Gene:
Gene Symbol:
TM1030
Gene Name:
TetR family transcriptional regulator
Original Clone ID:
7586
Keyword:
None
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH1
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 17444523
Title: Crystal structure of TM1030 from Thermotoga maritima at 2.3 A resolution reveals molecular details of its transcription repressor function.
Title: Crystal structure of TM1030 from Thermotoga maritima at 2.3 A resolution reveals molecular details of its transcription repressor function.
Authors:
PSI
Joint Center for Structural Genomics
Joint Center for Structural Genomics
TmCD00084786
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 603nts
Open reading frame: 51 to 653
AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGTGTTGTCGA
AGAGGGATGCAATACTGAAGGCAGCGGTTGAGGTTTTCGGGAAAAAAGGTTACGATCGAG
CCACAACAGACGAAATAGCAGAGAAAGCCGGGGTAGCCAAGGGTTTGATTTTTCACTATT
TCAAGAACAAAGAAGAACTGTACTATCAGGCATACATGTCCGTTACAGAAAAGCTCCAGA
AGGAGTTCGAGAATTTCCTTATGAAAAACAGGAATAGAGACATATTTGACTTCATGGAAC
GGTGGATAGAAAAGAAACTGGAGTACTCCGCTTCTCATCCCGAAGAAGCAGATTTTCTGA
TAACGCTCGTGAGCGTGGATGAAGGACTCAGGAAAAGAATACTTCTCGACCTCGAAAAAT
CACAGAGGGTCTTTTTTGACTTCGTAAGGGAAAAATTGAAAGATTTAGATCTCGCAGAAG
ATGTAACGGAGGAAATCGCTCTGAAATTTCTGATGTGGTTCTTTTCCGGATTTGAAGAAG
TCTACCTGAGGACCTATCAAGGTAAGCCCGAACTGCTGAAAAGAGACATGAACACACTCG
TTGAAGAAGTGAAGGTCATGCTCAGGATTTTGAAGAAAGGCATGACAAAATAAGTTTAAA
CGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTC
Protein sequence predicted based on nucleotide sequence
VLSKRDAILKAAVEVFGKKGYDRATTDEIAEKAGVAKGLIFHYFKNKEELYYQAYMSVTE KLQKEFENFLMKNRNRDIFDFMERWIEKKLEYSASHPEEADFLITLVSVDEGLRKRILLD LEKSQRVFFDFVREKLKDLDLAEDVTEEIALKFLMWFFSGFEEVYLRTYQGKPELLKRDM NTLVEEVKVMLRILKKGMTK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
603
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DL41
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 282897
Recommended expression in:
Not Applicable
Vector Name:
pMH1
Synonyms:
pMH-1
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial (E. coli) expression vector, arabinose inducible expression, adds N-terminal 6xHis tag; amp resistance; restriction enzyme (Pml I/Psi I) or PIPE cloning.
Comments:
None
Size (bp):
4048
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type bacterial origin of replication
1887
2569
inducible trxn activator protein gene
araC
araC cds
3144
4019
promoter
pBAD
pBAD promoter
250
277
promoter
araC
araC promoter
256
285
selectable marker
AmpR
ampicillin resistance gene
1130
1789
tag
His
N-terminal 6xHis tag
364
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
trxn termination sequence
rrnB
rrnB T2 transcription terminator
794
820
