TM0064 (T. maritima ) in pMH2T7 (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  TM0064
Gene Name:  glucuronate isomerase
Original Clone ID:
7026
Keyword:
None
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH2T7
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
Next amino acid codon after CDS represents STOP, hence clone should be labeled for CDS 'fusion', overall 'closed'
Mutation / Discrepancy:
No / Yes
Publications:
PMID: 12945057
Title: Crystal structure of uronate isomerase (TM0064) from Thermotoga maritima at 2.85 A resolution.
PMID: 19762644
Title: Three-dimensional structural view of the central metabolic network of Thermotoga maritima.
Authors:
PSI
Joint Center for Structural Genomics
TmCD00083921
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1356nts
Open reading frame: 55 to 1410

ATGGGATCTGATAAAATTCATCATCATCATCATCACGTGGTCGACTCGAGCTTAATGTTT
CTTGGAGAGGATTACCTTCTGACAAACAGAGCAGCTGTGCGATTGTTCAATGAGGTCAAG
GATCTTCCCATTGTGGATCCACACAACCATCTGGATGCGAAAGACATCGTTGAGAACAAA
CCCTGGAACGATATCTGGGAAGTAGAGGGTGCGACAGATCATTATGTGTGGGAACTCATG
AGACGCTGTGGTGTTTCGGAAGAGTACATAACTGGATCGAGAAGTAACAAAGAAAAATGG
CTTGCCCTCGCAAAGGTGTTTCCAAGATTTGTGGGAAATCCAACCTACGAGTGGATACAC
CTTGATCTCTGGAGAAGGTTCAACATAAAGAAGGTAATTTCAGAAGAGACGGCAGAAGAG
ATATGGGAAGAAACGAAAAAGAAGCTTCCTGAGATGACACCTCAGAAACTTCTCAGAGAC
ATGAAGGTGGAGATCCTTTGCACAACAGATGATCCTGTTTCCACTCTGGAACATCACAGG
AAGGCAAAAGAAGCGGTGGAAGGTGTTACCATCCTTCCCACCTGGAGACCAGACAGAGCA
ATGAACGTAGACAAAGAGGGATGGAGAGAGTACGTGGAGAAGATGGGGGAGCGTTACGGT
GAAGACACCTCTACACTCGATGGTTTTCTGAACGCGCTCTGGAAGTCACACGAACATTTC
AAAGAGCATGGTTGTGTGGCGAGTGACCATGCTCTCCTGGAGCCATCGGTATACTATGTG
GACGAAAACAGAGCAAGGGCAGTTCATGAAAAAGCCTTCAGTGGGGAGAAACTCACTCAG
GATGAAATAAACGATTACAAGGCCTTTATGATGGTTCAGTTTGGGAAAATGAACCAGGAA
ACGAACTGGGTGACACAGCTTCACATCGGTGCTTTGAGAGATTATAGAGACAGCCTTTTC
AAAACGCTCGGACCGGACTCAGGAGGAGATATTTCGACGAACTTTCTGAGAATCGCAGAA
GGGCTGAGGTACTTTCTGAACGAGTTCGACGGAAAGCTCAAGATCGTTCTCTATGTACTG
GATCCCACGCATCTTCCAACGATATCCACCATTGCACGTGCTTTTCCGAATGTTTATGTC
GGTGCTCCATGGTGGTTCAACGACAGTCCATTTGGCAGGGAGATGCACCTGAAATACCTC
GCTTCAGTCGATCTTCTGTACAACCTTGCGGGAATGGTCACAGATTCCAGAAAACTTCTC
TCCTTCGGTTCCAGAACAGAGATGTTCAGGAGGGTTCTTTCGAATGTGGTGGGAGAGATG
GTTGAAAAGGGGCAGATTCCTATTAAGGAAGCAAGAGAACTCGTGAAGCACGTTAGTTAC
GATGGCCCGAAAGCTCTCTTCTTTGGATAATTAATTAACTTTAAGAAGGAGATATACATA
CCCGGGTAAGTTTAAACGGTCTCCAG

Protein sequence predicted based on nucleotide sequence

MFLGEDYLLTNRAAVRLFNEVKDLPIVDPHNHLDAKDIVENKPWNDIWEVEGATDHYVWE
LMRRCGVSEEYITGSRSNKEKWLALAKVFPRFVGNPTYEWIHLDLWRRFNIKKVISEETA
EEIWEETKKKLPEMTPQKLLRDMKVEILCTTDDPVSTLEHHRKAKEAVEGVTILPTWRPD
RAMNVDKEGWREYVEKMGERYGEDTSTLDGFLNALWKSHEHFKEHGCVASDHALLEPSVY
YVDENRARAVHEKAFSGEKLTQDEINDYKAFMMVQFGKMNQETNWVTQLHIGALRDYRDS
LFKTLGPDSGGDISTNFLRIAEGLRYFLNEFDGKLKIVLYVLDPTHLPTISTIARAFPNV
YVGAPWWFNDSPFGREMHLKYLASVDLLYNLAGMVTDSRKLLSFGSRTEMFRRVLSNVVG
EMVEKGQIPIKEARELVKHVSYDGPKALFFG*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1356
Mutations:
None
Discrepancy:
c1026g,L342L;t1124g,M375R;
ins@1353,39;
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DL41
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable