Detailed Vector Information: pMCSG9

Explanation of Terms

Gene:
Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pMCSG9
Source:
M. Donnelly
Description:
Vector with tags for solubility of insert, with T7 promoter, adds N-terminal His tag, MBP, TEV, SNA; amp resistance; ligation independent cloning (LIC).
Comments:
None
Publications:
PMID: 16497515
Title: An expression vector tailored for large-scale, high-throughput purification of recombinant proteins
Authors:
M.Donnelly
Midwest Center for Structural Genomics
PSI
Midwest Center for Structural Genomics
EvNO00084273
Price: 
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AVAILABLE
No restriction
Special MTA: PSI

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.