9130427A09Rik (Mus musculus) in pVP16

Explanation of Terms

Gene:
Gene Symbol:  Gramd3
Gene Name:  GRAM domain containing 3
Original Clone ID:
1034.1.03_GO.34651
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pVP16
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Center for Eukaryotic Structural Genomics
MmCD00084388
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1359nts
Open reading frame: 28 to 1386

ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCGTGAAGAAACGG
CTTTCCTCCAGTGACAATGTGTTCAAGTTCGAGATCCCCAGCAACTCGAAGACCAGTGCT
GAGGCCCCACACAGCTCCACTGACAGCCCAAGCTCCGTGTTTCTCAGCTCAGAGGCTGAG
AATGGTGCGGAGGACAGAAGGAGGGTCAGCAAGTCGCCAACAGCCCAATCTCCTACCTCA
TCTGTGGAGGCCGAGTCCCCGGACCAGAAGAGGAGCCTTGGCCTGTGGTCAAAATCCAGT
TTTGATGGTTCCAGTTTGTTGAGTGATAAGAATGACTGTAAAACTGAAAGCAAGACTGAC
TCTAAGACCGAAAGAAAAAAGTCTTCATCGTCTAGTCAGTACAAGGCGAACATGCACTTT
CACAAGTTATTCCTGGATGTCCCAACTGAAGAGCCACTGAGGCAAAGCTTTACCTGTGCT
CTTCAGAAAGAAATTCTGTACCAAGGAAAGCTCTTTGTGTCGGAAAACTGGATTTGTTTT
CATTCCAAGGTCTTTGGAAAAGACACTAAGATCTCCATCCCAGCTTTCTCGGTAACCTTG
ATCAAGAAAACCAAAACTGCCCTTCTGGTGCCAAACGCCCTGATCATCGCGACGGTCACA
GACAGGTACATATTCGTTTCCTTGCTGTCCAGAGACTCAACCTACAAACTGATAAAATCT
GTGTGCGGACACTTAGAGAAAACAAGTGTTGGTAACAGTCCCAACCCATCTTCTGCTGAA
AACAGTTTCCGGGCAGATCGCCCTTCGTCTCTGCCTCTGGATTTCAATGATGAATTCTCA
GACCTGGACGGAGTGGTTCGACAACGAAGGCAAGACTTGGAAGGATACAGCAGCTCAGGA
TCCCAGACTCCGGAATCTGAGAACTCCCGAGACTTCCACGTGACCGAATCTCAGACAGTT
CTGAATGTCACAAAGGGAGAAACAAAACCACCTCGGACAGATGCCCACGGGAGCAGAGCG
CCTGATGGAAAAGCCAAGATCCTGCCTGCCCATGGTCAATCAGAAACTATTGGAATTTTA
CATAAAATGGAGTCTCGGAAGTGCCCGACTCTCCATCACATCCTGATAGTCTATGCAATT
ATTGTCTGTGCCCTGATCATCTCCACCTTCTACATGAGATACAGGATTAACACTCTGGAG
GAGCGCCTGGGGACGCTGACGTCCATCATGGATCCCCACAGTACTGAGCAGACAGCCCCA
TCCGGCCTGGGATCACAAATGCAGTTAAATGTGGAGGTCCTCTGCCAAGAACTTACAGCT
AACATAGTGAAATTGGAAAAGATACAAAACAACTTACAAAAGCTGCTTGAGAATGGTGAC
TGATAGGACCCAGCTTTCTTGTACAAAGTGGT

Protein sequence predicted based on nucleotide sequence

ENLYFQSVKKRLSSSDNVFKFEIPSNSKTSAEAPHSSTDSPSSVFLSSEAENGAEDRRRV
SKSPTAQSPTSSVEAESPDQKRSLGLWSKSSFDGSSLLSDKNDCKTESKTDSKTERKKSS
SSSQYKANMHFHKLFLDVPTEEPLRQSFTCALQKEILYQGKLFVSENWICFHSKVFGKDT
KISIPAFSVTLIKKTKTALLVPNALIIATVTDRYIFVSLLSRDSTYKLIKSVCGHLEKTS
VGNSPNPSSAENSFRADRPSSLPLDFNDEFSDLDGVVRQRRQDLEGYSSSGSQTPESENS
RDFHVTESQTVLNVTKGETKPPRTDAHGSRAPDGKAKILPAHGQSETIGILHKMESRKCP
TLHHILIVYAIIVCALIISTFYMRYRINTLEERLGTLTSIMDPHSTEQTAPSGLGSQMQL
NVEVLCQELTANIVKLEKIQNNLQKLLENGD**

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1359
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in GO.34651
Recommended expression in:
Not Applicable