DNASU Plasmid | TM0760 (T. maritima ) in pMH2T7 (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: TM0760

Gene Name: lipopolysaccharide biosynthesis protein

Original Clone ID:

7422

Keyword:

None

Species:

Thermotoga maritima

Type:

cDNA

Vector Name:

pMH2T7

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

Discrepancy in reported linker sequence. Insert Sequence reports the actual sequence of the linker.

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Joint Center for Structural Genomics

TmCD00084692

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1197nts

Open reading frame: 51 to 1247

AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACTTGAACATAG
CCATACTCAATCACTACGCGAGCATACCAGAAGTGGGAAGCGCAGAAACGAGGCATTTCG
AACTTGCGAAGCGATTTGTAAGAGAAGGTCACAGAGTGGACATCTACATCGGGGATTTTT
CGCACCTCACTGGAAAAAGATGGAGCGAAACGTTCGGATGGAGCTTTTCTAAGGACGGTG
TGGATTTCATCGTCGTTGAGACAAGAGAGTACACCGGAAACTCCCTTTCGAGGCTTCTCT
CATCGATAGATTATTACAGAAACGGAAGGAAGAAGATCATTCAGAAGAGATACGACGTCA
TCATAGCGTCTTCTCCGCATCCGTTTTCCTGGAGCCTTGGCTGGTACTATGTGAGAAGAA
AAGGCGGAAAATTTTTCATCGAGATCAGGGATGTCTGGCCGGACGATCTTGTAAAACTCG
GTTCTCTGAGTTACCGTCATCCAGTTTCAAAGCTCTTCGACCACATGTGCAAAAAGTATT
ATCCGAAGGCGGATGGATTCATTTCTCTTGTTCCTGATCTTTCCGAGCACTTCAACAGAT
TGAAGGTTCGACCAGAAAATTTCATCTACATTCCCAACGGTGTTGATCTGAAGGTTTTCA
AAGATCCAGAACCGTGCCCGGTGGTGGATGAAGTCTTCTCAAAGGTGCCTGATGGCAAGA
TAAAGGTTGTCTACGCGGGATCCATCGTCGAACATAAGGGAATAAAAGAGTTCATAGAAA
CACTCTCAAAAGTGAACAAAAACTTGAGAGATCGTTTTGTGTTCTTTTTTGTCGGACCGT
CTCATGCGGATTATCTTGTTTCTGTGAAAGAAACGGCGAAAGATCTGCAGAACGTGTTCT
TTTTTGATCCTGTTCCGAAAAGGTGTGTTCCCTACCTTCTTCAGAAGGCAGATGTTCTCC
TCTTCACTCTCTCTCAAACCGTGATGGATCACCCCGCCGTGAGTTCCTACAAGGTGGTAG
ACTACATGGCCTCTGGAAAACCAGTTCTCTGTGTCGATATCGAAAATCTCCCTTTCAAGA
AAACAAAGGGTGCTGTCTTTTTCAGGGAGGATAATCTCGAAGAGGCGCTGAGGAGGATCT
TCGAGGAGGATCTTTCAGAACTCGGCAGAAGAAACAGAGAGTATGTGGAGAGAGAAAGAG
ACTGGGATCGACTTTACGAAAAGTTAAGAAGTTTCGTACTCTCCTAAGTTTAAACGGTCT
CCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTC

Protein sequence predicted based on nucleotide sequence

LNIAILNHYASIPEVGSAETRHFELAKRFVREGHRVDIYIGDFSHLTGKRWSETFGWSFS
KDGVDFIVVETREYTGNSLSRLLSSIDYYRNGRKKIIQKRYDVIIASSPHPFSWSLGWYY
VRRKGGKFFIEIRDVWPDDLVKLGSLSYRHPVSKLFDHMCKKYYPKADGFISLVPDLSEH
FNRLKVRPENFIYIPNGVDLKVFKDPEPCPVVDEVFSKVPDGKIKVVYAGSIVEHKGIKE
FIETLSKVNKNLRDRFVFFFVGPSHADYLVSVKETAKDLQNVFFFDPVPKRCVPYLLQKA
DVLLFTLSQTVMDHPAVSSYKVVDYMASGKPVLCVDIENLPFKKTKGAVFFREDNLEEAL
RRIFEEDLSELGRRNREYVERERDWDRLYEKLRSFVLS*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 1197

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DL41

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Protein_Purified

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in 282630

Recommended expression in:

Not Applicable

Vector Name:

pMH2T7

Synonyms:

pMH-2-T7

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.

Comments:

None

Size (bp):

4084

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGTGTAATTAATTAACTTTAAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColEI origin of replication

1921

2603

promoter

T7 promoter

290

310

promoter

araC

araC promoter

256

285

selectable marker

AmpR

ampicillin resistance gene

1164

1823

tag

His

N-terminal 6x His tag

367

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

Gene

Gene Symbol :

TM0760

GENEID :

898428

Locus Tag :

TM0760

Target GenBank:

NP_228569

Protein

Structural Annotation Wiki :

TOPSAN

Swissprot ID :

Q9WZL9

Clone

Cloning Information :

275982

HIP Master Clone ID :

205468

Original Clone ID :

7422

Experimental

TargetTrack - Experimental data :

282630

Organism

Species Specific ID:

898428