Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00840436
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3657nts
Open reading frame: 1 to 3657
ATGCTGAGGAGAGTCACTGTTGCTGCAGTCTGTGCCACCCGGAGGAAGTTGTGTGAGGCC
GGGCGGGAGCTCGCGGCGCTCTGGGGAATCGAAACGCGGGGTCGGTGCGAAGACTCTGCT
GCTGCCAGACCCTTTCCTATTCTGGCCATGCCTGGAAGAAACAAGGCGAAGTCTACCTGC
AGCTGCCCTGACCTGCAGCCCAATGGACAGGATCTGGGCGAGAACAGCCGGGTTGCCCGA
CTAGGAGCGGATGAATCTGAGGAAGAGGGACGGAGGGGGTCCCTCAGTAATGCTGGGGAC
CCTGAGATCGTCAAGTCTCCCAGCGACCCCAAGCAATACCGATACATCAAATTACAGAAT
GGCTTGCAGGCACTTCTGATTTCAGACCTAAGTAATATGGAAGGTAAAACAGGAAATACA
ACAGATGATGAAGAAGAAGAGGAGGTGGAGGAAGAAGAAGAAGATGATGATGAAGATTCG
GGAGCTGAAATAGAAGATGACGATGAAGAGGGTTTTGATGATGAAGATGAGTTTGATGAT
GAACATGATGATGATCTTGATACTGAGGATAATGAACTGGAAGAATTAGAAGAGAGAGCA
GAAGCTAGAAAAAAAACTACTGAAAAACAGCAATTGCAGAGCCTGTTTTTGCTGTGGTCA
AAGCTGACTGATAGACTGTGGTTTAAGTCAACTTATTCAAAAATGTCTTCAACCCTGCTG
GTCGAGACAAGAAATCTTTATGGGGTAGTTGGAGCTGAAAGCCGGTCTGCACCTGTTCAG
CATTTGGCAGGATGGCAAGCGGAGGAGCAGCAGGGTGAAACTGACACAGTTCTGTCTGCA
GCGGCTCTTTGTGTTGGAGTTGGGAGTTTCGCTGATCCAGATGACCTGCCGGGGCTGGCA
CACTTTTTGGAGCACATGGTATTCATGGGTAGTTTAAAATATCCAGATGAGAATGGATTT
GATGCCTTCCTGAAGAAGCATGGGGGTAGTGATAATGCCTCAACTGATTGTGAACGCACT
GTCTTTCAGTTTGATGTCCAGAGGAAGTACTTCAAGGAAGCTCTTGATAGATGGGCGCAG
TTCTTCATCCACCCACTAATGATCAGAGATGCAATTGACCGTGAAGTTGAAGCTGTTGAT
AGTGAATATCAACTTGCAAGGCCTTCTGATGCAAACAGAAAGGAAATGTTGTTTGGAAGC
CTTGCTAGACCTGGACATCCTATGGGAAAATTTTTTTGGGGAAATGCTGAGACGCTCAAG
CATGAGCCAAGAAAGAATAATATTGATACACATGCTAGATTGAGAGAATTCTGGATGCGT
TACTACTCTTCTCATTACATGACTTTAGTGGTTCAATCCAAAGAAACACTGGATACTTTG
GAAAAGTGGGTGACTGAAATCTTCTCTCAGATACCAAACAATGGGTTACCCAGACCAAAC
TTTGGCCATTTAACGGATCCATTTGACACACCAGCATTTAACAAACTATATAGAGTTGTT
CCAATCAGAAAAATTCATGCTCTGACCATCACATGGGCACTTCCTCCTCAACAGCAACAT
TACAGGGTGAAGCCACTTCATTATATATCCTGGCTGGTTGGACATGAAGGCAAAGGCAGC
ATTCTTTCTTTCCTTAGGAAAAAATGCTGGGCTCTTGCACTGTTTGGTGGAAACGGTGAG
ACAGGATTTGAGCAAAATTCTACTTATTCAGTGTTCAGCATTTCTATTACACTGACTGAT
GAGGGTTATGAACATTTTTATGAGGTTGCTTACACTGTCTTTCAGTATTTAAAAATGCTG
CAGAAGCTAGGCCCAGAAAAAAGAATTTTTGAAGAGATTCGGAAAATTGAGGATAATGAA
TTTCATTACCAAGAACAGACAGATCCAGTTGAGTATGTGGAAAACATGTGTGAGAACATG
CAGCTGTACCCATTGCAGGACATTCTCACTGGAGATCAGCTTCTTTTTGAATACAAGCCA
GAAGTCATTGGTGAAGCCTTGAATCAGCTAGTTCCTCAAAAAGCAAATCTTGTTTTACTC
TCTGGTGCTAATGAGGGAAAATGTGACCTCAAGGAGAAATGGTTTGGAACTCAATATAGT
ATAGAAGATATTGAAAACTCTTGGGCTGAACTGTGGAATAGTAATTTCGAATTAAATCCA
GATCTTCATCTTCCAGCTGAAAACAAGTACATAGCCACGGACTTTACGTTGAAGGCTTTC
GATTGCCCGGAAACAGAATACCCAGTTAAAATTGTGAATACTCCACAAGGTTGCCTGTGG
TATAAGAAAGACAACAAATTCAAAATCCCCAAAGCATATATACGTTTCCATCTAATTTCA
CCGTTGATACAGAAATCTGCAGCAAATGTGGTCCTCTTTGATATCTTTGTCAATATCCTT
ACGCATAACCTTGCGGAACCAGCTTATGAAGCAGATGTGGCACAGCTGGAGTATAAACTG
GTAGCTGGAGAACATGGTTTAATTATTCGAGTGAAAGGATTTAACCACAAACTACCTCTA
CTGTTTCAGCTCATTATTGACTACTTAGCTGAGTTCAATTCCACACCAGCTGTCTTTACA
ATGATAACTGAGCAGTTGAAGAAGACCTACTTTAACATCCTCATCAAGCCCGAGACTTTG
GCCAAAGATGTACGGCTTTTAATCTTGGAATATGCCCGTTGGTCTATGATTGACAAGTAC
CAGGCTTTGATGGACGGCCTTTCCCTTGAGTCTCTGCTGAGCTTCGTCAAAGAATTCAAA
TCCCAGCTCTTTGTGGAGGGCCTGGTACAAGGGAATGTCACAAGCACAGAATCTATGGAT
TTCCTGAAATATGTTGTTGACAAACTAAACTTCAAGCCTCTGGAGCAGGAGATGCCTGTG
CAGTTCCAGGTGGTAGAGCTGCCCAGTGGCCACCATCTATGCAAAGTGAAAGCTCTGAAC
AAGGGTGATGCCAACTCTGAAGTCACTGTGTACTACCAGTCAGGTACCAGGAGTCTAAGA
GAATATACGCTTATGGAGCTGCTTGTGATGCACATGGAAGAACCTTGTTTTGACTTCCTT
CGAACCAAGCAGACCCTTGGGTACCATGTCTACCCTACCTGTAGGAACACATCCGGGATT
CTAGGATTTTCTGTCACTGTGGGGACTCAGGCAACCAAATACAATTCTGAAGTTGTTGAT
AAGAAGATAGAAGAGTTTCTTTCTAGCTTTGAGGAGAAGATTGAGAACCTCACTGAAGAG
GCATTCAACACCCAGGTCACAGCTCTCATCAAGCTGAAGGAGTGTGAGGATACCCACCTT
GGGGAGGAGGTGGATAGGAACTGGAATGAAGTGGTTACACAGCAGTACCTCTTTGACCGC
CTTGCCCACGAGATTGAAGCACTGAAGTCATTCTCAAAATCAGACCTGGTCAACTGGTTC
AAGGCCCATAGAGGGCCAGGAAGTAAAATGCTCAGCGTTCATGTTGTTGGATATGGGAAG
TATGAACTGGAAGAGGATGGTACCCCTTCTAGTGAGGATTCAAATTCTTCTTGTGAAGTG
ATGCAGCTGACCTACCTACCAACCTCTCCTCTGCTGGCAGATTGTATCATCCCCATTACT
GATATTAGGGCTTTCACAACAACACTCAACCTTCTCCCCTACCATAAAATAGTCAAA
Protein sequence predicted based on nucleotide sequence
MLRRVTVAAVCATRRKLCEAGRELAALWGIETRGRCEDSAAARPFPILAMPGRNKAKSTC SCPDLQPNGQDLGENSRVARLGADESEEEGRRGSLSNAGDPEIVKSPSDPKQYRYIKLQN GLQALLISDLSNMEGKTGNTTDDEEEEEVEEEEEDDDEDSGAEIEDDDEEGFDDEDEFDD EHDDDLDTEDNELEELEERAEARKKTTEKQQLQSLFLLWSKLTDRLWFKSTYSKMSSTLL VETRNLYGVVGAESRSAPVQHLAGWQAEEQQGETDTVLSAAALCVGVGSFADPDDLPGLA HFLEHMVFMGSLKYPDENGFDAFLKKHGGSDNASTDCERTVFQFDVQRKYFKEALDRWAQ FFIHPLMIRDAIDREVEAVDSEYQLARPSDANRKEMLFGSLARPGHPMGKFFWGNAETLK HEPRKNNIDTHARLREFWMRYYSSHYMTLVVQSKETLDTLEKWVTEIFSQIPNNGLPRPN FGHLTDPFDTPAFNKLYRVVPIRKIHALTITWALPPQQQHYRVKPLHYISWLVGHEGKGS ILSFLRKKCWALALFGGNGETGFEQNSTYSVFSISITLTDEGYEHFYEVAYTVFQYLKML QKLGPEKRIFEEIRKIEDNEFHYQEQTDPVEYVENMCENMQLYPLQDILTGDQLLFEYKP EVIGEALNQLVPQKANLVLLSGANEGKCDLKEKWFGTQYSIEDIENSWAELWNSNFELNP DLHLPAENKYIATDFTLKAFDCPETEYPVKIVNTPQGCLWYKKDNKFKIPKAYIRFHLIS PLIQKSAANVVLFDIFVNILTHNLAEPAYEADVAQLEYKLVAGEHGLIIRVKGFNHKLPL LFQLIIDYLAEFNSTPAVFTMITEQLKKTYFNILIKPETLAKDVRLLILEYARWSMIDKY QALMDGLSLESLLSFVKEFKSQLFVEGLVQGNVTSTESMDFLKYVVDKLNFKPLEQEMPV QFQVVELPSGHHLCKVKALNKGDANSEVTVYYQSGTRSLREYTLMELLVMHMEEPCFDFL RTKQTLGYHVYPTCRNTSGILGFSVTVGTQATKYNSEVVDKKIEEFLSSFEEKIENLTEE AFNTQVTALIKLKECEDTHLGEEVDRNWNEVVTQQYLFDRLAHEIEALKSFSKSDLVNWF KAHRGPGSKMLSVHVVGYGKYELEEDGTPSSEDSNSSCEVMQLTYLPTSPLLADCIIPIT DIRAFTTTLNLLPYHKIVK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
