Gene:
Gene Symbol:
No insert
Gene Name:
No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pMK-Dest
Source:
None
Description:
with tag/fusion/marker assay, Gateway® clone, bacterial expression expression with CMV and CMV-F and attR1 and attR2 and lac promoter and pBR322 origin and NeoR and AttB2 and TEV; kanamycin resistance in bacterial, neomycin resistance in bacterial;
Comments:
None
Publications:
None
Authors:
Center for Personalized Diagnostics
Bianca
Mark Knappenberger
Krishna
Bianca
Mark Knappenberger
Krishna
EvNO00877066
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Host Type: bacterial Marker: neomycin
Host Type: bacterial Marker: neomycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Vector Name:
pMK-Dest
Synonyms:
''
Sequencing Primer:
Forward:
CMV forward
Reverse:
None
Description:
with tag/fusion/marker assay, Gateway® clone, bacterial expression expression with CMV and CMV-F and attR1 and attR2 and lac promoter and pBR322 origin and NeoR and AttB2 and TEV; kanamycin resistance in bacterial, neomycin resistance in bacterial;
Comments:
From Karen Anderson Lab, Produced by Bianca Varda, Krishna, and Mark Knappenberger
Size (bp):
3386
Parent Vector:
Name:
pMax-Cloning
Description:
Properties:
Gateway, bacterial expression, with tag/fusion/marker
Author Name:
Center for Personalized Diagnostics
Bianca
Mark Knappenberger
Krishna
Bianca
Mark Knappenberger
Krishna
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
CDS
TEV
TEV Cleavage site
1252
1272
Origin
pBR322 origin
2748
2130
primer
CMV-F
949
969
promoter
CMV
938
1019
promoter
lac promoter
1437
1460
recombination site
attR1
1288
1412
recombination site
attR2
1412
1312
recombination site
AttB2
2066
2090
selectable marker
NeoR
Neomycin/Kanamycin Resistance
2816
209
