Cell-free expression vector, adds N-terminal GST tag and 3CP cleavage site; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte.
Comments:
None
Publications:
PMID:15782178 Title: Cell-free protein production and labeling protocol for NMR-based structural proteomics PMID:21899277 Title: Amino acid determinants of substrate selectivity in the Trypanosoma brucei sphingolipid synthase family.
Authors:
University of Wisconsin
PSI
Center for Eukaryotic Structural Genomics