DNASU Plasmid | gcvT (Thermotoga maritima) in pMH1 (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: gcvT

Gene Name: glycine cleavage system aminomethyltransferase T

Original Clone ID:

7094

Keyword:

None

Species:

Thermotoga maritima

Type:

cDNA

Vector Name:

pMH1

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Joint Center for Structural Genomics

TmCD00084863

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1095nts

Open reading frame: 51 to 1145

AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGAAGAGAA
CGCCTCTATTTGAAAAGCATGTTGAACTCGGTGCGAAAATGGTAGATTTCGCGGGGTGGG
AGATGCCTCTTTATTACACATCCATATTCGAAGAAGTGATGGCAGTCAGAAAATCGGTGG
GGATGTTCGATGTCTCTCACATGGGGGAATTTCTCGTTAAAGGGCCCGAGGCGGTTTCTT
TTATTGACTTTCTTATAACGAACGACTTTTCTTCACTTCCGGATGGTAAAGCGATTTACT
CTGTCATGTGCAATGAAAACGGTGGAATCATCGATGATCTTGTTGTGTACAAGGTGAGTC
CTGATGAAGCTCTCATGGTGGTGAACGCGGCAAACATTGAAAAGGACTTCAACTGGATAA
AATCCCATTCGAAGAATTTCGATGTCGAAGTATCGAACATTTCAGATACGACAGCGCTCA
TAGCGTTCCAGGGGCCAAAAGCACAGGAGACCCTCCAGGAGCTTGTTGAAGACGGTCTCG
AGGAAATTGCCTATTATTCTTTCAGAAAGAGTATTGTAGCCGGTGTAGAGACTCTTGTCT
CAAGAACGGGATACACGGGTGAGGACGGTTTCGAACTGATGCTTGAAGCGAAGAATGCAC
CGAAGGTCTGGGATGCTTTGATGAATCTGCTCAGAAAAATCGATGGCAGACCCGCAGGTC
TTGGGGCGAGAGACGTTTGCCGACTCGAGGCGACTTATTTGCTGTACGGTCAGGACATGG
ATGAGAACACGAATCCTTTCGAAGTGGGACTTTCCTGGGTTGTAAAACTGAACAAAGACT
TCGTGGGGAAGGAAGCGCTGCTGAAAGCGAAGGAAAAAGTGGAAAGAAAACTTGTGGCGC
TTGAGCTCTCTGGAAAAAGAATAGCAAGAAAAGGCTATGAAGTTTTAAAGAATGGAGAGA
GAGTGGGAGAGATCACAAGTGGTAACTTCTCTCCAACTCTTGGGAAGTCGATAGCGCTGG
CTCTCGTTTCAAAATCGGTGAAGATAGGAGACCAACTGGGAGTGGTGTTTCCTGGTGGAA
AGCTTGTAGAAGCTCTGGTAGTAAAAAAACCATTTTACAGAGGCAGTGTGAGGAGGGAGG
TTTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTC

Protein sequence predicted based on nucleotide sequence

MKRTPLFEKHVELGAKMVDFAGWEMPLYYTSIFEEVMAVRKSVGMFDVSHMGEFLVKGPE
AVSFIDFLITNDFSSLPDGKAIYSVMCNENGGIIDDLVVYKVSPDEALMVVNAANIEKDF
NWIKSHSKNFDVEVSNISDTTALIAFQGPKAQETLQELVEDGLEEIAYYSFRKSIVAGVE
TLVSRTGYTGEDGFELMLEAKNAPKVWDALMNLLRKIDGRPAGLGARDVCRLEATYLLYG
QDMDENTNPFEVGLSWVVKLNKDFVGKEALLKAKEKVERKLVALELSGKRIARKGYEVLK
NGERVGEITSGNFSPTLGKSIALALVSKSVKIGDQLGVVFPGGKLVEALVVKKPFYRGSV
RREV*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 1095

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DL41

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Find experimental protocols in 282091

Recommended expression in:

Not Applicable

Vector Name:

pMH1

Synonyms:

pMH-1

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial (E. coli) expression vector, arabinose inducible expression, adds N-terminal 6xHis tag; amp resistance; restriction enzyme (Pml I/Psi I) or PIPE cloning.

Comments:

None

Size (bp):

4048

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTTTGGGCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGTGTTATAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColE1-type bacterial origin of replication

1887

2569

inducible trxn activator protein gene

araC

araC cds

3144

4019

promoter

pBAD

pBAD promoter

250

277

promoter

araC

araC promoter

256

285

selectable marker

AmpR

ampicillin resistance gene

1130

1789

tag

His

N-terminal 6xHis tag

364

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

trxn termination sequence

rrnB

rrnB T2 transcription terminator

794

820

Gene

Gene Symbol :

gcvT

GENEID :

897085

Locus Tag :

TM0211

Target GenBank:

NP_228026

Protein

Structural Annotation Wiki :

TOPSAN

Swissprot ID :

Q9WY54

Clone

Cloning Information :

276364

HIP Master Clone ID :

205850

Original Clone ID :

7094

Experimental

TargetTrack - Experimental data :

282091

Organism

Species Specific ID:

897085