NEW1 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH148235.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00008803
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3591nts
Open reading frame: 1 to 3591

ATGCCTCCAAAGAAGTTTAAGGATCTAAACTCTTTCCTTGACGACCAACCAAAGGACCCA
AATCTAGTCGCTTCTCCCTTCGGTGGTTATTTTAAAAACCCTGCTGCTGATGCCGGTAGT
AATAACGCTTCCAAAAAAAGTAGCTACCAACAACAGCGTAATTGGAAACAGGGGGGCAAC
TACCAACAAGGTGGTTACCAATCGTATAACAGTAATTACAACAATTACAACAACTACAAC
AACTACAACAATTATAACAACTACAATAACTATAATAAATACAATGGCCAGGGCTATCAA
AAATCTACTTACAAACAATCGGCTGTGACGCCCAACCAAAGTGGAACTCCAACTCCATCT
GCTTCTACTACATCTTTGACCTCTTTGAATGAAAAGCTGTCGAACTTAGAGTTAACACCC
ATCTCTCAATTTTTGTCAAAGATACCTGAATGTCAAAGTATCACTGATTGCAAAAATCAA
ATCAAACTAATCATTGAAGAATTTGGCAAAGAAGGTAACAGTACGGGTGAGAAAATTGAA
GAGTGGAAAATCGTCGATGTTCTATCTAAATTCATCAAACCTAAGAATCCATCATTAGTT
AGAGAATCTGCCATGCTAATAATTTCCAACATTGCTCAATTTTTCAGTGGAAAGCCACCA
CAGGAAGCATACTTGCTACCATTCTTCAATGTCGCACTTGACTGTATTTCAGATAAAGAG
AATACAGTCAAACGTGCCGCGCAACATGCCATTGATTCTTTGTTGAATTGCTTTCCAATG
GAGGCTTTAACCTGTTTTGTACTGCCTACGATTTTGGATTACTTATCATCTGGTGCCAAA
TGGCAAGCTAAAATGGCCGCTCTAAGCGTTGTAGATAGAATAAGAGAAGATTCGGCGAAC
GATTTATTGGAACTTACTTTCAAAGATGCAGTTCCAGTTTTAACCGATGTAGCAACTGAT
TTCAAACCTGAATTGGCCAAACAAGGTTACAAAACTTTATTGGACTACGTTTCTATTTTG
GATAACTTAGATTTGTCTCCTCGTTATAAATTGATCGTGGACACCCTGCAAGATCCATCT
AAGGTTCCTGAATCTGTAAAATCGTTATCCAGTGTTACATTTGTCGCTGAAGTTACGGAA
CCTTCACTATCTTTGTTAGTTCCTATTCTAAACAGATCTTTAAACCTATCGTCATCATCT
CAAGAGCAATTAAGACAAACCGTTATTGTCGTGGAGAATTTGACAAGATTGGTGAATAAT
CGTAATGAAATTGAAAGCTTCATCCCTCTACTACTACCCGGTATCCAAAAGGTTGTTGAT
ACTGCGTCCTTACCTGAAGTTCGTGAATTGGCTGAAAAGGCCCTTAACGTTCTAAAAGAA
GATGACGAAGCTGATAAGGAAAACAAATTCTCAGGCAGACTGACTTTAGAAGAAGGTAGG
GATTTCTTACTTGATCACCTCAAGGACATTAAAGCTGATGATTCCTGTTTTGTCAAGCCT
TACATGAATGACGAAACTGTTATCAAGTATATGAGCAAGATTTTGACCGTGGATTCTAAT
GTGAACGACTGGAAAAGACTTGAAGATTTTTTGACCGCCGTTTTTGGTGGTTCAGATTCA
CAAAGAGAATTTGTCAAACAAGACTTTATCCATAACTTGAGAGCTTTATTCTACCAAGAA
AAGGAAAGGGCAGATGAGGACGAAGGTATTGAGATTGTCAACACTGATTTCTCCTTAGCT
TATGGTTCAAGAATGCTTTTGAACAAAACAAATCTTCGTCTCCTAAAGGGTCATCGTTAC
GGTTTGTGTGGTAGAAATGGTGCTGGTAAGTCTACTTTAATGAGAGCAATTGCTAATGGT
CAATTGGATGGTTTTCCTGACAAGGATACATTACGCACCTGTTTCGTCGAACATAAATTG
CAAGGTGAAGAAGGTGATTTAGACTTGGTTTCCTTCATTGCTTTAGATGAAGAACTGCAA
TCTACTTCTCGTGAGGAAATTGCAGCCGCTTTAGAATCTGTCGGTTTTGATGAAGAGAGA
AGAGCACAAACTGTTGGATCTTTATCTGGTGGTTGGAAAATGAAGTTGGAATTGGCAAGA
GCTATGTTACAAAAAGCTGATATTTTATTGCTGGATGAACCTACCAATCATTTAGATGTT
TCGAACGTTAAATGGTTAGAAGAATACTTACTGGAACATACTGATATTACTTCATTGATT
GTTTCGCATGACTCTGGTTTCTTAGATACAGTCTGTACTGACATCATCCATTATGAAAAC
AAGAAATTGGCTTACTACAAAGGTAACTTGGCAGCATTTGTTGAGCAGAAACCTGAAGCT
AAATCTTACTATACCTTAACAGATTCTAATGCTCAAATGCGTTTCCCACCCCCAGGTATT
TTAACAGGTGTTAAGTCTAACACTAGAGCCGTCGCTAAAATGACTGATGTAACTTTCTCT
TATCCAGGTGCCCAAAAGCCTTCCTTAAGCCATGTCTCCTGTTCATTGTCTCTGTCTTCT
CGTGTGGCTTGTTTAGGTCCTAACGGTGCTGGTAAATCCACTTTGATCAAGCTATTAACT
GGTGAATTGGTTCCAAATGAAGGTAAAGTGGAAAAACATCCAAATTTACGTATTGGTTAT
ATTGCTCAGCATGCATTGCAACACGTCAATGAACATAAGGAAAAGACGGCAAACCAATAT
TTGCAATGGCGTTATCAATTCGGTGACGACCGTGAAGTCTTATTGAAGGAATCCAGAAAA
ATATCCGAAGATGAAAAGGAAATGATGACAAAGGAAATCGACATTGATGACGGTAGAGGT
AAGAGAGCCATTGAAGCTATTGTAGGTAGACAAAAGTTGAAAAAATCTTTCCAATATGAG
GTCAAATGGAAATACTGGAAACCAAAATACAACTCCTGGGTTCCAAAAGATGTTTTGGTC
GAGCATGGCTTTGAAAAATTGGTTCAAAAATTCGACGATCACGAAGCCTCCAGAGAAGGG
TTGGGTTACCGTGAATTAATTCCTTCAGTTATCACCAAACATTTTGAAGACGTTGGTCTT
GATTCTGAAATTGCTAACCATACTCCATTAGGTTCTTTATCTGGTGGTCAATTAGTTAAA
GTCGTTATTGCCGGTGCTATGTGGAACAACCCTCATTTACTTGTTTTGGATGAACCTACC
AACTATTTGGACAGAGACTCTCTTGGTGCTTTGGCCGTTGCTATTCGTGACTGGAGTGGT
GGTGTCGTTATGATTTCGCATAATAACGAATTTGTTGGTGCTTTATGTCCTGAACAATGG
ATTGTGGAGAACGGTAAAATGGTTCAAAAGGGCTCTGCACAAGTTGACCAATCCAAATTC
GAAGATGGTGGTAATGCTGATGCCGTTGGTCTAAAGGCCTCCAACTTGGCCAAGCCATCT
GTAGATGACGATGATTCTCCAGCTAACATCAAGGTCAAGCAAAGAAAGAAGAGATTGACA
AGAAATGAAAAGAAGCTGCAAGCCGAACGTCGTCGTCTACGTTACATTGAGTGGTTGTCA
TCGCCAAAAGGTACACCAAAACCAGTTGATACTGACGATGAAGAAGATTAG

Protein sequence predicted based on nucleotide sequence

MPPKKFKDLNSFLDDQPKDPNLVASPFGGYFKNPAADAGSNNASKKSSYQQQRNWKQGGN
YQQGGYQSYNSNYNNYNNYNNYNNYNNYNNYNKYNGQGYQKSTYKQSAVTPNQSGTPTPS
ASTTSLTSLNEKLSNLELTPISQFLSKIPECQSITDCKNQIKLIIEEFGKEGNSTGEKIE
EWKIVDVLSKFIKPKNPSLVRESAMLIISNIAQFFSGKPPQEAYLLPFFNVALDCISDKE
NTVKRAAQHAIDSLLNCFPMEALTCFVLPTILDYLSSGAKWQAKMAALSVVDRIREDSAN
DLLELTFKDAVPVLTDVATDFKPELAKQGYKTLLDYVSILDNLDLSPRYKLIVDTLQDPS
KVPESVKSLSSVTFVAEVTEPSLSLLVPILNRSLNLSSSSQEQLRQTVIVVENLTRLVNN
RNEIESFIPLLLPGIQKVVDTASLPEVRELAEKALNVLKEDDEADKENKFSGRLTLEEGR
DFLLDHLKDIKADDSCFVKPYMNDETVIKYMSKILTVDSNVNDWKRLEDFLTAVFGGSDS
QREFVKQDFIHNLRALFYQEKERADEDEGIEIVNTDFSLAYGSRMLLNKTNLRLLKGHRY
GLCGRNGAGKSTLMRAIANGQLDGFPDKDTLRTCFVEHKLQGEEGDLDLVSFIALDEELQ
STSREEIAAALESVGFDEERRAQTVGSLSGGWKMKLELARAMLQKADILLLDEPTNHLDV
SNVKWLEEYLLEHTDITSLIVSHDSGFLDTVCTDIIHYENKKLAYYKGNLAAFVEQKPEA
KSYYTLTDSNAQMRFPPPGILTGVKSNTRAVAKMTDVTFSYPGAQKPSLSHVSCSLSLSS
RVACLGPNGAGKSTLIKLLTGELVPNEGKVEKHPNLRIGYIAQHALQHVNEHKEKTANQY
LQWRYQFGDDREVLLKESRKISEDEKEMMTKEIDIDDGRGKRAIEAIVGRQKLKKSFQYE
VKWKYWKPKYNSWVPKDVLVEHGFEKLVQKFDDHEASREGLGYRELIPSVITKHFEDVGL
DSEIANHTPLGSLSGGQLVKVVIAGAMWNNPHLLVLDEPTNYLDRDSLGALAVAIRDWSG
GVVMISHNNEFVGALCPEQWIVENGKMVQKGSAQVDQSKFEDGGNADAVGLKASNLAKPS
VDDDDSPANIKVKQRKKRLTRNEKKLQAERRRLRYIEWLSSPKGTPKPVDTDDEED*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3591
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable