Gene:
Gene Symbol:
TM0024
Gene Name:
laminarinase
Original Clone ID:
6999
Keyword:
None
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH2T7
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
Next amino acid codon after CDS represents STOP, hence clone should be labeled for CDS 'fusion', overall 'closed'
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
Joint Center for Structural Genomics
TmCD00083897
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1968nts
Open reading frame: 37 to 2004
ATGGGATCTGATAAAATTCATCATCATCATCATCACATGATGAGCAGGCTGGTTTTCGCT
CTGCTTCTCTTTCCTGTTTTCATTCTGGCTCAAAACATCCTTGGCAACGCTTCTTTCGAT
GAACCAATTCTCATCGCAGGTGTGGATATAGACCCACCCGCAGAGGATGGCTCTATAGAC
ACAGGAGGAAACTGGGTATTCTTCACCAATTCAAACGGTGAGGGAACGGCTCGAGTCGAA
AACGGCGTTCTCGTGGTTGAGATAACAAACGGAGGAGATCACACCTGGTCGGTTCAGATC
ATACAGGCTCCCATACGTGTTGAGAAACTCCACAAGTACAGAGTTTCTTTCCGAGCCAAG
GCTTCCTCTCAAAAGAACATCGGGGTGAAGATAGGAGGAACGGCCGGAAGAGGATGGACC
GCGTACAACCCCGGTACCGACGAATCCGGCGGCATGGTCTTCGAGCTCGGAACAGATTGG
CAGAAGTACGAGTTCGAATTCGTCATGAGACAGGAGACCGATGAAAATGCTCGTTTCGAG
TTTCAGCTTGGAAGGTATACCGGCACGGTCTGGATAGACGACGTAGTGATGGAGGACATC
GGTGTTCTCGAGGTAAGCGGTGAGGAAAACGAAATCTACACCGAGGAGGATGAAGACAAA
GTGGAAGACTGGCAGCTCGTTTGGAGTCAGGAGTTCGATGACGGTGTTATCGATCCGAAC
ATCTGGAACTTCGAGATAGGAAACGGTCATGCAAAAGGTATTCCAGGCTGGGGTAACGGG
GAACTCGAGTACTATACAGACGAAAACGCGTTCGTTGAGAACGGCTGTCTTGTGATTGAG
GCAAGAAAAGAACAGGTTTCCGATGAGTACGGAACCTACGACTACACCTCAGCCAGGATG
ACCACAGAAGGAAAATTCGAAATAAAGTACGGAAAAATCGAAATAAGGGCAAAACTTCCA
AAAGGAAAAGGTATCTGGCCCGCTCTCTGGATGCTCGGAAACAACATAGGAGAGGTCGGA
TGGCCCACCTGTGGTGAGATAGACATCATGGAAATGCTTGGCCACGACACCAGAACCGTT
TATGGAACAGCACACGGTCCGGGATATTCTGGTGGTGCGAGTATAGGTGTTGCCTATCAT
CTTCCAGAAGGAGTTCCTGATTTCTCCGAAGACTTCCACATTTTCTCCATCGAGTGGGAC
GAAGACGAAGTGGAGTGGTACGTGGACGGACAGCTCTACCACGTCCTCAGCAAGGATGAA
CTGGCCGAACTCGGTCTTGAGTGGGTTTTCGACCATCCGTTCTTCCTCATTCTGAACGTT
GCCGTGGGAGGCTACTGGCCGGGTTATCCCGACGAAACCACCCAATTCCCGCAGAGAATG
TACATCGACTACATCAGAGTCTATAAAGATATGAATCCGGAAACAATCACCGGGGAAGTG
GATGACTGCGAATATGAACAAGCACAGCAGCAGGCAGGTCCCGAGGTGACCTATGAACAG
ATAAATAACGGCACTTTCGACGAACCTATTGTGAACGATCAGGCCAACAACCCGGACGAA
TGGTTCATTTGGCAGGCGGGAGATTACGGGATCAGCGGTGCCAGGGTCTCCGATTACGGT
GTCAGGGATGGCTACGCTTATATCACGATAGCCGATCCTGGAACTGACACGTGGCATATT
CAGTTCAACCAGTGGATAGGTCTTTACAGAGGAAAAACCTACACCATTTCTTTCAAAGCA
AAAGCGGATACACCAAGACCTATAAATGTGAAAATTCTGCAGAATCACGATCCCTGGACC
AACTATTTTGCTCAAACGGTGAATCTCACAGCGGACTGGCAGACGTTCACGTTCACCTAC
ACGCATCCAGACGATGCGGATGAGGTCGTTCAGATCAGTTTCGAACTCGGAGAAGGAACG
GCAACTACGATTTATTTCGATGATGTCACGGTGAGCCCTCAATAATTAATTAACTTTAAG
AAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAG
Protein sequence predicted based on nucleotide sequence
MMSRLVFALLLFPVFILAQNILGNASFDEPILIAGVDIDPPAEDGSIDTGGNWVFFTNSN GEGTARVENGVLVVEITNGGDHTWSVQIIQAPIRVEKLHKYRVSFRAKASSQKNIGVKIG GTAGRGWTAYNPGTDESGGMVFELGTDWQKYEFEFVMRQETDENARFEFQLGRYTGTVWI DDVVMEDIGVLEVSGEENEIYTEEDEDKVEDWQLVWSQEFDDGVIDPNIWNFEIGNGHAK GIPGWGNGELEYYTDENAFVENGCLVIEARKEQVSDEYGTYDYTSARMTTEGKFEIKYGK IEIRAKLPKGKGIWPALWMLGNNIGEVGWPTCGEIDIMEMLGHDTRTVYGTAHGPGYSGG ASIGVAYHLPEGVPDFSEDFHIFSIEWDEDEVEWYVDGQLYHVLSKDELAELGLEWVFDH PFFLILNVAVGGYWPGYPDETTQFPQRMYIDYIRVYKDMNPETITGEVDDCEYEQAQQQA GPEVTYEQINNGTFDEPIVNDQANNPDEWFIWQAGDYGISGARVSDYGVRDGYAYITIAD PGTDTWHIQFNQWIGLYRGKTYTISFKAKADTPRPINVKILQNHDPWTNYFAQTVNLTAD WQTFTFTYTHPDDADEVVQISFELGEGTATTIYFDDVTVSPQ*LINFKKEIYIPG*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
1929
Mutations:
None
Discrepancy:
ins@1926,39;
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DL41
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pMH2T7
Synonyms:
pMH-2-T7
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.
Comments:
None
Size (bp):
4084
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColEI origin of replication
1921
2603
promoter
T7
T7 promoter
290
310
promoter
araC
araC promoter
256
285
selectable marker
AmpR
ampicillin resistance gene
1164
1823
tag
His
N-terminal 6x His tag
367
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
