DNASU Plasmid | TM0056 (T. maritima ) in pMH2T7 (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: TM0056

Gene Name: peptide ABC transporter substrate-binding protein

Original Clone ID:

7023

Keyword:

None

Species:

Thermotoga maritima

Type:

cDNA

Vector Name:

pMH2T7

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

Next amino acid codon after CDS represents STOP, hence clone should be labeled for CDS 'fusion', overall 'closed'

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Joint Center for Structural Genomics

TmCD00083899

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1983nts

Open reading frame: 37 to 2019

ATGGGATCTGATAAAATTCATCATCATCATCATCACGTGTTAGTTGTGAGAAGGTTGTTT
GTTTTGCTGTCGCTTGTGTTCATGGTTGTTTTAGCTCTTGCTGCCAACGACACATGGGTC
TTCTACGCAACACCGGAAGAGTACTACAAGGCTACAGGAAAGAAGATTACCGAGTACCAT
GAATCGCCGATGCTGACCAAGCTCGTCGAAGAAGGAAAGCTTCCACCCGTCGAACAGAGA
CTCCCGGAGGAACCGCTCGTGGTTCAGCCTGTTGAAAAAGTTGGACAGTTCGGTGGTACC
TGGAGAAGAGTCTGGAAAGGGCCTTCTGACAGGTGGGGTATTTCCAAGCTCATCGAAGTG
AAACTCGCGTTCTGGGACAAAGAGGGTGGAAAACTCGTTCCGGGGCTCGCGAAGAGCTGG
GAGGTTCTGGAGAACGGAAGGGTCTACATCTTCCATCTGAGAAAGGGTGTGAAGTGGTCC
GATGGAGCACCGTACACGGCCCACGATATCGTGTTCTGGGTTAACGACATCGTAGGAAAC
GACGATATCACGCCTTCGAAACCTGACTGGTACAACATTGGTGTGAAAGTCGAAGCGCTC
GACGATTACACGGTGAAGTTCGAATTCAGCAAGCCTTATGGATTGTTCCTTCTGAAAGTT
CCATACGGTGGATTTACCGGAGCACCAGCACACTATCTGAAACAGTTCCATCCAAAGTAC
ACACCGATGGAAGAAATAGAGAAGAAGATGGTGGAAGGTGTGCACAACACCTGGGTGGAC
CTCTTCAACGATAAAAACGACTTCCTTGAAAACACCGAGCTTCCAACACTATCACCGTGG
AAGCCTATCACCGATCCAACAGAACAGTTCTACATACTCGAGAGAAACCCGTACTTCTGG
GCGGTTGATATCGAAGGTAATCAGCTTCCATACATCGATTACGTGAGGCACGAATACGTC
AAGAACGACGAAGTCATACTCCTGAAAGCGATCTCCGGTGAAATCGATATGCAGTGGAGA
CATATCGGAGGACTGGGAGCGGGAGCAGGAAACTTCACACTGCTCATGGAGAACAGCCAG
AGTGGAGGATACAGAGTGCTAAAATGGATCGCTGCGAACGGTTCTGCCAGCAGAATCTCA
CTAAACTACGCTCACTCCGACGAGGTGCTGAGGAAGGTCTTCAACGATGTAAGGTTCAGG
CAGGCTCTCTCACTCGCTATCAACAGGGAAGAAATCAACGAGATTCTCTTCAACGGTCTC
GCTGAGCCAAGGCAGGCGTCTCTCGTGAGTGGATCCCCATACTTCGATCCCGAATGGGAA
AAAGCTTATGCAGAGTACGATCCAGACAGAGCGAACAAGCTTCTCGATGAAATGGGATTG
AAGTGGGATGACAAGCACGAATACAGACTCTTACCAGATGGCAGACCACTCCGATTCACC
ATCACTGTGACTGGGCAGTTTCATGTTGACGTCTGGACGATGGTAAAAGAATACTGGAAA
CAGATAGGTGTCTGGGTGGAGATTGAGAACCTTGAAAGATCTCTCTTCTACGAAAGAGCC
GATGCCGGTGACTTCGACGCGATGGTGTGGAACATGGATAGGGCTGCCCAACCACTCTCT
TCACCGATGGTCATCTTCCCGGGTTCCGAGAACATAGCAGACTTCTGGTACATCGGATGG
AGTGGCTGGATCTCATACTACATCGACAAGAACATAAGAGGTGTGGAGCCCGAAGAAGTA
CCCGAAGGGCCTGAACCACCAGAGGTCGTCTACAGACTTGTCGATCTGTACTACCAGATA
GCCTCCACGCCGGATCCTGATAAGATCAAAGAGCTCATGGCAGAAGCGACGAAGATCCAT
AGAGAAAATCTCTGGATGATAGGAACCGTCGGAGAAGACCTTTCGCCTGCCATAGCGAAG
AACAACTTCAGAAACGTACCGGAATTCCTCGTAACGGACGATGTGTTGAGAACTCCTCTG
AATGCCATGCCGATGCAGTTCTTCATCGAACAGAAATAAGTTTAAACGGTCTCCAG

Protein sequence predicted based on nucleotide sequence

VLVVRRLFVLLSLVFMVVLALAANDTWVFYATPEEYYKATGKKITEYHESPMLTKLVEEG
KLPPVEQRLPEEPLVVQPVEKVGQFGGTWRRVWKGPSDRWGISKLIEVKLAFWDKEGGKL
VPGLAKSWEVLENGRVYIFHLRKGVKWSDGAPYTAHDIVFWVNDIVGNDDITPSKPDWYN
IGVKVEALDDYTVKFEFSKPYGLFLLKVPYGGFTGAPAHYLKQFHPKYTPMEEIEKKMVE
GVHNTWVDLFNDKNDFLENTELPTLSPWKPITDPTEQFYILERNPYFWAVDIEGNQLPYI
DYVRHEYVKNDEVILLKAISGEIDMQWRHIGGLGAGAGNFTLLMENSQSGGYRVLKWIAA
NGSASRISLNYAHSDEVLRKVFNDVRFRQALSLAINREEINEILFNGLAEPRQASLVSGS
PYFDPEWEKAYAEYDPDRANKLLDEMGLKWDDKHEYRLLPDGRPLRFTITVTGQFHVDVW
TMVKEYWKQIGVWVEIENLERSLFYERADAGDFDAMVWNMDRAAQPLSSPMVIFPGSENI
ADFWYIGWSGWISYYIDKNIRGVEPEEVPEGPEPPEVVYRLVDLYYQIASTPDPDKIKEL
MAEATKIHRENLWMIGTVGEDLSPAIAKNNFRNVPEFLVTDDVLRTPLNAMPMQFFIEQK
*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 1983

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DL41

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pMH2T7

Synonyms:

pMH-2-T7

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.

Comments:

None

Size (bp):

4084

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGTGTAATTAATTAACTTTAAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColEI origin of replication

1921

2603

promoter

T7 promoter

290

310

promoter

araC

araC promoter

256

285

selectable marker

AmpR

ampicillin resistance gene

1164

1823

tag

His

N-terminal 6x His tag

367

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

Gene

Gene Symbol :

TM0056

GENEID :

896880

Locus Tag :

TM0056

Target GenBank:

NP_227872

Protein

Structural Annotation Wiki :

TOPSAN

Clone

Cloning Information :

271524

HIP Master Clone ID :

202234

Original Clone ID :

7023

Experimental

TargetTrack - Experimental data :

281937

Organism

Species Specific ID:

896880