Gene:
Gene Symbol:
thyX
Gene Name:
FAD-dependent thymidylate synthase
Original Clone ID:
7252
Keyword:
None
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH1
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 12211025
Title: Crystal structure of thy1, a thymidylate synthase complementing protein from Thermotoga maritima at 2.25 A resolution.
Title: Crystal structure of thy1, a thymidylate synthase complementing protein from Thermotoga maritima at 2.25 A resolution.
Authors:
PSI
Joint Center for Structural Genomics
Joint Center for Structural Genomics
TmCD00084968
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 663nts
Open reading frame: 51 to 713
AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGAAGATCG
ATATCTTGGACAAAGGATTCGTTGAACTTGTGGATGTGATGGGAAACGACCTCTCCGCTG
TACGGGCTGCCCGCGTTTCTTTCGATATGGGTTTGAAAGACGAAGAAAGGGATAGACATC
TCATCGAATATCTCATGAAACACGGTCACGAGACACCTTTCGAACATATTGTCTTCACTT
TCCACGTGAAAGCCCCTATATTCGTGGCGAGGCAGTGGTTCAGACACAGGATCGCTTCTT
ACAACGAACTGAGCGGCAGATACTCGAAGCTCTCCTACGAATTCTACATTCCCTCTCCCG
AACGCCTGGAAGGGTACAAAACGACCATCCCTCCCGAACGGGTGACGGAGAAGATCTCAG
AAATAGTCGATAAAGCGTATCGAACGTATCTGGAGTTGATAGAAAGCGGTGTTCCTCGAG
AAGTGGCAAGGATAGTGCTCCCTTTGAATCTGTACACGAGGTTTTTCTGGACTGTGAACG
CAAGAAGTCTCATGAACTTTTTGAACCTGAGGGCAGATTCTCACGCTCAGTGGGAAATTC
AACAGTACGCTCTGGCAATCGCGAGAATTTTCAAAGAGAAGTGTCCCTGGACTTTTGAGG
CATTTCTTAAGTATGCTTATAAAGGAGATATCCTGAAGGAGGTACAGGTATAAGTTTAAA
CGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTC
Protein sequence predicted based on nucleotide sequence
MKIDILDKGFVELVDVMGNDLSAVRAARVSFDMGLKDEERDRHLIEYLMKHGHETPFEHI VFTFHVKAPIFVARQWFRHRIASYNELSGRYSKLSYEFYIPSPERLEGYKTTIPPERVTE KISEIVDKAYRTYLELIESGVPREVARIVLPLNLYTRFFWTVNARSLMNFLNLRADSHAQ WEIQQYALAIARIFKEKCPWTFEAFLKYAYKGDILKEVQV*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
663
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DL41
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 282322
Recommended expression in:
Not Applicable
Vector Name:
pMH1
Synonyms:
pMH-1
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial (E. coli) expression vector, arabinose inducible expression, adds N-terminal 6xHis tag; amp resistance; restriction enzyme (Pml I/Psi I) or PIPE cloning.
Comments:
None
Size (bp):
4048
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type bacterial origin of replication
1887
2569
inducible trxn activator protein gene
araC
araC cds
3144
4019
promoter
pBAD
pBAD promoter
250
277
promoter
araC
araC promoter
256
285
selectable marker
AmpR
ampicillin resistance gene
1130
1789
tag
His
N-terminal 6xHis tag
364
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
trxn termination sequence
rrnB
rrnB T2 transcription terminator
794
820
