Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
1191.1.35_GO.29756
Keyword:
None
Species:
Arabidopsis thaliana Columbia
Type:
cDNA
Vector Name:
pVP16
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
AtCD00084300
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 2241nts
Open reading frame: 51 to 2291

GGATCGACGAAAACCTGTACCTCACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCGGTACTTCGGTGCAAGTGACTCCTCTCTGCGGAGTTTACAACGAGAATC
CTCTCTCCTACTTAGTCTCCATTGATGGTTTCAACTTCCTCATCGACTGTGGTTGGAACG
ATCTCTTCGACACATCGCTCCTCGAACCTCTCTCCAGGGTTGCTTCTACTATAGATGCAG
TTTTGCTTTCTCATCCAGATACACTTCACATTGGTGCTCTTCCTTATGCTATGAAGCAGC
TTGGACTCTCCGCTCCTGTTTATGCTACTGAGCCTGTTCATAGATTAGGTCTCCTTACAA
TGTATGATCAGTTTTTGTCCAGGAAGCAAGTATCCGACTTTGATCTGTTTACACTGGATG
ACATAGATTCTGCTTTCCAGAATGTGATCAGATTGACTTACTCTCAAAATTACCATCTTT
CTGGAAAGGGAGAGGGTATTGTAATTGCTCCTCATGTTGCTGGACACATGCTGGGAGGTA
GCATCTGGAGGATAACAAAGGATGGGGAGGATGTTATATATGCTGTTGACTACAATCATC
GGAAAGAAAGGCATTTGAATGGAACTGTTTTACAGTCTTTTGTTCGGCCTGCTGTTCTGA
TAACCGATGCATATCATGCTCTTTATACCAATCAAACCGCAAGACAGCAAAGGGACAAAG
AATTTCTAGATACCATTTCAAAACATCTTGAAGTTGGAGGAAATGTTCTATTGCCAGTAG
ACACTGCGGGTCGTGTCCTGGAACTTCTCTTGATACTTGAACAGCATTGGTCACAAAGAG
GTTTCAGCTTTCCCATTTATTTTCTCACGTACGTGTCATCTAGCACAATTGACTATGTTA
AGAGTTTCCTCGAGTGGATGAGTGACTCCATTTCAAAGTCCTTTGAGACTTCACGTGATA
ATGCCTTTCTATTGAGGCATGTCACTCTGTTGATAAACAAGACTGATCTGGATAATGCTC
CACCTGGTCCAAAGGTTGTTCTTGCTTCCATGGCTAGTCTCGAAGCCGGTTTTGCTCGAG
AGATATTCGTGGAGTGGGCAAATGATCCCAGAAATTTAGTCCTCTTTACTGAAACAGGCC
AGTTTGGCACTTTAGCTCGTATGCTTCAGTCAGCCCCACCTCCAAAATTTGTTAAAGTCA
CCATGTCTAAGAGGGTTCCTTTGGCTGGGGAAGAGTTAATTGCATATGAGGAAGAGCAAA
ACAGACTGAAAAGGGAAGAAGCTTTGCGAGCTAGCCTCGTTAAAGAGGAGGAAACAAAAG
CTTCCCATGGATCCGATGATAATTCAAGTGAACCTATGATCATAGATACCAAGACTACTC
ACGATGTTATTGGTTCTCACGGGCCTGCATATAAAGATATATTGATTGATGGATTTGTTC
CCCCATCCAGCAGCGTAGCTCCAATGTTCCCATATTATGATAACACATCTGAATGGGACG
ACTTTGGGGAGATTATTAATCCAGATGACTATGTGATCAAGGATGAAGACATGGACCGAG
GAGCAATGCATAACGGAGGTGATGTGGACGGAAGGCTTGATGAGGCAACTGCTAGTCTCA
TGCTTGATACAAGACCTTCGAAAGTCATGTCCAATGAGCTCATTGTGACTGTTAGTTGTT
CACTTGTTAAAATGGACTATGAAGGTCGGTCAGATGGCCGCTCAATCAAGTCAATGATTG
CGCATGTTTCTCCTTTAAAACTTGTTTTGGTGCACGCGATAGCTGAGGCTACAGAGCATT
TGAAGCAACACTGCTTGAACAACATCTGTCCACACGTGTATGCTCCTCAAATAGAGGAAA
CGGTCGATGTGACTTCTGATTTATGTGCTTACAAGGTCCAACTTTCTGAAAAGCTGATGA
GCAATGTGATCTTCAAGAAGCTGGGAGATTCAGAAGTAGCTTGGGTGGATTCCGAAGTAG
GGAAGACAGAGAGGGACATGAGGTCTCTACTACCGATGCCAGGTGCTGCTTCGCCACACA
AACCTGTTCTAGTAGGTGATCTGAAAATCGCAGACTTCAAGCAGTTTCTGTCGAGCAAGG
GTGTTCAGGTAGAATTTGCAGGTGGAGGAGCTTTACGTTGTGGTGAATATGTCACTCTAC
GAAAGGTTGGACCGACGGGTCAAAAGGGAGGAGCATCGGGTCCACAGCAAATTCTGATAG
AAGGACCGTTGTGTGAAGACTATTACAAAATCAGGGATTATCTCTATTCTCAGTTCTACC
TCCTCTGATAGGACCCAGCTTTCTTGTACAAAGTGGTGATGGCGCGCCTGTAGGACGTCG
A

Protein sequence predicted based on nucleotide sequence

ENLYFQSGTSVQVTPLCGVYNENPLSYLVSIDGFNFLIDCGWNDLFDTSLLEPLSRVAST
IDAVLLSHPDTLHIGALPYAMKQLGLSAPVYATEPVHRLGLLTMYDQFLSRKQVSDFDLF
TLDDIDSAFQNVIRLTYSQNYHLSGKGEGIVIAPHVAGHMLGGSIWRITKDGEDVIYAVD
YNHRKERHLNGTVLQSFVRPAVLITDAYHALYTNQTARQQRDKEFLDTISKHLEVGGNVL
LPVDTAGRVLELLLILEQHWSQRGFSFPIYFLTYVSSSTIDYVKSFLEWMSDSISKSFET
SRDNAFLLRHVTLLINKTDLDNAPPGPKVVLASMASLEAGFAREIFVEWANDPRNLVLFT
ETGQFGTLARMLQSAPPPKFVKVTMSKRVPLAGEELIAYEEEQNRLKREEALRASLVKEE
ETKASHGSDDNSSEPMIIDTKTTHDVIGSHGPAYKDILIDGFVPPSSSVAPMFPYYDNTS
EWDDFGEIINPDDYVIKDEDMDRGAMHNGGDVDGRLDEATASLMLDTRPSKVMSNELIVT
VSCSLVKMDYEGRSDGRSIKSMIAHVSPLKLVLVHAIAEATEHLKQHCLNNICPHVYAPQ
IEETVDVTSDLCAYKVQLSEKLMSNVIFKKLGDSEVAWVDSEVGKTERDMRSLLPMPGAA
SPHKPVLVGDLKIADFKQFLSSKGVQVEFAGGGALRCGEYVTLRKVGPTGQKGGASGPQQ
ILIEGPLCEDYYKIRDYLYSQFYLL**

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 2241
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable