DNASU Plasmid | TM1166 (T. maritima ) in pMH1 (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: TM1166

Gene Name: oxygen-independent coproporphyrinogen III oxidase

Original Clone ID:

7661

Keyword:

None

Species:

Thermotoga maritima

Type:

cDNA

Vector Name:

pMH1

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Joint Center for Structural Genomics

TmCD00084747

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1125nts

Open reading frame: 51 to 1175

AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGAAACTCG
CTGTTTACGTCCATGTTCCCTTTTGCAAGAGCAAGTGTGTGTACTGCGATTTTTACTCTG
TTGTCTCTGAAAAATTTGACGAGTATTTCTCTCATCTCCTGAGAGAAATCGATCTCTACG
AAGAAGTACTCAGCGAAAGCGAGATAAAAACCGTCTACTTCGGTGGAGGGACTCCTTCCG
TTGTCCCTCCTTCTTTTTTGAAAATGGTTTTGGAAAAACTGGAGAGAGTCTCCAGAGGTT
TCACACCAGATGAAATAACCATCGAGGTCAACCCGGAGTCTGTTGAAACAGAGAAACTGA
AAATCTACAAACAAATCGGAATCAACAGAATCAGTCTCGGTGTTCAGGCCTGCGATGACA
CTGTGCTGAAGAACGCTGGAAGGCTCTACAAAGAAGAAACCCTCAAGAAAAAAGCGAAGA
TAGTTCTGGAACAGTTCGAAAACGTGAATTTCGATTTTATTCTTGGACTTCCTGGTGAGA
CCGACATCACACTGAAGAAAGACTTCCGTTTCCTTGAAGAATTTCCCCCTCAGCACGTTT
CGCTATATCTCCTCGAGGTAGACGAAAGAACAAGACTTTTCGATCTCATTCAGAAAGGTC
TTGTAGAACTCCCCGAAGAAGACGATGTGGAAAGGAGGCACGACCTTTTCGTTGAATTTC
TGAAAGAACGAGGATTTCTCAGATACGAGATCTCGAACTTTGCCAAACCAGGGAAGAAAT
CTCTTCACAACCTCTTCTACTGGAGAAATGAAAACTATCTGGGACTCGGTGTTTCCGCAG
GTGGGCACATCGGAAGGTTTCGATACGTGAACGCCTCTGATCTTAAAGAGTACGAGGAAA
AAATCACCAAAGGAGAATTACCTTACGAATACGTGCACGAAAACACAGAAGAAGAGGAAG
CGCTGGAGACAGTCTTCATGGGTTTGAGGATAAAGGAAGGAGTAGAGTTAAACCGGGTGA
AAATTCTCCTTCCCCTGCTAGAAAAGTTACAGAAAAAGTACCCTTGTTATCTGAAGGTGA
AAAATGGTAAAATTTTTTTAAGCGAGGATGGTATGAATTTCTCAAAGAAGATACTCTCTG
ATCTCATCGAATGGTACAGGGAGGGCGGGAGATAAGTTTAAACGGTCTCCAGCTTGGCTG
TTTTGGCGGATGAGAGAAGATTTTC

Protein sequence predicted based on nucleotide sequence

MKLAVYVHVPFCKSKCVYCDFYSVVSEKFDEYFSHLLREIDLYEEVLSESEIKTVYFGGG
TPSVVPPSFLKMVLEKLERVSRGFTPDEITIEVNPESVETEKLKIYKQIGINRISLGVQA
CDDTVLKNAGRLYKEETLKKKAKIVLEQFENVNFDFILGLPGETDITLKKDFRFLEEFPP
QHVSLYLLEVDERTRLFDLIQKGLVELPEEDDVERRHDLFVEFLKERGFLRYEISNFAKP
GKKSLHNLFYWRNENYLGLGVSAGGHIGRFRYVNASDLKEYEEKITKGELPYEYVHENTE
EEEALETVFMGLRIKEGVELNRVKILLPLLEKLQKKYPCYLKVKNGKIFLSEDGMNFSKK
ILSDLIEWYREGGR*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 1125

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DL41

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Find experimental protocols in 283032

Recommended expression in:

Not Applicable

Vector Name:

pMH1

Synonyms:

pMH-1

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial (E. coli) expression vector, arabinose inducible expression, adds N-terminal 6xHis tag; amp resistance; restriction enzyme (Pml I/Psi I) or PIPE cloning.

Comments:

None

Size (bp):

4048

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTTTGGGCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGTGTTATAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColE1-type bacterial origin of replication

1887

2569

inducible trxn activator protein gene

araC

araC cds

3144

4019

promoter

pBAD

pBAD promoter

250

277

promoter

araC

araC promoter

256

285

selectable marker

AmpR

ampicillin resistance gene

1130

1789

tag

His

N-terminal 6xHis tag

364

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

trxn termination sequence

rrnB

rrnB T2 transcription terminator

794

820

Gene

Gene Symbol :

TM1166

GENEID :

898320

Locus Tag :

TM1166

Target GenBank:

NP_228972

Protein

SMART domain :

Elp3 : Elongator protein 3, MiaB family, Radical SAM

Structural Annotation Wiki :

TOPSAN

Swissprot ID :

Q9X0P9

Clone

Cloning Information :

276749

HIP Master Clone ID :

206235

Original Clone ID :

7661

Experimental

TargetTrack - Experimental data :

283032

Organism

Species Specific ID:

898320