Gene:
Gene Symbol:
TEVgp1
Gene Name:
polyprotein
Original Clone ID:
None
Keyword:
None
Species:
Tobacco etch virus
Type:
cDNA
Vector Name:
pMHTDelta238
Format:
CLOSED
Source:
CESG
Description:
E. coli expression vector; expresses mutated form of MBP-T7 tagged TEV protease; MBP portion of the fusion is removed in vivo by autocleavage; kanamycin resistance
Comments:
expresses MBP-His tagged TEV protease
Mutation / Discrepancy:
Unknown / Unknown
Publications:
PMID: 17543538
Title: A combined approach to improving large-scale production of tobacco etch virus protease.
PMID: 23566202
Title: The intrinsically disordered membrane protein selenoprotein s is a reductase in vitro
PMID: 23159557
Title: Se enrichment of proteins expands the biological NMR toolbox
PMID: 22963794
Title: Expression and purification of the membrane enzyme selenoprotein K
PMID: 20064616
Title: Properties of a homogeneous C-lobe prepared by introduction of a TEV cleavage site between the lobes of human transferrin
PMID: 23566202
Title: The intrinsically disordered membrane protein selenoprotein s is a reductase in vitro
PMID: 24509858
Title: Stereochemical features of glutathione-dependent enzymes in the Sphingobium sp. strain SYK-6 beta-aryl etherase pathway
Title: A combined approach to improving large-scale production of tobacco etch virus protease.
PMID: 23566202
Title: The intrinsically disordered membrane protein selenoprotein s is a reductase in vitro
PMID: 23159557
Title: Se enrichment of proteins expands the biological NMR toolbox
PMID: 22963794
Title: Expression and purification of the membrane enzyme selenoprotein K
PMID: 20064616
Title: Properties of a homogeneous C-lobe prepared by introduction of a TEV cleavage site between the lobes of human transferrin
PMID: 23566202
Title: The intrinsically disordered membrane protein selenoprotein s is a reductase in vitro
PMID: 24509858
Title: Stereochemical features of glutathione-dependent enzymes in the Sphingobium sp. strain SYK-6 beta-aryl etherase pathway
Authors:
University of Wisconsin
PSI
Center for Eukaryotic Structural Genomics
PSI
Center for Eukaryotic Structural Genomics
TvCD00084286
Price:
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AVAILABLE
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Special MTA: PSI
Special MTA: PSI
Insert sequence: 714nts
Open reading frame: 81 to 713
GTGCCGGGCTGGAAGTTCTGTTCCAGGGGCCCGAAAACCTGTACTTCCAGGCGATCGCCC
ATCATCATCATCATCATCATGGAGAAAGCTTGTTTAAGGGGCCGCGTGATTACAACCCGA
TATCGAGCTCCATTTGTCATTTGACGAATGAATCTGATGGGCACACAACATCGTTGTATG
GTATTGGATTTGGTCCCTTCATCATTACAAACAAGCACTTGTTTAGAAGAAATAATGGAA
CACTGTTGGTCCAATCACTACATGGTGTATTCAAGGTCAAGGACACCACGACTTTGCAAC
AACACCTCGTTGATGGGAGGGACATGATAATTATTCGCATGCCTAAGGATTTCCCACCAT
TTCCTCAAAAGCTGAAATTTAGAGAGCCACAAAGGGAAGAGCGCATATGTCTTGTGACAA
CCAACTTCCAAACTAAGAGCATGTCTAGCATGGTGTCAGACACTAGTTGCACATTCCCTT
CATCTGATGGCATATTCTGGAAGCATTGGATTCAAACCAAGGATGGGCAGTGTGGCAGTC
CATTAGTATCAACTAGAGATGGGTTCATTGTTGGTATACACTCAGCATCGAATTTCACCA
ACACAAACAATTATTTCACAAGCGTGCCGAAAAACTTCATGGAATTGTTGACAAATCAGG
AGGCGCAGCAGTGGGTTAGTGGTTGGCGATTAAATGCTGACTCAGTATTGTGGG
Protein sequence predicted based on nucleotide sequence
GESLFKGPRDYNPISSSICHLTNESDGHTTSLYGIGFGPFIITNKHLFRRNNGTLLVQSL HGVFKVKDTTTLQQHLVDGRDMIIIRMPKDFPPFPQKLKFREPQREERICLVTTNFQTKS MSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFIVGIHSASNFTNTNNYFT SVPKNFMELLTNQEAQQWVSGWRLNADSVLW
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pMHTDelta238
Synonyms:
''
Sequencing Primer:
Forward:
pMHTDelta238-F
Reverse:
pMHTDelta238-R
Description:
E. coli expression vector; expresses a mutated and truncated form of TEV protease as an N-terminal fusion to MBP-His7; The MBP portion of the fusion is removed in vivo by autocleavage leaving His7-TEV; kanamycin resistance
Comments:
MBP portion of the TEV fusion is removed in vivo by autocleavage leaving a His tagged TEV
Size (bp):
5777
Parent Vector:
None
Properties:
bacterial expression, with tag/fusion/marker
Author Name:
Center for Eukaryotic Structural Genomics
University of Wisconsin
University of Wisconsin
Publications:
PMID:
17543538
Title: A combined approach to improving large-scale production of tobacco etch virus protease.
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR332 bacterial origin
3573
4802
promoter
NPTII
NPTII promoter for kanR gene
0
0
promoter
T5
T5 phage promoter
120
202
promoter
LacIq
promoter for LacI
2495
3563
protease cleavage site
TEV
TEV protease cleavage site
1383
1402
protease cleavage site
3C
3C protease cleavage site
1358
1382
repressor protein gene
LacI
Lac inhibitor
2403
2494
selectable marker
KanR
kanamycin resistance gene
4808
5603
tag
MBP
Maltose Binding Protein (MBP) tag
230
1338
tag
His
7xHis tag
1409
1430
trxn termination sequence
rrnB
T1 terminator rrnB
2218
2316
