Detailed Vector Information: pMCentr2

Explanation of Terms

Gene:
Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pMCentr2
Source:
M. Donnelly
Description:
cloning vector to link to Gateway system, with attL1-TEV-LIC site-attL2; zeocin resistance; ligation independent cloning to make pENTR vector for recombinational cloning
Comments:
None
Publications:
None
Authors:
M.Donnelly
Midwest Center for Structural Genomics
PSI
Midwest Center for Structural Genomics
EvNO00084276
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Special MTA: PSI

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.