DNASU Plasmid | TM0895 (T. maritima ) in pMH2T7 (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: TM0895

Gene Name: glycogen synthase

Original Clone ID:

7497

Keyword:

None

Species:

Thermotoga maritima

Type:

cDNA

Vector Name:

pMH2T7

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Joint Center for Structural Genomics

TmCD00084673

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1461nts

Open reading frame: 51 to 1511

AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGAAAGTGG
TATTCGTTTCTTACGAGGTTTTTCCGTTCGCGAAAGTAGGAGGATTGGCGGACGTTGCTG
GAACTCTTCCAAAGTACTTGAAGAAACACGGGGTGGATGTCACGATAGTTATGCCGAAAC
ACAGAATCGTTGAAAAAAATGCGGAGAAATTTGGGTATGAGATCAAGAAAGTAGCCGAAA
GTCTCTCTGTTTCTCATGTCAAAACCGATCAAAAGTTCGATATATATGAATCCGTTCTTC
CCGGTAGCGATGTGAAAACGTATTTCGTTGCCAACGATTACTATTTCTCTGCGGAAGATG
TCTACGCGGGGCCAGATCTTGGTGAACAGGCGATTTTCTTCTGTGCGGCCACTCTGGATC
TGGTAAAACATCTTGATTTGAAGCCCGATATCGTCCATGTGAACGACTGGCAAACCGCTT
TGATCCCCGTCTATTTGAAAACTGTGTACAGGGACGATCCTTACTTTTCCAGAACGGCCA
CGGTTTTGACAATACACAACCTTGGATACCAGGGCGTGTTCGATCCAAAGTACCTCTCTT
TTGCAGGCCTTCCCGATTATGTTTACACTATCGACGGTCTTGAGTTCTACGGACAGCTGA
ATTTCCTCAAGGGCGGAATAGTGTTCAGCGATGTGATAAACACGGTTAGCCCCACTTACG
CCGAGGAAATTCAGACGGAAGAGTATGGTGAGAAACTCGATGGTGTACTGAGAATGCGCT
CGAAGGATCTCTACGGTATATTGAACGGAATAGATTACGAGCTTTATAACCCAGCCACGG
ACAGATACATTTACGTCAACTACGACGTGAATCGTCTTGAACTCAAGTGGGAAAACAAGG
TGAAGCTCCAGGAAGAACTGGGACTTCCCGTTAACAAGGAAACGGCTGTAGCCGGGTTGA
TCAGCAGGCTCGTCCCTCAAAAAGGTCTGGACCTTCTGGTTGATGTGATGGATTATCTGA
CGCTCTTTGATCTTCAGATCGTTGTTCTAGGAACAGGAGATGAACAGTACGAGAACGCTT
TCAGAAAGTTCCAGGAAAGGTATCCAGACAAAGTATCAGCGAACATAAAGTTCGATGTCG
AGCTGGCGCAGAAGATATACGCCGGTGCGGACATATTCCTCATGCCGAGCAGATACGAAC
CGTGCGGTTTGGGACAGATGTTCAGCATGAGATACGGAACAATCCCCGTTGTGAGATACA
CGGGAGGCCTGGCGGATACTGTGAAAGAATACGATCCACAATCGATGGAAGGTACGGGTT
TTGGATTCAAGAAGTACGATTCCGCTCATCTTTTGAAGGCTGTTTCGAAGGCTTTGCACT
TTTACTACAGGGAAAAAGATCATTGGAGGAGAATCATGACAAATGCCATGAACACGGATC
TGTCCTGGGACAGGTCCGCCAAAGAGTACGTTGATCTTTACAAAAAAGCCCTTGCAAAGG
TTGGAAGGTAATTAATTAACTTTAAGAAGGAGATATACATACCCGGGTAAGTTTAAACGG
T

Protein sequence predicted based on nucleotide sequence

MKVVFVSYEVFPFAKVGGLADVAGTLPKYLKKHGVDVTIVMPKHRIVEKNAEKFGYEIKK
VAESLSVSHVKTDQKFDIYESVLPGSDVKTYFVANDYYFSAEDVYAGPDLGEQAIFFCAA
TLDLVKHLDLKPDIVHVNDWQTALIPVYLKTVYRDDPYFSRTATVLTIHNLGYQGVFDPK
YLSFAGLPDYVYTIDGLEFYGQLNFLKGGIVFSDVINTVSPTYAEEIQTEEYGEKLDGVL
RMRSKDLYGILNGIDYELYNPATDRYIYVNYDVNRLELKWENKVKLQEELGLPVNKETAV
AGLISRLVPQKGLDLLVDVMDYLTLFDLQIVVLGTGDEQYENAFRKFQERYPDKVSANIK
FDVELAQKIYAGADIFLMPSRYEPCGLGQMFSMRYGTIPVVRYTGGLADTVKEYDPQSME
GTGFGFKKYDSAHLLKAVSKALHFYYREKDHWRRIMTNAMNTDLSWDRSAKEYVDLYKKA
LAKVGR*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 1461

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DL41

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Find experimental protocols in 282764

Recommended expression in:

Not Applicable

Vector Name:

pMH2T7

Synonyms:

pMH-2-T7

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.

Comments:

None

Size (bp):

4084

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGTGTAATTAATTAACTTTAAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColEI origin of replication

1921

2603

promoter

T7 promoter

290

310

promoter

araC

araC promoter

256

285

selectable marker

AmpR

ampicillin resistance gene

1164

1823

tag

His

N-terminal 6x His tag

367

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

Gene

Gene Symbol :

TM0895

GENEID :

898569

Locus Tag :

TM0895

Target GenBank:

NP_228703

Protein

Structural Annotation Wiki :

TOPSAN

Swissprot ID :

Q9WZZ7

Clone

Cloning Information :

275694

HIP Master Clone ID :

205180

Original Clone ID :

7497

Experimental

TargetTrack - Experimental data :

282764

Organism

Species Specific ID:

898569