Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00840326
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 6267nts
Open reading frame: 1 to 6267
ATGGAGGACACCCAGGCCATTGACTGGGACGTTGAGGAGGAGGAGGAGACAGAGCAATCC
AGTGAATCGTTGAGATGTAACGTGGAGCCCGTAGGGCGGCTACATATCTTTAGTGGTGCT
CATGGACCAGAAAAAGATTTCCCACTACACCTCGGGAAGAATGTGGTCGGCCGAATGCCT
GACTGCTCTGTGGCCCTGCCATTTCCATCTATCTCTAAACAACATGCCGAGATCGAAATC
TTAGCCTGGGATAAGGCACCTATTCTCCGAGACTGTGGGTCCCTTAATGGTACTCAAATC
CTGCGACCTCCCAAGGTTCTGAGTCCCGGGGTGAGTCACCGTCTCAGGGACCAGGAGTTA
ATTCTGTTTGCCGACTTGCTCTGCCAGTACCATCGCCTGGACGTTTCCCTGCCTTTTGTC
TCCCGCGGCCCTCTGACAGTCGAAGAGACACCTAGAGTACAGGGCGAAACTCAACCACAG
AGGCTTTTGTTGGCTGAGGACTCGGAGGAGGAAGTAGATTTTCTTTCTGAAAGGCGTATG
GTAAAAAAATCCAGGACCACATCTTCATCCGTGATAGTGCCAGAGAGCGATGAAGAGGGG
CATTCCCCGGTCCTCGGAGGCCTCGGACCGCCTTTTGCCTTCAATTTGAACAGCGATACA
GATGTCGAAGAAGGTCAGCAACCAGCTACAGAGGAGGCATCCTCCGCCGCCAGAAGAGGT
GCGACTGTAGAGGCAAAGCAGTCTGAGGCTGAGGTTGTAACTGAAATCCAGCTTGAAAAG
GATCAGCCTTTAGTGAAGGAGAGGGATAATGATACAAAAGTCAAGCGGGGTGCAGGGAAT
GGGGTGGTTCCAGCTGGGGTGATTTTAGAGAGGTCCCAACCTCCTGGAGAGGATAGCGAC
ACCGATGTCGATGATGACTCAAGGCCACCTGGAAGGCCCGCCGAGGTCCATTTGGAGCGG
GCTCAGCCGTTTGGCTTCATCGACTCCGACACTGATGCGGAAGAAGAGAGGATCCCCGCA
ACCCCAGTTGTCATTCCTATGAAGAAGCGCAAGATCTTCCACGGAGTGGGTACACGCGGA
CCTGGAGCACCAGGACTGGCCCATTTGCAGGAGTCCCAGGCCGGTAGTGATACCGATGTG
GAAGAAGGCAAGGCCCCACAGGCCGTCCCTCTGGAGAAATCCCAAGCTTCCATGGTTATT
AACAGCGACACAGATGACGAAGAAGAAGTCTCAGCAGCCCTGACTTTGGCACACCTGAAA
GAGAGTCAGCCTGCTATCTGGAACAGGGATGCCGAAGAGGACATGCCCCAACGTGTCGTC
CTTCTGCAGCGCAGCCAAACAACCACTGAGAGAGACAGTGACACCGACGTCGAGGAGGAA
GAGCTCCCCGTGGAAAATAGAGAAGCTGTGCTCAAGGATCACACAAAGATTAGGGCCCTT
GTTAGAGCACATTCAGAGAAGGACCAACCCCCTTTTGGGGACAGTGATGACAGTGTGGAA
GCCGATAAGAGCTCACCTGGGATCCACTTGGAAAGAAGCCAAGCCTCCACCACAGTGGAC
ATCAACACACAAGTTGAGAAGGAAGTCCCGCCAGGGTCAGCCATTATACATATAAAGAAA
CATCAGGTTTCTGTTGAGGGGACAAATCAAACTGATGTGAAAGCCGTTGGGGGACCAGCA
AAGCTGCTTGTGGTATCCCTAGAGGAAGCCTGGCCTCTGCATGGGGACTGTGAAACAGAT
GCCGAGGAGGGCACCTCTCTGACTGCATCAGTAGTTGCAGATGTAAGAAAGAGTCAGTTG
CCAGCCGAAGGGGATGCTGGGGCCGAGTGGGCTGCAGCTGTTCTTAAGCAGGAGAGAGCC
CATGAGGTTGGCGCCCAGGGTGGGCCACCCGTGGCACAAGTGGAGCAGGACCTCCCTATC
TCAAGAGAGAACCTGACAGATCTGGTGGTGGACACAGACACTCTAGGGGAATCCACCCAG
CCACAGCGAGAGGGAGCCCAGGTCCCGACAGGAAGGGAGAGAGAGCAACATGTGGGTGGG
ACCAAGGACTCCGAAGACAACTATGGTGATTCTGAGGATCTCGACCTACAAGCTACCCAG
TGCTTTTTGGAGAATCAGGGCCTGGAGGCAGTCCAGAGTATGGAGGATGAACCTACACAG
GCCTTCATGTTGACTCCACCTCAAGAGCTTGGCCCTTCCCATTGCAGCTTCCAGACAACA
GGTACACTAGATGAACCATGGGAGGTTCTCGCTACTCAGCCCTTCTGTCTTCGCGAGTCT
GAAGATTCTGAAACCCAGCCTTTTGACACGCACCTTGAGGCCTATGGACCTTGCTTGTCC
CCACCTAGGGCAATACCAGGAGATCAACATCCAGAGAGTCCAGTTCACACAGAGCCAATG
GGGATCCAGGGCAGAGGGAGGCAGACCGTGGATAAAGTGATGGGTATACCAAAAGAGACA
GCAGAGAGAGTGGGACCTGAGCGAGGGCCACTGGAAAGAGAAACCGAGAAACTGCTACCC
GAAAGGCAGACAGATGTGACAGGAGAAGAAGAATTGACCAAGGGGAAACAGGACCGAGAG
CAAAAGCAGTTGCTAGCTAGAGACACGCAGAGACAAGAATCTGACAAAAATGGGGAAAGT
GCAAGTCCTGAAAGGGATAGGGAAAGTTTGAAGGTAGAAATTGAAACCTCTGAGGAAATA
CAAGAAAAACAAGTACAGAAGCAGACCCTTCCAAGCAAAGCATTTGAGAGGGAAGTCGAG
CGACCAGTAGCCAACAGAGAGTGCGATCCAGCCGAGTTAGAAGAGAAGGTGCCTAAAGTC
ATCCTCGAGAGAGATACACAGAGGGGCGAGCCTGAGGGAGGGAGTCAGGACCAAAAAGGG
CAGGCCTCCAGCCCAACTCCAGAACCTGGGGTGGGCGCGGGGGACCTTCCGGGACCTACC
TCAGCTCCCGTGCCTTCTGGCAGCCAGTCAGGTGGCAGGGGATCTCCAGTGAGCCCCAGG
AGACATCAGAAAGGCCTCCTGAATTGCAAGATGCCACCCGCTGAAAAGGCTTCCAGGATC
AGAGCAGCTGAGAAGGTTTCCAGAGGCGATCAGGAATCCCCCGATGCTTGTCTGCCCCCT
ACAGTACCTGAAGCCCCAGCCCCACCGCAAAAGCCCCTTAACTCTCAGAGCCAGAAGCAT
CTGGCACCTCCCCCCCTTCTTTCTCCCTTGTTGCCTTCCATCAAGCCAACCGTTCGTAAG
ACAAGGCAAGATGGGTCCCAGGAAGCTCCCGAGGCTCCCCTGTCTTCAGAGTTGGAGCCT
TTCCACCCAAAGCCTAAAATTAGAACTCGGAAGTCCTCCAGAATGACACCCTTTCCAGCT
ACCTCTGCAGCACCTGAACCTCACCCTTCAACCTCCACAGCCCAGCCAGTTACTCCTAAG
CCGACCTCTCAGGCCACTAGGTCTAGGACAAATAGGTCCTCCGTCAAAACCCCTGAACCC
GTGGTCCCTACAGCCCCTGAATTACAGCCCTCAACCTCTACCGACCAGCCTGTGACATCT
GAACCCACGTCTCAAGTTACTAGGGGACGCAAAAGTAGATCTTCTGTTAAGACCCCGGAA
ACAGTTGTCCCAACAGCCCTTGAGCTCCAACCTAGCACATCCACGGACCGACCTGTGACC
TCTGAACCGACCTCGCAGGCTACCCGCGGAAGGAAAAATCGATCCTCCGTTAAGACTCCA
GAGCCTGTGGTGCCTACCGCACCCGAACTCCAGCCTTCAACATCTACCGATCAGCCCGTG
ACTTCGGAGCCTACCTATCAGGCTACTAGAGGCAGAAAAAATCGCTCCTCCGTGAAGACT
CCCGAACCAGTGGTGCCCACAGCACCAGAGCTCCGGCCTTCTACCTCCACCGACCGACCG
GTCACACCCAAGCCCACCTCTCGGACCACTAGGAGCAGAACAAATATGTCCTCGGTGAAG
ACGCCTGAAACTGTTGTTCCTACCGCCCCAGAACTGCAGATTTCCACCAGCACCGACCAA
CCTGTTACCCCTAAGCCCACTTCTCGCACCACAAGGAGTAGGACTAATATGTCTTCCGTC
AAGAACCCTGAATCAACCGTCCCTATCGCCCCTGAGTTACCACCCTCTACCTCTACTGAG
CAGCCTGTCACCCCCGAGCCCACATCTAGGGCTACTCGGGGACGAAAAAATAGATCCTCT
GGCAAGACACCTGAGACACTCGTCCCCACAGCGCCTAAGCTGGAGCCCTCCACTTCCACT
GACCAGCCGGTCACTCCTGAGCCAACATCACAGGCCACCAGAGGCCGGACCAATAGGTCT
TCAGTTAAGACACCGGAGACAGTCGTTCCCACAGCTCCTGAGTTGCAGCCATCCACCTCG
ACAGACCAGCCAGTGACGCCAGAGCCCACGTCGCAGGCCACAAGGGGCAGAACAGATAGA
TCCAGTGTGAAGACCCCAGAAACAGTGGTCCCCACTGCCCCAGAGCTACAGGCTTCTGCT
TCCACCGATCAACCTGTCACTTCTGAGCCGACATCTCGGACAACCAGGGGAAGAAAGAAT
CGGTCTTCTGTCAAGACTCCTGAAACCGTTGTGCCTGCCGCTCCTGAACTACAGCCTTCG
ACCTCGACTGACCAACCGGTTACCCCGGAGCCAACCAGTCGGGCAACTAGGGGCAGGACA
AACAGATCCTCGGTCAAGACGCCCGAATCAATTGTCCCTATAGCGCCTGAGCTTCAGCCT
TCCACCTCCAGGAACCAGCTCGTCACCCCTGAGCCCACCAGTCGCGCCACCAGGTGCAGA
ACCAACCGCTCCTCAGTGAAGACACCCGAGCCAGTTGTGCCCACCGCCCCCGAACCCCAT
CCTACCACCTCTACGGACCAGCCCGTCACACCTAAGCTCACATCTCGCGCTACTAGGAGA
AAAACCAACAGGTCCTCTGTCAAAACTCCCAAACCAGTTGAACCCGCAGCTTCTGATCTT
GAGCCTTTTACTCCCACAGACCAATCCGTCACTCCGGAGGCCATAGCTCAGGGTGGTCAG
AGTAAAACACTGAGGTCATCCACAGTAAGAGCTATGCCGGTTCCTACTACCCCCGAATTC
CAATCTCCCGTCACCACAGATCAGCCAATTTCACCTGAGCCTATTACTCAACCCAGTTGC
ATCAAGAGGCAGCGAGCCGCTGGGAACCCCGGCTCCCTGGCAGCTCCTATTGACCATAAG
CCTTGCTCCGCCCCCTTGGAACCTAAATCGCAGGCGTCAAGGAACCAAAGATGGGGAGCA
GTGAGAGCAGCCGAATCCCTTACAGCCATTCCTGAACCGGCTTCTCCCCAACTTCTTGAG
ACACCAATTCATGCCTCCCAGATCCAAAAGGTTGAGCCAGCAGGTAGATCTAGGTTCACA
CCGGAATTGCAGCCTAAGGCTTCTCAAAGCCGCAAGAGGTCTTTAGCTACCATGGATTCA
CCACCACATCAAAAACAGCCTCAAAGAGGCGAAGTCTCCCAGAAAACAGTGATTATCAAA
GAAGAGGAAGAAGATACTGCAGAGAAGCCAGGGAAGGAAGAGGATGTCGTTACTCCAAAA
CCAGGCAAGAGAAAGCGCGACCAGGCAGAGGAGGAGCCCAACAGAATACCAAGCCGCAGC
CTGCGCCGGACAAAACTTAACCAAGAATCAACAGCTCCCAAAGTGCTCTTCACAGGAGTG
GTGGATGCTCGGGGAGAACGGGCTGTGCTGGCACTCGGCGGAAGTCTCGCTGGTTCAGCG
GCAGAAGCTTCCCACCTGGTCACTGATCGCATCCGCCGGACCGTCAAGTTCCTGTGTGCC
CTCGGGCGGGGAATTCCTATTCTGTCCCTGGACTGGCTCCATCAGTCCCGCAAGGCTGGT
TTCTTCTTACCCCCGGATGAATATGTGGTCACCGACCCTGAACAGGAAAAGAACTTCGGC
TTTAGCCTTCAGGACGCCCTGAGCAGGGCCCGGGAGCGGAGGCTGCTGGAGGGATATGAG
ATCTATGTGACCCCTGGAGTCCAGCCCCCACCACCTCAGATGGGAGAGATTATTAGCTGC
TGTGGCGGCACATACCTACCCAGCATGCCTAGGTCCTATAAGCCCCAAAGAGTTGTGATC
ACTTGCCCTCAGGATTTCCCTCATTGCTCTATTCCACTGCGGGTCGGGCTGCCACTCCTC
TCGCCTGAGTTCCTGCTGACTGGAGTGCTGAAGCAAGAAGCTAAGCCCGAGGCATTTGTG
CTTTCCCCTCTAGAGATGTCATCTACC
Protein sequence predicted based on nucleotide sequence
MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMP DCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQEL ILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRM VKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRG ATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSD TDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRG PGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLK ESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRAL VRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKK HQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQL PAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQ PQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQ AFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLS PPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLP ERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEI QEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKG QASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRI RAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRK TRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPK PTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPE TVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPV TSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVK TPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTE QPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRS SVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASA STDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRT NRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPH PTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQ SKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHK PCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFT PELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPK PGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSA AEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFG FSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVI TCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
