNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALKDPTEVNVGGSVDIVCTVDAN
PILPEMFSWERLGEDEEELNLDDMEKMSKGSTGRLRIRQAKLSQAGAYQCIVDNGVAPAA
RGLVRLVVRFAPQVDHPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTWTKNGVPLDLQD
PRYTEHKYHQGVVHSSLLTIANVSAAQDYALFKCTATNALGSDHTNIQLVSISRPDPPLG
LKVVSVSPHSVGLEWKPGFDGGLPQRFQIRYEALESPGFLYMDVLPAQATTFTLTGLKPS
TRYRIWLLASNALGDSGLTDKGIQVSITTPGLDQAPEDTDQPLPTEQPPGPPRLPLLPVL
FAVGGLLLLSNASCVGGLLWRRRLRRLAEEISEKTEAGSEEDRIRNEYEESQWTGDRDTR
SSTVSTAEVDPHYYSMRDFSPQLPPTLEEVSYRQAFTGIEDEDMAFPGHLYDEVERVYGP
PGVWGPLYDEVQMDPYDLRWPEVKYEDPRGIYDQVAADMDAGEPGSLPFELRGHLV

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3857
Start on reference sequence 87
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable