DNASU Plasmid | MUT (Homo sapiens) in pVP16

Explanation of Terms

Gene:

Gene Symbol: MUT

Gene Name: methylmalonyl CoA mutase

Original Clone ID:

1265.1.43_GO.41458

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pVP16

Format:

CLOSED

Source:

Center for Eukaryotic Structural Genomics

Description:

None

Comments:

His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics

HsCD00084295

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 2274nts

Open reading frame: 28 to 2301

Reference Sequence Alignment

ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCTTAAGAGCTAAG
AATCAGCTTTTTTTACTTTCACCTCATTACCTGAGGCAGGTAAAAGAATCATCAGGCTCC
AGGCTCATACAGCAACGACTTCTACACCAGCAACAGCCCCTTCACCCAGAATGGGCTGCC
CTGGCTAAAAAGCAGCTGAAAGGCAAAAACCCAGAAGACCTAATATGGCACACCCCGGAA
GGGATCTCTATAAAACCCTTGTATTCCAAGAGAGATACTATGGACTTACCTGAAGAACTT
CCAGGAGTGAAGCCATTCACACGTGGACCATATCCTACCATGTATACCTTTAGGCCCTGG
ACCATCCGCCAGTATGCTGGTTTTAGTACTGTGGAAGAAAGCAATAAGTTCTATAAGGAC
AACATTAAGGCTGGTCAGCAGGGATTATCAGTTGCCTTTGATCTGGCGACACATCGTGGC
TATGATTCAGACAACCCTCGAGTTCGTGGTGATGTTGGAATGGCTGGAGTTGCTATTGAC
ACTGTGGAAGATACCAAAATTCTTTTTGATGGAATTCCTTTAGAAAAAATGTCAGTTTCC
ATGACTATGAATGGAGCAGTTATTCCAGTTCTTGCAAATTTTATAGTAACTGGAGAAGAA
CAAGGTGTACCTAAAGAGAAACTTACTGGTACCATCCAAAATGATATACTAAAGGAATTT
ATGGTTCGAAATACATACATTTTTCCTCCAGAACCATCCATGAAAATTATTGCTGACATA
TTTGAATATACAGCAAAGCACATGCCAAAATTTAATTCAATTTCAATTAGTGGATACCAT
ATGCAGGAAGCAGGGGCTGATGCCATTCTGGAGCTGGCCTATACTTTAGCAGATGGATTG
GAGTACTCTAGAACTGGACTCCAGGCTGGCCTGACAATTGATGAATTTGCACCAAGGTTG
TCTTTCTTCTGGGGAATTGGAATGAATTTCTATATGGAAATAGCAAAGATGAGAGCTGGT
AGAAGACTCTGGGCTCACTTAATAGAGAAAATGTTTCAGCCTAAAAACTCAAAATCTCTT
CTTCTAAGAGCACACTGTCAGACATCTGGATGGTCACTTACTGAGCAGGATCCCTACAAT
AATATTGTCCGTACTGCAATAGAAGCAATGGCAGCAGTATTTGGAGGGACTCAGTCTTTG
CACACAAATTCTTTTGATGAAGCTTTGGGTTTGCCAACTGTGAAAAGTGCTCGAATTGCC
AGGAACACACAAATCATCATTCAAGAAGAATCTGGGATTCCCAAAGTGGCTGATCCTTGG
GGAGGTTCTTACATGATGGAATGTCTCACAAATGATGTTTATGATGCTGCTTTAAAGCTC
ATTAATGAAATTGAAGAAATGGGTGGAATGGCCAAAGCTGTAGCTGAGGGAATACCTAAA
CTTCGAATTGAAGAATGTGCTGCCCGAAGACAAGCTAGAATAGATTCTGGTTCTGAAGTA
ATTGTTGGAGTAAATAAGTACCAGTTGGAAAAAGAAGACACTGTAGAAGTTCTGGCAATT
GATAATACTTCAGTGCGAAACAGGCAGATTGAAAAACTTAAGAAGATCAAATCCAGCAGG
GATCAAGCTTTGGCTGAACGTTGTCTTGCTGCACTAACCGAATGTGCTGCTAGCGGAGAT
GGAAATATCCTGGCTCTTGCAGTGGATGCATCTCGGGCAAGATGTACAGTGGGAGAAATC
ACAGATGCCCTGAAAAAGGTATTTGGTGAACATAAAGCGAATGATCGAATGGTGAGTGGA
GCATATCGCCAGGAATTTGGAGAAAGTAAAGAGATAACATCTGCTATCAAGAGGGTTCAT
AAATTCATGGAACGTGAAGGTCGCAGACCTCGTCTTCTTGTAGCAAAAATGGGACAAGAT
GGCCATGACAGAGGAGCAAAAGTTATTGCTACAGGATTTGCTGATCTTGGTTTTGATGTG
GACATAGGCCCTCTTTTCCAGACTCCTCGTGAAGTGGCCCAGCAGGCTGTGGATGCGGAT
GTGCATGCTGTGGGCGTAAGCACCCTCGCTGCTGGTCATAAAACCCTAGTTCCTGAACTC
ATCAAAGAACTTAACTCCCTTGGACGGCCAGATATTCTTGTCATGTGTGGAGGGGTGATA
CCACCTCAGGATTATGAATTTCTGTTTGAAGTTGGTGTTTCCAATGTATTTGGTCCTGGG
ACTCGAATTCCAAAGGCTGCCGTTCAGGTGCTTGATGATATTGAGAAGTGTTTGGAAAAG
AAGCAGCAATCTGTATAATAGGACCCAGCTTTCTTGTACAAAGTGGT

Protein sequence predicted based on nucleotide sequence

ENLYFQSLRAKNQLFLLSPHYLRQVKESSGSRLIQQRLLHQQQPLHPEWAALAKKQLKGK
NPEDLIWHTPEGISIKPLYSKRDTMDLPEELPGVKPFTRGPYPTMYTFRPWTIRQYAGFS
TVEESNKFYKDNIKAGQQGLSVAFDLATHRGYDSDNPRVRGDVGMAGVAIDTVEDTKILF
DGIPLEKMSVSMTMNGAVIPVLANFIVTGEEQGVPKEKLTGTIQNDILKEFMVRNTYIFP
PEPSMKIIADIFEYTAKHMPKFNSISISGYHMQEAGADAILELAYTLADGLEYSRTGLQA
GLTIDEFAPRLSFFWGIGMNFYMEIAKMRAGRRLWAHLIEKMFQPKNSKSLLLRAHCQTS
GWSLTEQDPYNNIVRTAIEAMAAVFGGTQSLHTNSFDEALGLPTVKSARIARNTQIIIQE
ESGIPKVADPWGGSYMMECLTNDVYDAALKLINEIEEMGGMAKAVAEGIPKLRIEECAAR
RQARIDSGSEVIVGVNKYQLEKEDTVEVLAIDNTSVRNRQIEKLKKIKSSRDQALAERCL
AALTECAASGDGNILALAVDASRARCTVGEITDALKKVFGEHKANDRMVSGAYRQEFGES
KEITSAIKRVHKFMEREGRRPRLLVAKMGQDGHDRGAKVIATGFADLGFDVDIGPLFQTP
REVAQQAVDADVHAVGVSTLAAGHKTLVPELIKELNSLGRPDILVMCGGVIPPQDYEFLF
EVGVSNVFGPGTRIPKAAVQVLDDIEKCLEKKQQSV**

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 2274

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pVP16

Synonyms:

Sequencing Primer:

Forward: MBP Forward

Reverse: pQE Reverse

Description:

Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; ampicillin resistance in bacteria; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.

Comments:

TEV protease cleavage site is "partial" and not functional. The TEV cleavage site must be added to the insert by PCR, which allows amino acids encoded by the attB1 site to be cut off after protein expression. Gateway cloning into this vector will remove the Gateway cassette

Size (bp):

7631

Parent Vector:

None

Properties:

Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker

Author Name:

Center for Eukaryotic Structural Genomics

Publications:

PMID: 15750721

Title: Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination

Vector Map:

Vector Sequence:

Sequence:

CCTCGAGAAATCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATAGATTCAATTGTGAGCGGATAACAATTTCACACAGAATTCATTAAAGAGGAGAAATTAACCATGGGACATCACCATCATCACCATCACCATTCAAAAATCGAAGAAGGTAAACTGGTAATCTGGATTAACGGCGATAAAGGCTATAACGGTCTCGCTGAAGTCGGTAAGAAATTCGAGAAAGATACCGGAATTAAAGTCACCGTTGAGCATCCGGATAAACTGGAAGAGAAATTCCCACAGGTTGCGGCAACTGGCGATGGCCCTGACATTATCTTCTGGGCACACGACCGCTTTGGTGGCTACGCTCAATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGGACAAGCTGTATCCGTTTACCTGGGATGCCGTACGTTACAACGGCAAGCTGATTGCTTACCCGATCGCTGTTGAAGCGTTATCGCTGATTTATAACAAAGATCTGCTGCCGAACCCGCCAAAAACCTGGGAAGAGATCCCGGCGCTGGATAAAGAACTGAAAGCGAAAGGTAAGAGCGCGCTGATGTTCAACCTGCAAGAACCGTACTTCACCTGGCCGCTGATTGCTGCTGACGGGGGTTATGCGTTCAAGTATGAAAACGGCAAGTACGACATTAAAGACGTGGGCGTGGATAACGCTGGCGCGAAAGCGGGTCTGACCTTCCTGGTTGACCTGATTAAAAACAAACACATGAATGCAGACACCGATTACTCCATCGCAGAAGCTGCCTTTAATAAAGGCGAAACAGCGATGACCATCAACGGCCCGTGGGCATGGTCCAACATCGACACCAGCAAAGTGAATTATGGTGTAACGGTACTGCCGACCTTCAAGGGTCAACCATCCAAACCGTTCGTTGGCGTGCTGAGCGCAGGTATTAACGCCGCCAGTCCGAACAAAGAGCTGGCAAAAGAGTTCCTCGAAAACTATCTGCTGACTGATGAAGGTCTGGAAGCGGTTAATAAAGACAAACCGCTGGGTGCCGTAGCGCTGAAGTCTTACGAGGAAGAGTTGGCGAAAGATCCACGTATTGCCGCCACTATGGAAAACGCCCAGAAAGGTGAAATCATGCCGAACATCCCGCAGATGTCCGCTTTCTGGTATGCCGTGCGTACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGTCGATGAAGCCCTGAAAGACGCGCAGACTAATTCGAGCTCGAACAACAACAACAATAACAATAACAACAACCTCGGGATCGACGAAAACCTGTACCTCACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTTCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATGGCGCGCCTGTAGGACGTCGACGGTACCATCGATACGCGTTCGAAGCTTAATTAGCTGAGCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAAGCTAGCTTGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATTTTTTTAAGGCAGTTATTGGTGCCCTTAAACGCCTGGGGTAATGACTCTCTAGCTTGAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCCTCTAGATTACGTGCAGTCGATGATAAGCTGTCAAACATGAGAATTGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCGGAATTTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCTAGAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAC

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColE1-type bacterial origin of replication

6471

5789

negative selection marker

ccdB

ccdB death cassette gene (lost in with-insert form)

2643

2852

promoter

T5 promoter

recombination site

AttR1

AttR1 recombination site

1316

1440

recombination site

AttR2

AttR2 recombination site

3020

2896

repressor protein gene

LacI

LacI coding region

4132

5211

selectable marker

CmR

chloramphenicol resistance gene (CmR) (Lost in with-insert form)

1549

2205

selectable marker

AmpR

ampicillin resistance gene

7228

6569

tag

MBP

N-terminal MBP

148

1246

tag

His

N-terminal 8xHis tag

128

145

Gene

Gene Symbol :

MUT

Symbol Nomenclature :

MUT

SYNONYM :

MCM

Designation :

methylmalonyl Coenzyme A mutase|methylmalonyl-CoA isomerase|methylmalonyl-CoA mutase, mitochondrial

Full Nomenclature :

methylmalonyl CoA mutase

GENEID :

4594

HGNC :

7526

MIM :

609058

Vega :

OTTHUMG00000014814

Target GenBank:

BC016282

Reference Sequence Alignment

  

HsCD00084295      ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCTTAAGAGCTAAG
NM_000255.3       ---------------------------------------------ATGTTAAGAGCTAAG
                                                               :  ************

HsCD00084295      AATCAGCTTTTTTTACTTTCACCTCATTACCTGAGGCAGGTAAAAGAATCATCAGGCTCC
NM_000255.3       AATCAGCTTTTTTTACTTTCACCTCATTACCTGAGGCAGGTAAAAGAATCATCAGGCTCC
                  ************************************************************

HsCD00084295      AGGCTCATACAGCAACGACTTCTACACCAGCAACAGCCCCTTCACCCAGAATGGGCTGCC
NM_000255.3       AGGCTCATACAGCAACGACTTCTACACCAGCAACAGCCCCTTCACCCAGAATGGGCTGCC
                  ************************************************************

HsCD00084295      CTGGCTAAAAAGCAGCTGAAAGGCAAAAACCCAGAAGACCTAATATGGCACACCCCGGAA
NM_000255.3       CTGGCTAAAAAGCAGCTGAAAGGCAAAAACCCAGAAGACCTAATATGGCACACCCCGGAA
                  ************************************************************

HsCD00084295      GGGATCTCTATAAAACCCTTGTATTCCAAGAGAGATACTATGGACTTACCTGAAGAACTT
NM_000255.3       GGGATCTCTATAAAACCCTTGTATTCCAAGAGAGATACTATGGACTTACCTGAAGAACTT
                  ************************************************************

HsCD00084295      CCAGGAGTGAAGCCATTCACACGTGGACCATATCCTACCATGTATACCTTTAGGCCCTGG
NM_000255.3       CCAGGAGTGAAGCCATTCACACGTGGACCATATCCTACCATGTATACCTTTAGGCCCTGG
                  ************************************************************

HsCD00084295      ACCATCCGCCAGTATGCTGGTTTTAGTACTGTGGAAGAAAGCAATAAGTTCTATAAGGAC
NM_000255.3       ACCATCCGCCAGTATGCTGGTTTTAGTACTGTGGAAGAAAGCAATAAGTTCTATAAGGAC
                  ************************************************************

HsCD00084295      AACATTAAGGCTGGTCAGCAGGGATTATCAGTTGCCTTTGATCTGGCGACACATCGTGGC
NM_000255.3       AACATTAAGGCTGGTCAGCAGGGATTATCAGTTGCCTTTGATCTGGCGACACATCGTGGC
                  ************************************************************

HsCD00084295      TATGATTCAGACAACCCTCGAGTTCGTGGTGATGTTGGAATGGCTGGAGTTGCTATTGAC
NM_000255.3       TATGATTCAGACAACCCTCGAGTTCGTGGTGATGTTGGAATGGCTGGAGTTGCTATTGAC
                  ************************************************************

HsCD00084295      ACTGTGGAAGATACCAAAATTCTTTTTGATGGAATTCCTTTAGAAAAAATGTCAGTTTCC
NM_000255.3       ACTGTGGAAGATACCAAAATTCTTTTTGATGGAATTCCTTTAGAAAAAATGTCAGTTTCC
                  ************************************************************

HsCD00084295      ATGACTATGAATGGAGCAGTTATTCCAGTTCTTGCAAATTTTATAGTAACTGGAGAAGAA
NM_000255.3       ATGACTATGAATGGAGCAGTTATTCCAGTTCTTGCAAATTTTATAGTAACTGGAGAAGAA
                  ************************************************************

HsCD00084295      CAAGGTGTACCTAAAGAGAAACTTACTGGTACCATCCAAAATGATATACTAAAGGAATTT
NM_000255.3       CAAGGTGTACCTAAAGAGAAGCTTACTGGTACCATCCAAAATGATATACTAAAGGAATTT
                  ********************.***************************************

HsCD00084295      ATGGTTCGAAATACATACATTTTTCCTCCAGAACCATCCATGAAAATTATTGCTGACATA
NM_000255.3       ATGGTTCGAAATACATACATTTTTCCTCCAGAACCATCCATGAAAATTATTGCTGACATA
                  ************************************************************

HsCD00084295      TTTGAATATACAGCAAAGCACATGCCAAAATTTAATTCAATTTCAATTAGTGGATACCAT
NM_000255.3       TTTGAATATACAGCAAAGCACATGCCAAAATTTAATTCAATTTCAATTAGTGGATACCAT
                  ************************************************************

HsCD00084295      ATGCAGGAAGCAGGGGCTGATGCCATTCTGGAGCTGGCCTATACTTTAGCAGATGGATTG
NM_000255.3       ATGCAGGAAGCAGGGGCTGATGCCATTCTGGAGCTGGCCTATACTTTAGCAGATGGATTG
                  ************************************************************

HsCD00084295      GAGTACTCTAGAACTGGACTCCAGGCTGGCCTGACAATTGATGAATTTGCACCAAGGTTG
NM_000255.3       GAGTACTCTAGAACTGGACTCCAGGCTGGCCTGACAATTGATGAATTTGCACCAAGGTTG
                  ************************************************************

HsCD00084295      TCTTTCTTCTGGGGAATTGGAATGAATTTCTATATGGAAATAGCAAAGATGAGAGCTGGT
NM_000255.3       TCTTTCTTCTGGGGAATTGGAATGAATTTCTATATGGAAATAGCAAAGATGAGAGCTGGT
                  ************************************************************

HsCD00084295      AGAAGACTCTGGGCTCACTTAATAGAGAAAATGTTTCAGCCTAAAAACTCAAAATCTCTT
NM_000255.3       AGAAGACTCTGGGCTCACTTAATAGAGAAAATGTTTCAGCCTAAAAACTCAAAATCTCTT
                  ************************************************************

HsCD00084295      CTTCTAAGAGCACACTGTCAGACATCTGGATGGTCACTTACTGAGCAGGATCCCTACAAT
NM_000255.3       CTTCTAAGAGCACACTGTCAGACATCTGGATGGTCACTTACTGAGCAGGATCCCTACAAT
                  ************************************************************

HsCD00084295      AATATTGTCCGTACTGCAATAGAAGCAATGGCAGCAGTATTTGGAGGGACTCAGTCTTTG
NM_000255.3       AATATTGTCCGTACTGCAATAGAAGCAATGGCAGCAGTATTTGGAGGGACTCAGTCTTTG
                  ************************************************************

HsCD00084295      CACACAAATTCTTTTGATGAAGCTTTGGGTTTGCCAACTGTGAAAAGTGCTCGAATTGCC
NM_000255.3       CACACAAATTCTTTTGATGAAGCTTTGGGTTTGCCAACTGTGAAAAGTGCTCGAATTGCC
                  ************************************************************

HsCD00084295      AGGAACACACAAATCATCATTCAAGAAGAATCTGGGATTCCCAAAGTGGCTGATCCTTGG
NM_000255.3       AGGAACACACAAATCATCATTCAAGAAGAATCTGGGATTCCCAAAGTGGCTGATCCTTGG
                  ************************************************************

HsCD00084295      GGAGGTTCTTACATGATGGAATGTCTCACAAATGATGTTTATGATGCTGCTTTAAAGCTC
NM_000255.3       GGAGGTTCTTACATGATGGAATGTCTCACAAATGATGTTTATGATGCTGCTTTAAAGCTC
                  ************************************************************

HsCD00084295      ATTAATGAAATTGAAGAAATGGGTGGAATGGCCAAAGCTGTAGCTGAGGGAATACCTAAA
NM_000255.3       ATTAATGAAATTGAAGAAATGGGTGGAATGGCCAAAGCTGTAGCTGAGGGAATACCTAAA
                  ************************************************************

HsCD00084295      CTTCGAATTGAAGAATGTGCTGCCCGAAGACAAGCTAGAATAGATTCTGGTTCTGAAGTA
NM_000255.3       CTTCGAATTGAAGAATGTGCTGCCCGAAGACAAGCTAGAATAGATTCTGGTTCTGAAGTA
                  ************************************************************

HsCD00084295      ATTGTTGGAGTAAATAAGTACCAGTTGGAAAAAGAAGACACTGTAGAAGTTCTGGCAATT
NM_000255.3       ATTGTTGGAGTAAATAAGTACCAGTTGGAAAAAGAAGACGCTGTAGAAGTTCTGGCAATT
                  ***************************************.********************

HsCD00084295      GATAATACTTCAGTGCGAAACAGGCAGATTGAAAAACTTAAGAAGATCAAATCCAGCAGG
NM_000255.3       GATAATACTTCAGTGCGAAACAGGCAGATTGAAAAACTTAAGAAGATCAAATCCAGCAGG
                  ************************************************************

HsCD00084295      GATCAAGCTTTGGCTGAACGTTGTCTTGCTGCACTAACCGAATGTGCTGCTAGCGGAGAT
NM_000255.3       GATCAAGCTTTGGCTGAACGTTGTCTTGCTGCACTAACCGAATGTGCTGCTAGCGGAGAT
                  ************************************************************

HsCD00084295      GGAAATATCCTGGCTCTTGCAGTGGATGCATCTCGGGCAAGATGTACAGTGGGAGAAATC
NM_000255.3       GGAAATATCCTGGCTCTTGCAGTGGATGCATCTCGGGCAAGATGTACAGTGGGAGAAATC
                  ************************************************************

HsCD00084295      ACAGATGCCCTGAAAAAGGTATTTGGTGAACATAAAGCGAATGATCGAATGGTGAGTGGA
NM_000255.3       ACAGATGCCCTGAAAAAGGTATTTGGTGAACATAAAGCGAATGATCGAATGGTGAGTGGA
                  ************************************************************

HsCD00084295      GCATATCGCCAGGAATTTGGAGAAAGTAAAGAGATAACATCTGCTATCAAGAGGGTTCAT
NM_000255.3       GCATATCGCCAGGAATTTGGAGAAAGTAAAGAGATAACATCTGCTATCAAGAGGGTTCAT
                  ************************************************************

HsCD00084295      AAATTCATGGAACGTGAAGGTCGCAGACCTCGTCTTCTTGTAGCAAAAATGGGACAAGAT
NM_000255.3       AAATTCATGGAACGTGAAGGTCGCAGACCTCGTCTTCTTGTAGCAAAAATGGGACAAGAT
                  ************************************************************

HsCD00084295      GGCCATGACAGAGGAGCAAAAGTTATTGCTACAGGATTTGCTGATCTTGGTTTTGATGTG
NM_000255.3       GGCCATGACAGAGGAGCAAAAGTTATTGCTACAGGATTTGCTGATCTTGGTTTTGATGTG
                  ************************************************************

HsCD00084295      GACATAGGCCCTCTTTTCCAGACTCCTCGTGAAGTGGCCCAGCAGGCTGTGGATGCGGAT
NM_000255.3       GACATAGGCCCTCTTTTCCAGACTCCTCGTGAAGTGGCCCAGCAGGCTGTGGATGCGGAT
                  ************************************************************

HsCD00084295      GTGCATGCTGTGGGCGTAAGCACCCTCGCTGCTGGTCATAAAACCCTAGTTCCTGAACTC
NM_000255.3       GTGCATGCTGTGGGCATAAGCACCCTCGCTGCTGGTCATAAAACCCTAGTTCCTGAACTC
                  ***************.********************************************

HsCD00084295      ATCAAAGAACTTAACTCCCTTGGACGGCCAGATATTCTTGTCATGTGTGGAGGGGTGATA
NM_000255.3       ATCAAAGAACTTAACTCCCTTGGACGGCCAGATATTCTTGTCATGTGTGGAGGGGTGATA
                  ************************************************************

HsCD00084295      CCACCTCAGGATTATGAATTTCTGTTTGAAGTTGGTGTTTCCAATGTATTTGGTCCTGGG
NM_000255.3       CCACCTCAGGATTATGAATTTCTGTTTGAAGTTGGTGTTTCCAATGTATTTGGTCCTGGG
                  ************************************************************

HsCD00084295      ACTCGAATTCCAAAGGCTGCCGTTCAGGTGCTTGATGATATTGAGAAGTGTTTGGAAAAG
NM_000255.3       ACTCGAATTCCAAAGGCTGCCGTTCAGGTGCTTGATGATATTGAGAAGTGTTTGGAAAAG
                  ************************************************************

HsCD00084295      AAGCAGCAATCTGTATAATAGGACCCAGCTTTCTTGTACAAAGTGGT
NM_000255.3       AAGCAGCAATCTGTATAA-----------------------------
                  ******************

Pathways

Panther :

Methylmalonyl pathway

Panther :

Succinate to proprionate conversion

Reactome :

Cobalamin (Cbl, vitamin B12) transport and metabolism

Reactome :

Defective AMN causes hereditary megaloblastic anemia 1

Reactome :

Defective BTD causes biotidinase deficiency

Reactome :

Defective CD320 causes methylmalonic aciduria

Reactome :

Defective CUBN causes hereditary megaloblastic anemia 1

Reactome :

Defective GIF causes intrinsic factor deficiency

Reactome :

Defective HLCS causes multiple carboxylase deficiency

Reactome :

Defective LMBRD1 causes methylmalonic aciduria and homocystinuria type cblF

Reactome :

Defective MMAA causes methylmalonic aciduria type cblA

Reactome :

Defective MMAB causes methylmalonic aciduria type cblB

Reactome :

Defective MMACHC causes methylmalonic aciduria and homocystinuria type cblC

Reactome :

Defective MMADHC causes methylmalonic aciduria and homocystinuria type cblD

Reactome :

Defective MTR causes methylmalonic aciduria and homocystinuria type cblG

Reactome :

Defective MTRR causes methylmalonic aciduria and homocystinuria type cblE

Reactome :

Defective MUT causes methylmalonic aciduria mut type

Reactome :

Defective TCN2 causes hereditary megaloblastic anemia

Reactome :

Defects in biotin (Btn) metabolism

Reactome :

Defects in cobalamin (B12) metabolism

Reactome :

Defects in vitamin and cofactor metabolism

Reactome :

Disease

Reactome :

Fatty acid, triacylglycerol, and ketone body metabolism

Reactome :

Metabolism

Reactome :

Metabolism of lipids and lipoproteins

Reactome :

Metabolism of vitamins and cofactors

Reactome :

Metabolism of water-soluble vitamins and cofactors

Reactome :

Mitochondrial Fatty Acid Beta-Oxidation

Reactome :

Propionyl-CoA catabolism

WikiPathway :

Vitamin B12 Metabolism

Protein

Structural Annotation Wiki :

TOPSAN

UniProt :

P22033

UniProt :

B2R6K1

HPRD :

02014

Clone

Cloning Information :

273318

HIP Master Clone ID :

203186

Original Clone ID :

1265.1.43_GO.41458

Experimental

TargetTrack - Experimental data :

GO.41458

Organism

Species Specific ID:

4594

Genome

Chromosome :

Map Location :

6p12.3

Ensembl :

ENSG00000146085

Reagents

Labome :

MUT-antibody