cloning vector to link to Gateway system, with attL1-TEV-LIC site-attL2; zeocin resistance; ligation independent cloning to make pENTR vector for recombinational cloning
Comments:
None
Publications:
None
Authors:
M.Donnelly
Midwest Center for Structural Genomics
PSI
Midwest Center for Structural Genomics
EvNO00084276
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Special MTA:
PSI
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
cloning vector to link to Gateway system with attL1-TEV-LIC site-attL2; kanamycin resistance; ligation independent cloning to make pENTR vector for recombinational cloning