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Detailed Vector Information: pHM6g
Explanation of Terms
Clone: EvNO00085135
Vector: pHM6g
Additional Annotations
Gene:
Gene Symbol:
No insert
Gene Name:
  No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pHM6g
Source:
Berkeley Structural Genomics Center
Description:
"Vector that adds N-terminal 6xHis tag, MBP, Tev protease site; kanamycin resistance; restriction enzyme cloning."
Comments:
Made unavailable due to incorrect and/or unavailable vector information
Publications:
None
Authors:
Berkeley Structural Genomics Center
PSI
Lawrence Berkeley National Laboratory
EvNO00085135
Price:
Login for Pricing
UNAVAILABLE
No restriction
Special MTA: PSI
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Vector Name:
pHM6g
Synonyms:
pET28a.HMV.6gly, pHM, pHMG, pHMGly
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Vector that adds N-terminal 6xHis tag, MBP, Tev protease site; kanamycin resistance; restriction enzyme cloning.
Comments:
None
Size (bp):
0
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Berkeley Structural Genomics Center
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
protease cleavage site
TEV
TEV protease cleavage site cds
0
0
tag
His
6xHis tag (after cleavage GHSGGGGGGH)
0
0
tag
MBP
maltose binding protein cds
0
0
Species Specific ID:
None
Target GenBank:
None