Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
1064.1.37_GO.35062
Keyword:
None
Species:
Oryza sativa
Type:
cDNA
Vector Name:
pVP16
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
PSI
Center for Eukaryotic Structural Genomics
OsCD00084409
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1269nts
Open reading frame: 51 to 1319

GGATCGACGAAAACCTGTACCTCACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCGCGGAGCACGACCTGACGGCGCGGATGGCGGGGCACCTGGACTGCCACC
TGGTGCTCCCGCTGCTGGAGTTCCTGCAGGAGCGGCACCTCTACCCGGAGGAGGAGATCC
TCGAGGCGAAGATCCGCCTCCTCCGGGGCACCAACATGGTCGACTACGCCATGGACATCC
ACAAGTCGCGCTACGGCACCGACGACGTCCCCGAGGACATGGTCAAGCGCCGCGCCGAGG
TCGTCTCCAGGCTCACCTCGCTCGGCGAGGCCATCGACAACATCCACCAGAACCAGCAGA
TTGGTCCTGATCAGATTGAGACTCTGTATCAGTATGCCAAATTTCAGTTTGACTGTGGGA
ATTACTCATTTGCTGCTCAGTACTTGCATCAATACCGTGCTCTGTGCACAAACATTGAGA
GGAGCTTGAGTGCCCTGTGGGGAAAGCTGGCAGCTGAGATTTTGATGCAGAACTGGGATG
TTGCACTGGAGGAGCTCAACCGTTTGAAGGAGATTATTGATTCAAAGAACTTCTCTTCAC
CGCTAAATCAGCTTCAGAATAGGATATGGCTGATGCACTGGAGTATCTTTATCTTCTACA
ACCACGAAAACGGCAGAAATGGGATCATAGATCTGTTCTTCCAGGAGAGGTACTTGAATG
CAATCCAGACAAATGCACCACATCTTCTGAGATACCTTGCTACAGCAGTTGTTGTGAACA
AAAGGAGAAGGAATATGCTTAAGGAGTTGATTAAAGTCATTCAGCAGGAGCAGCACAGCT
ACAAGGATCCCATTACTGAATTTCTGGAATGCTTGTTTGTTAACTATGATTTTGATGGCG
CACAACAGAAACTCATAGAGTGTGAGGAGGTCATATTGAATGATCCTTTCCTGGGAAAGC
GCATTGAGGAAGGGAATTCCATCACTGTTCCTTTGAGAGATGAATTCCTTGAAAATGCTC
GTCTGTTTATCTTTGAAACTTACTGCCGTATTCATCGGTCCATTGATATTGGCATGCTCT
CTCAGAAACTGAACATGCGCTATGATGAAGGGGAGTTGTGGATAATGAACTTAGTTAGAA
ATTCAAAGCTCGATGCCAAGATTGATTCAGTGTCTGGAACTCTTATCATGACGACCAATC
ATATTAACATCCACGAGCAGTTTATCGAAAGCTTGAAGAATCTTAACATGCGGACTTCCA
TGCTAGCAAAGAACATTGTGGAGCCAGCTCAAGCAATGCAACAGGCAACTCGGTGATAGG
ACCCAGCTTTCTTGTACAAAGTGGTGATGGCGCGCCTGTAGGACGTCGA

Protein sequence predicted based on nucleotide sequence

ENLYFQSAEHDLTARMAGHLDCHLVLPLLEFLQERHLYPEEEILEAKIRLLRGTNMVDYA
MDIHKSRYGTDDVPEDMVKRRAEVVSRLTSLGEAIDNIHQNQQIGPDQIETLYQYAKFQF
DCGNYSFAAQYLHQYRALCTNIERSLSALWGKLAAEILMQNWDVALEELNRLKEIIDSKN
FSSPLNQLQNRIWLMHWSIFIFYNHENGRNGIIDLFFQERYLNAIQTNAPHLLRYLATAV
VVNKRRRNMLKELIKVIQQEQHSYKDPITEFLECLFVNYDFDGAQQKLIECEEVILNDPF
LGKRIEEGNSITVPLRDEFLENARLFIFETYCRIHRSIDIGMLSQKLNMRYDEGELWIMN
LVRNSKLDAKIDSVSGTLIMTTNHINIHEQFIESLKNLNMRTSMLAKNIVEPAQAMQQAT
R**

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1269
Mutations:
Information not provided

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in GO.35062
Recommended expression in:
Not Applicable