DNASU Plasmid | 6230417E10Rik (M. musculus )<br>In pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Shc4

Gene Name: SHC (Src homology 2 domain containing) family, member 4

Original Clone ID:

FLH270626.01L

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00083550

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1878nts

Open reading frame: 1 to 1878

ATGCGAGAACGCAGCCAGGACAGCCAGGCAGGACTCACGCTGTATGTAGGACTCTTTGGG
CACCTGGGGATGCTTCACAGGACCAAGTACAGCCGCTTTCGGAACGAGTCCATCACGTCT
TTGGACGAAGGGAGCCCCGGAGGCTCCGTTGGAAACAAGGGCTCGTCGCCGCCTCCCTAC
CCAGCCCTGGCCCCTCACCTGCCGACTGAGGATGCCACTGTGTCCAGCCAGGAGAGCCCC
ACGGCGCTGTGCACCTTGATTCCCCGTATGGCAAGCATGAAGCTGGCCAACCCCATCACT
TTTCTGGGTCTGAAAACCTTCTGCTTGGGTACGAAACAGGTGAGCCGGTTGAAGCTCCAA
GAGAACCAGGATCAGACTCCAAGCCGTCCAGCGTCCCCAGAATCCAATCTAAACAGGACT
GGACCTGCACCTGCACCGGATCCGGACCAGGTGGGACGCAGGCCCACCTCTTTGCGTCCT
GATACTTGCCCACTGCCTGGCCCTGGAGAACCAAGCCCCAGGAGCAAGCAGGACGGGCCC
CCTCTGCAGCACCTGTTGGGGAACGGTCTGAACTACTGTGTGCGGTACATGGGCTGCATT
GAAGTGCTGCAATCAATGAGGTCACTGGATTTTGGAATGAGAACCCAAGTTACAAGGGAA
GCAATAAGTCGCCTGTGTGAAGCTGTCCCCGGGGCCCATGGAGCCATTAAAAAACGAAAG
GCTCCCGTTAAGTTCCTGACAACGGTTCTTGGCAAAAGCAATCTTCAGTTTTCAGGAATG
AATATAAAACTGACTGTTTCAACAAGCAGCCTCACACTGATGAACCTTGACAACCAACAG
ATTATTGCAAATCACCAAATGCAATCGATTTCATTTGCCTCTGGAGGGGACCCTGATACC
ACAGACTATGTTGCCTATGTAGCTAAAGATCCAGTCAACCAACGAGCGTGCCACATACTG
GAGTGTCGCAGTGGAATGGCCCAAGACGTCATAAGCACCATAGGGCAGGCCTTTGAACTC
CGGTTCAAACAGTACTTGAAAAATCCTTCCTTGAACACTTGGGAAAGGGAGGAGGTACTT
GTTGATGGTGCCCCTGAAGACAGAGACCATGACTATTACAACTCCATCCCGGGGAAGCAG
CCACCTGAAGGCGGCATTTCAGATGTGCGCATCCAGGCTCAAGCTACAGACCAAATGGCA
TACTGCCCTATCCGGTGTGAAAAGTTGTGCTATTTGCCTGGGAACTCCACGTGCAGCGGT
GTGTATAAGAACTGTATGGGACGGAGCAGGCCAATAGGTATCCCGCACGAGAGAGCGGGG
CAGGGTGACACACCATCGCTGAGGCACTTCTGGAGAGTGGATCTCTTTGACGACCCCTGC
TATGTCAATACACAGGCTCTTCAGAGTATGCACAGCTATGCTGGAAATCAAAGCTCAGCT
CTGCCACAGGGGAGCCCCTGGCACCTCGGAAAGGCACCAGAAACTGTGCAGCCTGGAGCC
ACGGCCAAGCCTGGGAGCGCACTCGCTTTGCCACACATTAGGCAGCAGCTGTGGGACGAG
GAGTGCTTCCATGGCAAGCTGAGCAGAGGGGCAGCGGAGAAACTTTTGGTGAAGGATGGG
GACTTTTTGGTTCGAGAGAGTGTGACATCTCCCGGCCAGTTTGTACTGAGTGGACTGCAA
GGTGGCCAGGCCAAGCATCTTCTCTTGGTGGATCCAGAAGGCAAGGTGAGGACCAAGGAC
CATGTGTTTGATAATGTTGGCCACCTTATCAAATACCATATGGATAATAATTTGCCAATC
ATCTCTTCTGGAAGTGAAGTACGCCTTAAACAACCAATAAGAAAATATGATAACACAGGA
CTTTTGCCCCCCAAGAAA

Protein sequence predicted based on nucleotide sequence

MRERSQDSQAGLTLYVGLFGHLGMLHRTKYSRFRNESITSLDEGSPGGSVGNKGSSPPPY
PALAPHLPTEDATVSSQESPTALCTLIPRMASMKLANPITFLGLKTFCLGTKQVSRLKLQ
ENQDQTPSRPASPESNLNRTGPAPAPDPDQVGRRPTSLRPDTCPLPGPGEPSPRSKQDGP
PLQHLLGNGLNYCVRYMGCIEVLQSMRSLDFGMRTQVTREAISRLCEAVPGAHGAIKKRK
APVKFLTTVLGKSNLQFSGMNIKLTVSTSSLTLMNLDNQQIIANHQMQSISFASGGDPDT
TDYVAYVAKDPVNQRACHILECRSGMAQDVISTIGQAFELRFKQYLKNPSLNTWEREEVL
VDGAPEDRDHDYYNSIPGKQPPEGGISDVRIQAQATDQMAYCPIRCEKLCYLPGNSTCSG
VYKNCMGRSRPIGIPHERAGQGDTPSLRHFWRVDLFDDPCYVNTQALQSMHSYAGNQSSA
LPQGSPWHLGKAPETVQPGATAKPGSALALPHIRQQLWDEECFHGKLSRGAAEKLLVKDG
DFLVRESVTSPGQFVLSGLQGGQAKHLLLVDPEGKVRTKDHVFDNVGHLIKYHMDNNLPI
ISSGSEVRLKQPIRKYDNTGLLPPKK

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 2652

Start on reference sequence 772

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Shc4

Symbol Nomenclature :

Shc4

SYNONYM :

6230417E10Rik; 9930029B02Rik; Gm685; RaLP

Designation :

Full Nomenclature :

SHC (Src homology 2 domain containing) family, member 4

GENEID :

271849

Locus Tag :

RP23-323D9.1

GI :

157170391

GenBank Accession :

BC152904

Mouse Genome Informatics :

2655364

Vega :

OTTMUSG00000015349

Target GenBank:

NM_199022

Protein

SMART domain :

SH2 : Src homology 2 domains

SMART domain :

PTB : Phosphotyrosine-binding domain, phosphotyrosine-interaction (PI) domain

UniProt :

Q6S5L9

UniProt :

Q8CB26

Clone

Cloning Information :

270626

HIP Master Clone ID :

202128

IMAGE ID :

100016130

Original Clone ID :

FLH270626.01L

Organism

Species Specific ID:

271849

Genome

Chromosome :

Map Location :

2 F1|2

Ensembl :

ENSMUSG00000035109

Reagents

Labome :

Shc4-antibody

6230417E10Rik (M. musculus )In pENTR223.1 (Gateway donor/master vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Protein

Clone

Organism

Genome

Reagents

6230417E10Rik (M. musculus )
In pENTR223.1 (Gateway donor/master vector)