Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
1191.1.37_GO.30907
Keyword:
None
Species:
Arabidopsis thaliana Columbia
Type:
cDNA
Vector Name:
pVP16
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
AtCD00084306
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 2076nts
Open reading frame: 28 to 2103

ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCGCGATTATTACT
ACTACTACTGTTCGTTTCACTGATGGAACCTCTCCCACCTTCTTCTCCTCAGCTTCGACA
AAGGCTTATAATCTCCATTTTCTCTACTCGAATTCAACCCAACGACTTACGAATCCGAAA
TTCGGAATCGGCGGGAAGTTGAAGGTGACGGTGAATCCGTATTCGTATACAGAGGAAGTA
CGGCCTGAGGAACGGAAGAGTTTGACGGATTTTTTAACGGAAGCTGGAGATTTCGTTAAT
TCAGACGGCGGAGATGGTGGTCCGCCACGGTGGTTCTCACCGTTGGAATGTGGCGCACGT
GCTCCTGAATCTCCTCTTCTTCTCTACTTACCTGGGATCGATGGAACTGGATTAGGGCTC
ATTCGCCAGCATAAGAGGCTTGGAGAGATATTTGACATATGGTGCCTTCACTTTCCAGTA
AAAGATCGTACTCCTGCTCGAGATATTGGGAAGCTCATTGAGAAGACAGTTAGGTCAGAG
CACTACCGTTTCCCAAATAGACCCATTTATATAGTTGGAGAATCTATTGGAGCTTCTCTT
GCTCTGGATGTTGCAGCCAGTAACCCTGACATTGATCTTGTCTTGATTCTGGCTAATCCA
GTCACACGTTTTACCAACTTAATGTTGCAACCTGTATTGGCCCTACTGGAAATTTTGCCT
GACGGAGTTCCCGGCTTGATAACAGAGAATTTTGGGTTTTACCAAGAAATGTTCGAGACT
ATGCTCAATGAAAATGATGCCGCGCAGATGGGTAGAGGGCTATTAGGAGACTTCTTTGCA
ACTTCATCTAATCTGCCTACTCTGATTAGAATCTTTCCCAAGGACACACTTCTATGGAAG
CTTCAATTGCTTAAGTCTGCTTCAGCGTCTGCTAATTCTCAGATGGACACAGTCAACGCC
CAAACACTGATACTTCTGAGTGGACGTGATCAATGGTTAATGAACAAGGAAGACATTGAA
AGACTCCGTGGTGCATTGCCAAGATGTGAAGTTCGTGAGCTTGAGAATAATGGACAGTTC
CTCTTCTTGGAGGATGGAGTAGATCTGGTGAGTATCATCAAGCGTGCGTATTATTATCGC
CGTGGGAAGTCACTTGATTACATTTCGGATTACATTCTGCCTACCCCATTTGAGTTTAAA
GAGTATGAAGAATCACAAAGATTGCTAACTGCTGTTACCTCCCCAGTCTTTCTTTCAACT
CTAAAGAATGGTGCAGTGGTAAGATCGCTTGCAGGAATACCTTCAGAGGGACCGGTTCTG
TATGTTGGCAATCACATGTTGCTTGGTATGGAGTTGCATGCAATAGCACTTCATTTTTTG
AAAGAAAGGAACATTCTATTGCGAGGACTGGCACATCCATTGATGTTTACCAAAAAAACT
GGCTCAAAACTCCCTGACATGCAGCTGTACGACTTATTTAGGATTATAGGCGCAGTTCCC
GTCTCGGGAATGAATTTCTACAAACTACTTCGTTCAAAGGCTCACGTGGCTTTGTACCCT
GGGGGTGTTCGTGAAGCTTTGCACAGAAAGGGTGAAGAATACAAGTTATTTTGGCCAGAA
CATTCGGAGTTTGTAAGGATAGCATCTAAATTTGGAGCAAAAATCATTCCTTTTGGAGTT
GTTGGAGAAGATGATCTTTGTGAAATGGTTTTAGATTATGATGATCAAATGAAGATCCCT
TTCTTGAAGAATCTTATAGAAGAGATAACACAAGACTCTGTTAACTTGAGGAACGATGAA
GAAGGCGAATTGGGAAAACAAGATTTACATCTACCTGGAATAGTTCCAAAGATCCCGGGA
CGGTTTTACGCATACTTTGGGAAACCAATAGACACAGAAGGTAGAGAGAAAGAGCTAAAC
AATAAAGAGAAAGCTCATGAGGTTTACTTGCAGGTCAAGTCTGAGGTAGAAAGATGTATG
AACTATTTGAAAATCAAAAGAGAAACTGATCCTTACAGAAACATTTTGCCGAGGTCCCTC
TATTACCTCACTCATGGTTTCTCTTCCCAAATCCCAACCTTCGATCTCCGAAATCATTAA
TAGGACCCAGCTTTCTTGTACAAAGTGGT

Protein sequence predicted based on nucleotide sequence

ENLYFQSAIITTTTVRFTDGTSPTFFSSASTKAYNLHFLYSNSTQRLTNPKFGIGGKLKV
TVNPYSYTEEVRPEERKSLTDFLTEAGDFVNSDGGDGGPPRWFSPLECGARAPESPLLLY
LPGIDGTGLGLIRQHKRLGEIFDIWCLHFPVKDRTPARDIGKLIEKTVRSEHYRFPNRPI
YIVGESIGASLALDVAASNPDIDLVLILANPVTRFTNLMLQPVLALLEILPDGVPGLITE
NFGFYQEMFETMLNENDAAQMGRGLLGDFFATSSNLPTLIRIFPKDTLLWKLQLLKSASA
SANSQMDTVNAQTLILLSGRDQWLMNKEDIERLRGALPRCEVRELENNGQFLFLEDGVDL
VSIIKRAYYYRRGKSLDYISDYILPTPFEFKEYEESQRLLTAVTSPVFLSTLKNGAVVRS
LAGIPSEGPVLYVGNHMLLGMELHAIALHFLKERNILLRGLAHPLMFTKKTGSKLPDMQL
YDLFRIIGAVPVSGMNFYKLLRSKAHVALYPGGVREALHRKGEEYKLFWPEHSEFVRIAS
KFGAKIIPFGVVGEDDLCEMVLDYDDQMKIPFLKNLIEEITQDSVNLRNDEEGELGKQDL
HLPGIVPKIPGRFYAYFGKPIDTEGREKELNNKEKAHEVYLQVKSEVERCMNYLKIKRET
DPYRNILPRSLYYLTHGFSSQIPTFDLRNH**

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 2076
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable