ATP6V1C1 (Homo sapiens) in pVP16

Explanation of Terms

Gene:
Gene Symbol:  ATP6V1C1
Gene Name:  ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1
Original Clone ID:
1098.1.55_GO.35447
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pVP16
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Center for Eukaryotic Structural Genomics
HsCD00084478
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1170nts
Open reading frame: 28 to 1197

ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCACTGAGTTCTGG
CTTATATCTGCTCCTGGGGAGAAAACCTGTCAGCAAACATGGGAGAAATTGCATGCGGCA
ACTTCAAAGAACAATAATCTTGCTGTCACTTCCAAGTTCAATATTCCTGACTTAAAGGTT
GGCACGTTGGATGTCTTGGTTGGCTTGTCAGATGAACTGGCTAAACTGGATGCATTTGTA
GAAGGAGTGGTTAAGAAAGTAGCTCAATACATGGCTGATGTATTGGAAGATAGCAAAGAC
AAAGTTCAAGAGAATCTGTTGGCTAATGGAGTGGACTTGGTTACTTATATAACAAGGTTC
CAGTGGGACATGGCCAAATATCCAATCAAGCAGTCCCTGAAAAATATTTCTGAAATAATT
GCCAAGGGAGTAACTCAGATTGATAATGACCTGAAATCTCGAGCATCTGCATACAATAAC
CTGAAAGGAAATCTTCAGAATTTGGAACGAAAGAATGCAGGAAGTTTGCTAACTAGAAGT
CTAGCAGAAATTGTGAAGAAGGATGACTTTGTTCTTGATTCAGAGTATCTCGTCACATTA
CTGGTAGTAGTTCCCAAGTTAAACCACAACGACTGGATTAAGCAGTATGAAACACTAGCC
GAAATGGTAGTTCCAAGGTCTAGCAATGTTCTTTCAGAGGACCAAGACAGTTACCTGTGT
AATGTCACCTTGTTTAGGAAGGCAGTTGATGACTTCAGACACAAAGCCAGAGAAAACAAA
TTCATTGTTCGTGACTTCCAGTATAATGAAGAGGAGATGAAAGCAGATAAAGAAGAAATG
AACAGGCTTTCTACTGATAAGAAAAAACAATTTGGACCACTTGTACGGTGGCTGAAAGTG
AATTTTAGTGAAGCATTTATTGCATGGATTCACGTGAAAGCATTACGGGTTTTCGTTGAG
TCTGTTTTAAGGTATGGCTTGCCAGTGAACTTCCAAGCAATGCTACTTCAGCCCAATAAG
AAAACTTTGAAGAAACTGAGAGAAGTATTACATGAATTGTATAAACATCTAGACAGCAGT
GCAGCAGCTATTATTGATGCTCCTATGGATATTCCAGGTTTAAACCTGAGTCAACAAGAA
TACTACCCCTATGTGTACTACAAGATTGATTGCAACTTGCTGGAATTCAAGTGATAGGAC
CCAGCTTTCTTGTACAAAGT

Protein sequence predicted based on nucleotide sequence

ENLYFQSTEFWLISAPGEKTCQQTWEKLHAATSKNNNLAVTSKFNIPDLKVGTLDVLVGL
SDELAKLDAFVEGVVKKVAQYMADVLEDSKDKVQENLLANGVDLVTYITRFQWDMAKYPI
KQSLKNISEIIAKGVTQIDNDLKSRASAYNNLKGNLQNLERKNAGSLLTRSLAEIVKKDD
FVLDSEYLVTLLVVVPKLNHNDWIKQYETLAEMVVPRSSNVLSEDQDSYLCNVTLFRKAV
DDFRHKARENKFIVRDFQYNEEEMKADKEEMNRLSTDKKKQFGPLVRWLKVNFSEAFIAW
IHVKALRVFVESVLRYGLPVNFQAMLLQPNKKTLKKLREVLHELYKHLDSSAAAIIDAPM
DIPGLNLSQQEYYPYVYYKIDCNLLEFK**

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1170
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
Find experimental protocols in GO.35447
Recommended expression in:
Not Applicable