Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
1191.1.26_GO.19503
Keyword:
None
Species:
Arabidopsis thaliana Columbia
Type:
cDNA
Vector Name:
pVP16
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
AtCD00084305
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1770nts
Open reading frame: 28 to 1797

ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCAGAAATTTCAGT
GTCTTCGAGAGATTCTCTAAAGCTTTTAAAGATCATCCTTCTCTCACCAGGATTCTCGTC
GTCTCCACGATCAGTGGTGGTGGACTTATAGCGTATTCTGAGGCAAACGCATCTTATGGA
GCAAATGGTGGTGCTGTTGTTGAAACTGGAACCAAGAAGAAGAAGGTGGTGCTGCTCGGA
ACTGGATGGGCTGGAACTAGTTTCTTGAAGAATTTGAACAATTCTCAATATGAGGTTCAG
ATTATATCGCCTCGGAACTACTTTGCTTTCACTCCTTTACTACCTAGTGTTACTTGTGGA
ACTGTTGAAGCTCGTAGTGTTGTTGAGCCTATTCGAAACATTGGAAGAAAGAATGTTGAT
ACTTCTTACTTGGAGGCAGAATGTTTCAAAATTGATCCAGCTAGCAAGAAGGTTTACTGC
CGATCCAAACAAGGCCTTAGTTCAAATGGAAAGAAAGAATTTTCTGTTGACTATGACTAC
CTTGTAATTGCTACTGGAGCTCAGTCTAACACCTTTAACATACCCGGAGTGGAGGAGAAC
TGTCATTTTCTGAAAGAGGTTGAAGATGCTCAGAGAATCCGTAAAACTGTAATTGATTCC
TTTGAGAAGGCAAGTCTACCAGAACTAAGCGATGAAGAGAGAAAGAGAATCCTGCATTTT
GTGGTTGTTGGTGGTGGGCCAACAGGTGTTGAGTTTGCCGCTGAGCTACATGATTTTGTT
ACTGAGGATCTAGTCAGTCTCTATCCTAGAGCCAAGGGTTCAGTGCGAATCACGCTTCTG
GAGGCCGCAGACCACATCCTGACCATGTTTGACAAGAGAATCACTGAGTTTGCCGAAGAA
AAGTTTAGCAGAGATGGAATTGATGTGAAACTTGGCTCGATGGTAACTAAAGTGAATGAG
AAAGATATATCTGCTAAAACCAAAGGAGGAGAGGTTTCCTCGATTCCTTATGGAATGATT
GTCTGGTCAACTGGTATTGGAACTCGTCCTGTGATAAAAGATTTTATGAAACAAATTGGT
CAGGGTAATAGACGAGCTCTGGCAACTGATGAATGGCTTCGCGTAGAAGGAACTGATAAT
ATATATGCCCTTGGTGATTGCGCAACCATTAACCAGCGCAAAGTCATGGAAGATGTTTCT
GCTATTTTTAGCAAAGCAGACAAAGACAAGTCTGGGACACTGACTCTCAAAGAGTTCCAA
GAAGCAATGGATGACATTTGTGTTAGATACCCTCAAGTGGAGCTCTACTTGAAGAGTAAA
CGTATGCGTGGCATTGCAGATCTTCTTAAAGAAGCCGAAACTGATGATGTATCTAAGAAT
AACATTGAACTGAAGATCGAAGAGTTCAAATCGGCTCTATCTCAGGTTGACTCACAAGTG
AAGTTTCTTCCAGCTACTGCACAGGTTGCTGCGCAGCAAGGTGCTTACCTAGCTAAATGC
TTTGACCGTATGGAAGAGTGTGAGAAGAGTCCAGAAGGTCCCATTAGGATGAGAGGAGAA
GGTCGTCACCGTTTCCGCCCCTTCAGGTATAGGCATCTGGGACAATTTGCACCACTGGGA
GGGGAGCAAACGGCTGCACAACTTCCAGGAGATTGGGTTTCTATTGGACACAGCAGCCAG
TGGCTCTGGTACTCCGTCTACGCCAGTAAGCAAGTGAGCTGGCGTACGAGAGTGCTTGTG
GTTTCAGATTGGATGAGGCGTTTCATCTTTGGTAGAGATTCCAGTAGCATCTGATAGGAC
CCAGCTTTCTTGTACAAAGTGGT

Protein sequence predicted based on nucleotide sequence

ENLYFQSRNFSVFERFSKAFKDHPSLTRILVVSTISGGGLIAYSEANASYGANGGAVVET
GTKKKKVVLLGTGWAGTSFLKNLNNSQYEVQIISPRNYFAFTPLLPSVTCGTVEARSVVE
PIRNIGRKNVDTSYLEAECFKIDPASKKVYCRSKQGLSSNGKKEFSVDYDYLVIATGAQS
NTFNIPGVEENCHFLKEVEDAQRIRKTVIDSFEKASLPELSDEERKRILHFVVVGGGPTG
VEFAAELHDFVTEDLVSLYPRAKGSVRITLLEAADHILTMFDKRITEFAEEKFSRDGIDV
KLGSMVTKVNEKDISAKTKGGEVSSIPYGMIVWSTGIGTRPVIKDFMKQIGQGNRRALAT
DEWLRVEGTDNIYALGDCATINQRKVMEDVSAIFSKADKDKSGTLTLKEFQEAMDDICVR
YPQVELYLKSKRMRGIADLLKEAETDDVSKNNIELKIEEFKSALSQVDSQVKFLPATAQV
AAQQGAYLAKCFDRMEECEKSPEGPIRMRGEGRHRFRPFRYRHLGQFAPLGGEQTAAQLP
GDWVSIGHSSQWLWYSVYASKQVSWRTRVLVVSDWMRRFIFGRDSSSI**

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1770
Mutations:
None
Discrepancy:
a1608t,A536A;
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable