DNASU Plasmid | F830045P16Rik (M. musculus )<br>In pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: F830045P16Rik

Gene Name: RIKEN cDNA F830045P16 gene

Original Clone ID:

FLH270628.01L

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00083552

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1461nts

Open reading frame: 1 to 1461

ATGCAGAAGACCTTGGCACCAGCTGCTCTGCCTTATGTCACAGACAGGGAAACTGAGGCC
CAGAAAAGGAGATGGATTTTTCTACTCTTGTTTGTTGGGAAGCCTTTCATCACCCTGATA
GGCCCAGATCAGAGGACAACCCCTTCCAGTTCAGTGTTTTTTCTCTGTACTGCTGCCCCT
TTCAGGTCCATGAACCTCAGTGTCAACTGGTTGAGGAACAACAATGAACATCCAGCCTCA
ACCACACACGTGGTACATGTCAACAATGACTCTTACTCTCTCAGAAGCAAAGCATGGGTG
ACCCTGACCAAGCAAGATATCTCGTCTCAGATCACCTGTGAAGTCACCCATGGAGACCTC
AAAGAGCCCCTAAGAATGACCATTAATCTTTCTCAAGTTCTCCGAGTTATCCCAACACTG
AAGATCACCACTGAAGACCCAGAGATCCAAGATCATGTGCATCAAAAAGTGAACCTGACT
TGCCATGTAAACCACTTCTATCCCCAAAACATGGACCTCATCTGGACTAAGAATGGGCAC
AAGATCCCGACCATGGAACTTCCTCAGACCACCAGAAACTCTGATGGGACTTACTCTCTA
CAGCACACATTACAGGTAGATGCCATATTGAATAAGACTGACTTTGTCTGCTGGGTGATC
CAGGATGACCAGCCTCCAGTTAAGAACAGCATCACCCTGGGAGCTCCCAGGAAGATCAGA
GGAAGGACAGAGTATTCTCATCACCTTGAAGGCCCCCTGCAGCGGTTTCCACCTGGAGCA
AGCATCCAATTGAAGTACACATCCTCTGCATTACAAACTCGTCAGGTTACTGTGACCTGG
CTCAAAAACAACCACACCCGTCTCCAGACCCAGACCAACGTCTTCTCCAATGGAGAGACC
TACAATGTTACTAGCACTGCGATCGTCTCACTGGAGAGTGATGACATATTATCGTTAGTT
CTCTGCCTGGTTGAGCACAAGTCATTAGTGGTTTTCCAGAAAGTCATCTACTTGGGCCAG
TACCTCTGTGTTCCCCCGGCAGTGAGAGTGTCCCAATCTTCAACTGTCTCCAGTTTGGTG
ACTGTCACTTGCCATGTGCAGAGATTCTATCCCCAGGAGGTATATCTCTCCTGGCTGGAA
GACTGCCATGTGATCAGGAGAATAGAGCAACCTACACCTAAGAGGAATGAGGATGGGTCC
TACACTCTGGAAGTCCTGCAGTTGGTAAACACATCAGTGGAGAGGTCTGATCAAGTACTC
ACTTGCAAGGTGGAACATGAGGAACACCCTCCCATCCAGGCCAGCATTGTATTGTCTGCA
GCATCCTTTCCCACATCCAAAGCCATCAGGAGCCTAAGTTCAGAGAAATCTATCCACGTT
TTTGTGGCCTCCCTTCTGTGCCTCAAGTTACTTCTGGTGGTGAGTTTACTAGTCATCTAC
GTGTATAGGCGGTGGAGCCTG

Protein sequence predicted based on nucleotide sequence

MQKTLAPAALPYVTDRETEAQKRRWIFLLLFVGKPFITLIGPDQRTTPSSSVFFLCTAAP
FRSMNLSVNWLRNNNEHPASTTHVVHVNNDSYSLRSKAWVTLTKQDISSQITCEVTHGDL
KEPLRMTINLSQVLRVIPTLKITTEDPEIQDHVHQKVNLTCHVNHFYPQNMDLIWTKNGH
KIPTMELPQTTRNSDGTYSLQHTLQVDAILNKTDFVCWVIQDDQPPVKNSITLGAPRKIR
GRTEYSHHLEGPLQRFPPGASIQLKYTSSALQTRQVTVTWLKNNHTRLQTQTNVFSNGET
YNVTSTAIVSLESDDILSLVLCLVEHKSLVVFQKVIYLGQYLCVPPAVRVSQSSTVSSLV
TVTCHVQRFYPQEVYLSWLEDCHVIRRIEQPTPKRNEDGSYTLEVLQLVNTSVERSDQVL
TCKVEHEEHPPIQASIVLSAASFPTSKAIRSLSSEKSIHVFVASLLCLKLLLVVSLLVIY
VYRRWSL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 141

End on reference sequence 1604

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

F830045P16Rik

Symbol Nomenclature :

F830045P16Rik

SYNONYM :

Sirpb3

Designation :

novel immunoglobulin C1-set domain containing protein|uncharacterized protein LOC228592

Full Nomenclature :

RIKEN cDNA F830045P16 gene

GENEID :

228592

Locus Tag :

RP23-384K11.5

GI :

157169785

GenBank Accession :

BC152751

Mouse Genome Informatics :

3045317

Vega :

OTTMUSG00000015525

Target GenBank:

NM_177653

Protein

SMART domain :

IGc1 : Immunoglobulin C-Type

UniProt :

Q8BJ95

Clone

Cloning Information :

270628

HIP Master Clone ID :

202130

IMAGE ID :

100015953

Original Clone ID :

FLH270628.01L

Organism

Species Specific ID:

228592

Genome

Chromosome :

Map Location :

2 F1|2

Ensembl :

ENSMUSG00000043727

Reagents

Labome :

F830045P16Rik-siRNA

F830045P16Rik (M. musculus )In pENTR223.1 (Gateway donor/master vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Protein

Clone

Organism

Genome

Reagents

F830045P16Rik (M. musculus )
In pENTR223.1 (Gateway donor/master vector)