DNASU Plasmid | RNPC3 (Homo sapiens) in pVP16

Explanation of Terms

Gene:

Gene Symbol: RNPC3

Gene Name: RNA-binding region (RNP1, RRM) containing 3

Original Clone ID:

1192.1.70_GO.36706

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pVP16

Format:

CLOSED

Source:

Center for Eukaryotic Structural Genomics

Description:

None

Comments:

His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics

HsCD00084367

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 807nts

Open reading frame: 28 to 834

Reference Sequence Alignment

ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCAACAAATTAATG
GAACTAGCAAATCTTCAGCCCAAAAGACCTAAAACAATAAAGCAGCGCCATGTGAGAAAA
AAGAGAAAAATAAAGGATATGTTGAATACACCTTTGTGTCCTTCACACAGCAGTTTACAT
CCAGTGCTGTTACCTTCAGATGTATTTGACCAACCACAACCTGTAGGTAACAAAAGAATT
GAATTCCATATATCTACCGACATGCCAGCTGCATTTAAGAAAGATTTAGAAAAGGAACAA
AATTGTGAGGAAAAAAATCATGATTTACCTGCTACTGAAGTTGATGCATCCAATATAGGA
TTTGGAAAAATCTTCCCCAAACCTAATTTGGACATCACAGAGGAGATTAAAGAAGACTCT
GATGAAATGCCTTCAGAATGTATTTCTAGAAGGGAATTGGAAAAGGGCAGAATTTCTAGA
GAAGAAATGGAAACACTTTCAGTTTTCAGAAGTTATGAACCGGGTGAACCAAACTGTAGA
ATTTATGTAAAGAATTTAGCTAAACATGTTCAAGAAAAGGACCTTAAATATATTTTTGGA
AGATATGTTGACTTTTCATCAGAAACACAGCGGATCATGTTTGATATACGTTTGATGAAA
GAAGGTCGTATGAAAGGACAAGCTTTCATTGGACTTCCTAATGAAAAAGCAGCAGCAAAA
GCCTTAAAGGAAGCTAATGGATATGTGCTTTTTGGAAAACCCATGGTGGTTCAGTTTGCT
CGATCTGCTAGACCAAAACAAGATCCTAAGGAAGGAAAAAGAAAGTGTTAATAGGACCCA
GCTTTCTTGTACAAAGTGGT

Protein sequence predicted based on nucleotide sequence

ENLYFQSNKLMELANLQPKRPKTIKQRHVRKKRKIKDMLNTPLCPSHSSLHPVLLPSDVF
DQPQPVGNKRIEFHISTDMPAAFKKDLEKEQNCEEKNHDLPATEVDASNIGFGKIFPKPN
LDITEEIKEDSDEMPSECISRRELEKGRISREEMETLSVFRSYEPGEPNCRIYVKNLAKH
VQEKDLKYIFGRYVDFSSETQRIMFDIRLMKEGRMKGQAFIGLPNEKAAAKALKEANGYV
LFGKPMVVQFARSARPKQDPKEGKRKC**

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 807

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pVP16

Synonyms:

Sequencing Primer:

Forward: MBP Forward

Reverse: pQE Reverse

Description:

Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; ampicillin resistance in bacteria; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.

Comments:

TEV protease cleavage site is "partial" and not functional. The TEV cleavage site must be added to the insert by PCR, which allows amino acids encoded by the attB1 site to be cut off after protein expression. Gateway cloning into this vector will remove the Gateway cassette

Size (bp):

7631

Parent Vector:

None

Properties:

Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker

Author Name:

Center for Eukaryotic Structural Genomics

Publications:

PMID: 15750721

Title: Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination

Vector Map:

Vector Sequence:

Sequence:

CCTCGAGAAATCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATAGATTCAATTGTGAGCGGATAACAATTTCACACAGAATTCATTAAAGAGGAGAAATTAACCATGGGACATCACCATCATCACCATCACCATTCAAAAATCGAAGAAGGTAAACTGGTAATCTGGATTAACGGCGATAAAGGCTATAACGGTCTCGCTGAAGTCGGTAAGAAATTCGAGAAAGATACCGGAATTAAAGTCACCGTTGAGCATCCGGATAAACTGGAAGAGAAATTCCCACAGGTTGCGGCAACTGGCGATGGCCCTGACATTATCTTCTGGGCACACGACCGCTTTGGTGGCTACGCTCAATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGGACAAGCTGTATCCGTTTACCTGGGATGCCGTACGTTACAACGGCAAGCTGATTGCTTACCCGATCGCTGTTGAAGCGTTATCGCTGATTTATAACAAAGATCTGCTGCCGAACCCGCCAAAAACCTGGGAAGAGATCCCGGCGCTGGATAAAGAACTGAAAGCGAAAGGTAAGAGCGCGCTGATGTTCAACCTGCAAGAACCGTACTTCACCTGGCCGCTGATTGCTGCTGACGGGGGTTATGCGTTCAAGTATGAAAACGGCAAGTACGACATTAAAGACGTGGGCGTGGATAACGCTGGCGCGAAAGCGGGTCTGACCTTCCTGGTTGACCTGATTAAAAACAAACACATGAATGCAGACACCGATTACTCCATCGCAGAAGCTGCCTTTAATAAAGGCGAAACAGCGATGACCATCAACGGCCCGTGGGCATGGTCCAACATCGACACCAGCAAAGTGAATTATGGTGTAACGGTACTGCCGACCTTCAAGGGTCAACCATCCAAACCGTTCGTTGGCGTGCTGAGCGCAGGTATTAACGCCGCCAGTCCGAACAAAGAGCTGGCAAAAGAGTTCCTCGAAAACTATCTGCTGACTGATGAAGGTCTGGAAGCGGTTAATAAAGACAAACCGCTGGGTGCCGTAGCGCTGAAGTCTTACGAGGAAGAGTTGGCGAAAGATCCACGTATTGCCGCCACTATGGAAAACGCCCAGAAAGGTGAAATCATGCCGAACATCCCGCAGATGTCCGCTTTCTGGTATGCCGTGCGTACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGTCGATGAAGCCCTGAAAGACGCGCAGACTAATTCGAGCTCGAACAACAACAACAATAACAATAACAACAACCTCGGGATCGACGAAAACCTGTACCTCACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTTCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATGGCGCGCCTGTAGGACGTCGACGGTACCATCGATACGCGTTCGAAGCTTAATTAGCTGAGCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAAGCTAGCTTGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATTTTTTTAAGGCAGTTATTGGTGCCCTTAAACGCCTGGGGTAATGACTCTCTAGCTTGAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCCTCTAGATTACGTGCAGTCGATGATAAGCTGTCAAACATGAGAATTGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCGGAATTTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCTAGAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAC

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColE1-type bacterial origin of replication

6471

5789

negative selection marker

ccdB

ccdB death cassette gene (lost in with-insert form)

2643

2852

promoter

T5 promoter

recombination site

AttR1

AttR1 recombination site

1316

1440

recombination site

AttR2

AttR2 recombination site

3020

2896

repressor protein gene

LacI

LacI coding region

4132

5211

selectable marker

CmR

chloramphenicol resistance gene (CmR) (Lost in with-insert form)

1549

2205

selectable marker

AmpR

ampicillin resistance gene

7228

6569

tag

MBP

N-terminal MBP

148

1246

tag

His

N-terminal 8xHis tag

128

145

Gene

Gene Symbol :

RNPC3

Symbol Nomenclature :

RNPC3

SYNONYM :

RBM40; RNP; SNRNP65

Designation :

Full Nomenclature :

RNA-binding region (RNP1, RRM) containing 3

GENEID :

55599

HGNC :

18666

Vega :

OTTHUMG00000166613

Target GenBank:

BC010697

Reference Sequence Alignment

  

HsCD00084367      ------------------------------------------------------------
NM_017619.3       ATGGCAGCTCCCGAGCAGCCGCTTGCGATATCAAGGGGATGCACGAGCTCCTCCTCGCTT
                                                                              

HsCD00084367      ------------------------------------------------------------
NM_017619.3       TCCCCGCCTCGGGGCGACCGAACCCTTCTGGTCAGGCACCTGCCGGCTGAGCTTACTGCT
                                                                              

HsCD00084367      ------------------------------------------------------------
NM_017619.3       GAGGAGAAAGAGGACTTGCTGAAGTACTTCGGGGCTCAGTCTGTGCGGGTCCTGTCAGAT
                                                                              

HsCD00084367      ------------------------------------------------------------
NM_017619.3       AAGGGGCGACTGAAACATACAGCTTTTGCCACATTCCCTAATGAAAAAGCAGCTATAAAG
                                                                              

HsCD00084367      ------------------------------------------------------------
NM_017619.3       GCATTGACAAGACTCCATCAACTGAAACTTTTAGGTCATACTTTAGTCGTTGAATTTGCA
                                                                              

HsCD00084367      ------------------------------------------------------------
NM_017619.3       AAAGAGCAAGATCGAGTTCACTCCCCATGTCCCACTTCAGGCTCTGAAAAAAAAAAAAGG
                                                                              

HsCD00084367      ------------------------------------------------------------
NM_017619.3       TCTGATGACCCTGTCGAAGATGATAAAGAAAAAAAAGAACTTGGTTATTTAACAGTAGAA
                                                                              

HsCD00084367      ------------------------------------------------------------
NM_017619.3       AATGGAATTGCACCAAACCATGGGCTGACTTTTCCTTTAAATTCATGCCTCAAGTATATG
                                                                              

HsCD00084367      ------------------------------------------------------------
NM_017619.3       TACCCACCACCTTCCAGCACAATCCTAGCAAACATTGTAAATGCCTTGGCAAGCGTGCCT
                                                                              

HsCD00084367      ------------------------------------------------------------
NM_017619.3       AAGTTCTATGTACAGGTCCTTCATCTTATGAATAAAATGAATTTGCCCACACCTTTTGGA
                                                                              

HsCD00084367      ------------------------------------------------------------
NM_017619.3       CCAATTACTGCGCGACCTCCCATGTATGAAGACTATATGCCATTGCATGCACCTCTTCCA
                                                                              

HsCD00084367      ------------------------------------------------------------
NM_017619.3       CCCACATCTCCTCAGCCACCTGAGGAACCTCCTTTGCCAGACGAGGATGAGGAATTATCT
                                                                              

HsCD00084367      ---ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCAACAAATTA
NM_017619.3       AGTGAAGAATCAGAATATGAAAGCACTGATGATGAGGACCGACAGAGAATGAACAAATTA
                     ..*..:* . *.:*:.**. ..   . **:.*  :  .. : ...:  *********

HsCD00084367      ATGGAACTAGCAAATCTTCAGCCCAAAAGACCTAAAACAATAAAGCAGCGCCATGTGAGA
NM_017619.3       ATGGAACTAGCAAATCTTCAGCCCAAAAGACCTAAAACAATAAAGCAGCGCCATGTGAGA
                  ************************************************************

HsCD00084367      AAAAAGAGAAAAATAAAGGATATGTTGAATACACCTTTGTGTCCTTCACACAGCAGTTTA
NM_017619.3       AAAAAGAGAAAAATAAAGGATATGTTGAATACACCTTTGTGTCCTTCACACAGCAGTTTA
                  ************************************************************

HsCD00084367      CATCCAGTGCTGTTACCTTCAGATGTATTTGACCAACCACAACCTGTAGGTAACAAAAGA
NM_017619.3       CATCCAGTGCTGTTACCTTCAGATGTATTTGACCAACCACAACCTGTAGGTAACAAAAGA
                  ************************************************************

HsCD00084367      ATTGAATTCCATATATCTACCGACATGCCAGCTGCATTTAAGAAAGATTTAGAAAAGGAA
NM_017619.3       ATTGAATTCCATATATCTACCGACATGCCAGCTGCATTTAAGAAAGATTTAGAAAAGGAA
                  ************************************************************

HsCD00084367      CAAAATTGTGAGGAAAAAAATCATGATTTACCTGCTACTGAAGTTGATGCATCCAATATA
NM_017619.3       CAAAATTGTGAGGAAAAAAATCATGATTTACCTGCTACTGAAGTTGATGCATCCAATATA
                  ************************************************************

HsCD00084367      GGATTTGGAAAAATCTTCCCCAAACCTAATTTGGACATCACAGAGGAGATTAAAGAAGAC
NM_017619.3       GGATTTGGAAAAATCTTCCCCAAACCTAATTTGGACATCACAGAGGAGATTAAAGAAGAC
                  ************************************************************

HsCD00084367      TCTGATGAAATGCCTTCAGAATGTATTTCTAGAAGGGAATTGGAAAAGGGCAGAATTTCT
NM_017619.3       TCTGATGAAATGCCTTCAGAATGTATTTCTAGAAGGGAATTGGAAAAGGGCAGAATTTCT
                  ************************************************************

HsCD00084367      AGAGAAGAAATGGAAACACTTTCAGTTTTCAGAAGTTATGAACCGGGTGAACCAAACTGT
NM_017619.3       AGAGAAGAAATGGAAACACTTTCAGTTTTCAGAAGTTATGAACCGGGTGAACCAAACTGT
                  ************************************************************

HsCD00084367      AGAATTTATGTAAAGAATTTAGCTAAACATGTTCAAGAAAAGGACCTTAAATATATTTTT
NM_017619.3       AGAATTTATGTAAAGAATTTAGCTAAACATGTTCAAGAAAAGGACCTTAAATATATTTTT
                  ************************************************************

HsCD00084367      GGAAGATATGTTGACTTTTCATCAGAAACACAGCGGATCATGTTTGATATACGTTTGATG
NM_017619.3       GGAAGATATGTTGACTTTTCATCAGAAACACAGCGGATCATGTTTGATATACGTTTGATG
                  ************************************************************

HsCD00084367      AAAGAAGGTCGTATGAAAGGACAAGCTTTCATTGGACTTCCTAATGAAAAAGCAGCAGCA
NM_017619.3       AAAGAAGGTCGTATGAAAGGACAAGCTTTCATTGGACTTCCTAATGAAAAAGCAGCAGCA
                  ************************************************************

HsCD00084367      AAAGCCTTAAAGGAAGCTAATGGATATGTGCTTTTTGGAAAACCCATGGTGGTTCAGTTT
NM_017619.3       AAAGCCTTAAAGGAAGCTAATGGATATGTGCTTTTTGGAAAACCCATGGTGGTTCAGTTT
                  ************************************************************

HsCD00084367      GCTCGATCTGCTAGACCAAAACAAGATCCTAAGGAAGGAAAAAGAAAGTGTTAATAGGAC
NM_017619.3       GCTCGATCTGCTAGACCAAAACAAGATCCTAAGGAAGGAAAAAGAAAGTGTTAA------
                  ******************************************************      

HsCD00084367      CCAGCTTTCTTGTACAAAGTGGT
NM_017619.3       -----------------------