PYC1 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH148285.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00008791
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3537nts
Open reading frame: 1 to 3537

ATGTCGCAAAGAAAATTCGCCGGCTTGAGAGATAACTTCAATCTCTTGGGTGAAAAGAAC
AAAATATTGGTGGCTAATAGAGGAGAAATTCCAATCAGAATTTTTCGTACCGCTCATGAA
CTGTCTATGCAGACGGTAGCTATATATTCTCATGAAGATCGTCTTTCAACGCACAAACAA
AAGGCTGACGAAGCATACGTCATAGGTGAAGTAGGCCAATATACCCCCGTCGGCGCTTAT
TTGGCCATTGACGAAATCATTTCCATTGCCCAAAAACACCAGGTAGATTTCATCCATCCA
GGTTATGGGTTCTTGTCTGAAAATTCGGAATTTGCCGACAAAGTAGTGAAGGCCGGTATC
ACTTGGATTGGCCCTCCAGCTGAAGTTATTGACTCCGTGGGTGATAAGGTCTCAGCTAGA
AACCTGGCAGCAAAAGCTAATGTGCCCACCGTTCCTGGTACACCAGGTCCTATAGAAACT
GTAGAGGAAGCACTTGACTTCGTCAATGAATACGGCTACCCGGTGATCATTAAGGCCGCC
TTTGGTGGTGGTGGTAGAGGTATGAGAGTCGTTAGAGAAGGTGACGACGTGGCAGATGCC
TTTCAACGTGCTACCTCCGAAGCCCGTACTGCCTTCGGTAATGGTACCTGCTTTGTGGAA
AGATTCTTGGACAAGCCAAAGCATATTGAAGTTCAATTGTTGGCCGATAACCACGGAAAC
GTGGTTCATCTTTTCGAAAGAGACTGTTCCGTGCAGAGAAGACACCAAAAGGTTGTCGAA
GTGGCCCCAGCAAAGACTTTACCCCGTGAAGTCCGTGACGCCATTTTGACAGATGCAGTT
AAATTGGCCAAAGAGTGTGGCTACAGAAATGCGGGTACTGCTGAATTCTTGGTTGATAAC
CAAAATAGACACTATTTCATTGAAATTAATCCAAGAATCCAAGTGGAACATACCATCACA
GAAGAAATTACCGGTATAGATATTGTGGCGGCTCAGATCCAAATTGCGGCAGGTGCCTCT
CTACCCCAGCTGGGCCTATTCCAGGACAAAATTACGACTCGTGGCTTTGCCATTCAGTGC
CGTATTACCACGGAAGACCCTGCTAAGAACTTCCAACCAGATACCGGTAGAATAGAAGTG
TACCGTTCTGCAGGTGGTAATGGTGTTAGACTGGATGGTGGTAACGCCTATGCAGGAACA
ATAATCTCACCTCATTACGACTCAATGCTGGTCAAATGCTCATGCTCCGGTTCCACCTAC
GAAATCGTTCGTAGAAAAATGATTCGTGCATTAATCGAGTTCAGAATTAGAGGTGTCAAG
ACCAACATTCCCTTCCTATTGACTCTTTTGACCAATCCAGTATTTATTGAGGGTACATAC
TGGACGACTTTTATTGACGACACCCCACAACTGTTCCAAATGGTTTCATCACAAAACAGA
GCCCAAAAACTTTTACATTACCTCGCCGACGTGGCAGTCAATGGTTCATCTATCAAGGGT
CAAATTGGCTTGCCAAAATTAAAATCAAATCCAAGTGTCCCCCATTTGCACGATGCTCAG
GGCAATGTCATCAACGTTACAAAGTCTGCACCACCATCCGGATGGAGGCAAGTGCTACTA
GAAAAGGGGCCAGCTGAATTTGCCAGACAAGTTAGACAGTTCAATGGTACTTTATTGATG
GACACCACCTGGAGAGACGCTCATCAATCTCTACTTGCAACAAGAGTCAGAACCCACGAT
TTGGCTACAATCGCTCCAACAACCGCACATGCCCTTGCAGGTGCTTTCGCCTTAGAATGT
TGGGGTGGTGCCACATTCGATGTTGCAATGAGATTTTTGCATGAGGATCCATGGGAACGT
TTGAGAAAATTAAGATCTCTGGTGCCTAATATTCCATTCCAAATGTTATTGCGTGGTGCC
AATGGTGTGGCTTATTCTTCATTGCCTGACAATGCTATTGACCATTTCGTCAAGCAAGCC
AAGGATAATGGTGTTGATATATTTAGAGTCTTTGATGCCTTAAATGACTTGGAACAATTG
AAGGTCGGTGTAGATGCTGTGAAGAAGGCAGGTGGTGTTGTAGAAGCCACTGTTTGTTTC
TCTGGGGATATGCTTCAGCCAGGCAAGAAATACAATTTGGATTACTACTTGGAAATTGCT
GAAAAAATTGTCCAAATGGGCACTCATATCCTGGGTATCAAAGATATGGCAGGTACCATG
AAGCCAGCAGCTGCCAAACTACTGATTGGATCTTTGAGGGCTAAGTACCCTGATCTCCCA
ATACATGTTCACACTCACGATTCTGCAGGTACTGCTGTTGCATCAATGACTGCGTGTGCT
CTGGCGGGCGCCGATGTCGTTGATGTTGCCATCAACTCAATGTCTGGTTTAACTTCACAA
CCATCAATCAATGCTCTGTTGGCTTCATTAGAAGGTAATATTGACACTGGTATTAACGTT
GAGCATGTCCGTGAACTAGATGCATATTGGGCAGAGATGAGATTGTTATACTCTTGTTTC
GAGGCTGACTTGAAGGGCCCAGATCCAGAAGTTTATCAACATGAAATCCCAGGTGGTCAA
TTGACAAACTTGTTGTTTCAAGCCCAACAATTGGGTCTTGGAGAACAATGGGCCGAAACA
AAAAGAGCTTACAGAGAAGCCAATTATTTATTGGGTGATATTGTCAAAGTTACCCCAACT
TCGAAGGTCGTTGGTGATCTGGCACAATTTATGGTCTCCAATAAATTAACTTCCGATGAT
GTGAGACGCCTGGCTAATTCTTTGGATTTCCCTGACTCTGTTATGGATTTCTTCGAAGGC
TTAATCGGCCAACCATATGGTGGGTTCCCAGAACCATTTAGATCAGACGTTTTAAGGAAC
AAGAGAAGAAAGTTGACTTGTCGTCCAGGCCTGGAACTAGAGCCATTTGATCTCGAAAAA
ATTAGAGAAGACTTGCAGAATAGATTTGGTGATGTTGATGAGTGCGACGTTGCTTCTTAT
AACATGTACCCAAGAGTTTATGAAGACTTCCAAAAGATGAGAGAAACGTATGGTGATTTA
TCTGTATTGCCAACAAGAAGCTTTTTGTCTCCACTAGAGACTGACGAAGAAATTGAAGTT
GTAATCGAACAAGGTAAAACGCTAATTATCAAGCTACAGGCTGTGGGTGATTTGAACAAA
AAGACCGGTGAAAGAGAAGTTTACTTTGATTTGAATGGTGAAATGAGAAAAATTCGTGTT
GCTGACAGATCACAAAAAGTGGAAACTGTTACTAAATCCAAAGCAGACATGCATGATCCA
TTACACATTGGTGCACCAATGGCAGGTGTCATTGTTGAAGTTAAAGTTCATAAAGGATCA
CTAATAAAGAAGGGCCAACCTGTAGCCGTATTAAGCGCCATGAAAATGGAAATGATTATA
TCTTCTCCATCCGATGGACAAGTTAAAGAAGTGTTTGTCTCTGATGGTGAAAATGTGGAC
TCTTCTGATTTATTAGTTCTATTAGAAGACCAAGTTCCTGTTGAAACTAAGGCATAG

Protein sequence predicted based on nucleotide sequence

MSQRKFAGLRDNFNLLGEKNKILVANRGEIPIRIFRTAHELSMQTVAIYSHEDRLSTHKQ
KADEAYVIGEVGQYTPVGAYLAIDEIISIAQKHQVDFIHPGYGFLSENSEFADKVVKAGI
TWIGPPAEVIDSVGDKVSARNLAAKANVPTVPGTPGPIETVEEALDFVNEYGYPVIIKAA
FGGGGRGMRVVREGDDVADAFQRATSEARTAFGNGTCFVERFLDKPKHIEVQLLADNHGN
VVHLFERDCSVQRRHQKVVEVAPAKTLPREVRDAILTDAVKLAKECGYRNAGTAEFLVDN
QNRHYFIEINPRIQVEHTITEEITGIDIVAAQIQIAAGASLPQLGLFQDKITTRGFAIQC
RITTEDPAKNFQPDTGRIEVYRSAGGNGVRLDGGNAYAGTIISPHYDSMLVKCSCSGSTY
EIVRRKMIRALIEFRIRGVKTNIPFLLTLLTNPVFIEGTYWTTFIDDTPQLFQMVSSQNR
AQKLLHYLADVAVNGSSIKGQIGLPKLKSNPSVPHLHDAQGNVINVTKSAPPSGWRQVLL
EKGPAEFARQVRQFNGTLLMDTTWRDAHQSLLATRVRTHDLATIAPTTAHALAGAFALEC
WGGATFDVAMRFLHEDPWERLRKLRSLVPNIPFQMLLRGANGVAYSSLPDNAIDHFVKQA
KDNGVDIFRVFDALNDLEQLKVGVDAVKKAGGVVEATVCFSGDMLQPGKKYNLDYYLEIA
EKIVQMGTHILGIKDMAGTMKPAAAKLLIGSLRAKYPDLPIHVHTHDSAGTAVASMTACA
LAGADVVDVAINSMSGLTSQPSINALLASLEGNIDTGINVEHVRELDAYWAEMRLLYSCF
EADLKGPDPEVYQHEIPGGQLTNLLFQAQQLGLGEQWAETKRAYREANYLLGDIVKVTPT
SKVVGDLAQFMVSNKLTSDDVRRLANSLDFPDSVMDFFEGLIGQPYGGFPEPFRSDVLRN
KRRKLTCRPGLELEPFDLEKIREDLQNRFGDVDECDVASYNMYPRVYEDFQKMRETYGDL
SVLPTRSFLSPLETDEEIEVVIEQGKTLIIKLQAVGDLNKKTGEREVYFDLNGEMRKIRV
ADRSQKVETVTKSKADMHDPLHIGAPMAGVIVEVKVHKGSLIKKGQPVAVLSAMKMEMII
SSPSDGQVKEVFVSDGENVDSSDLLVLLEDQVPVETKA*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3537
Mutations:
None
Discrepancy:
c1783g,g1784c,R595A
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable