Detailed Vector Information: pVP33A

Explanation of Terms

Gene:
Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pVP33A
Source:
CESG
Description:
Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, tetra-cys, Tev cleavage; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte.
Comments:
None
Publications:
PMID: 16377204
Title: High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system
Authors:
University of Wisconsin
PSI
Center for Eukaryotic Structural Genomics
EvNO00084280
Price: 
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AVAILABLE
No restriction
Special MTA: PSI,Promega

Recommended Growth Condition:

Distributed in bacterial strain:
BR610
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.