TM0002 (T. maritima ) in pMH1 (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  TM0002
Gene Name:  hypothetical protein
Original Clone ID:
6986
Keyword:
None
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH1
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
Next amino acid codon after CDS represents STOP, hence clone should be labeled for CDS 'fusion', overall 'closed'
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
TmCD00083889
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 126nts
Open reading frame: 37 to 162

ATGGGATCTGATAAAATTCATCATCATCATCATCACATGTCCCCCGAAGACTGGAAAAGG
CTTATATGTTTCCATACCTCTAAGGAAGTATTGAAACAAACTCTCGACGATGCACAGCAG
AACATCTCCTACAGTGTTTCCATACCTCTAAGGAAGTATTAAGTTTAAACGGTCTCCAG

Protein sequence predicted based on nucleotide sequence

MSPEDWKRLICFHTSKEVLKQTLDDAQQNISYSVSIPLRKY*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 126
Mutations:
None
Discrepancy:
g94t,D32Y;
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DL41
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable