RGA2 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH148065.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00008807
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3030nts
Open reading frame: 1 to 3030

ATGTCAGCTGACCCTATTAATGACCAATCGTCTTTATGTGTGAGGTGCAATAAATCAATC
GCGTCTAGCCAGGTATACGAATTAGAAAGCAAAAAATGGCATGACCAGTGCTTTACATGC
TACAAATGTGACAAAAAATTGAACGCAGATTCAGACTTTTTGGTTCTGGATATAGGTACA
TTAATTTGTTACGACTGCTCAGATAAATGTACAAATTGTGGCGATAAAATAGACGACACA
GCAATTATACTGCCTTCCTCAAATGAGGCCTATTGTTCCAATTGTTTCAGATGCTGTCGG
TGCAGCAACCGTATAAAGAACTTAAAGTATGCAAAGACAAAACGAGGACTATGTTGTATG
GATTGTCACGAGAAATTGTTGAGAAAAAAACAACTATTACTGGAAAATCAAACAAAAAAT
TCATCGAAAGAAGATTTTCCCATAAAACTTCCTGAAAGATCAGTCAAAAGGCCCTTGTCA
CCCACCAGAATAAATGGTAAGTCAGATGTCAGTACAAACAACACCGCAATAAGTAAGAAC
CTTGTAAGCTCGAATGAGGATCAACAGCTCACTCCACAAGTTTTAGTATCACAAGAAAGA
GATGAATCATCGTTGAATGACAATAATGATAATGATAACAGCAAGGACAGGGAGGAAACA
TCTTCTCACGCAAGGACGGTTTCTATTGACGACATTCTGAACTCTACTTTGGAACACGAT
AGTAATAGCATTGAAGAGCAGAGTTTGGTGGATAATGAGGACTACATCAACAAAATGGGT
GAAGACGTAACCTATAGGCTATTAAAACCACAAAGAGCAAATCGAGATTCCATAGTGGTA
AAGGATCCGAGGATCCCAAATTCGAACTCGAACGCGAATAGATTCTTTTCAATTTATGAT
AAAGAAGAGACAGACAAAGATGATACAGACAACAAAGAGAATGAAATCATCGTCAACACA
CCCAGAAATAGCACTGATAAAATTACAAGTCCTTTGAACAGCCCTATGGCTGTACAAATG
AACGAAGAGGTCGAACCTCCGCATGGACTGGCATTAACTTTATCAGAAGCTACGAAAGAA
AATAACAAATCATCTCAAGGTATTCAAACGTCAACTTCCAAATCTATGAATCATGTCTCC
CCGATTACAAGAACCGATACCGTAGAAATGAAAACATCAACCTCTTCATCGACATTACGT
TTGTCTGATAATGGAAGTTTCAGCAGGCCTCAAACAGCAGATAACTTATTGCCGCATAAA
AAAGTAGCACCCTCTCCAAACAAAAAACTTTCAAGATCGTTTTCTTTGAAGTCCAAAAAT
TTTGTTCACAACCTGAAAAGTAAAACTTCGGAAATGCTGGACCCAAAGCATCCCCATCAC
AGCACTTCTATACAGGAATCAGACACTCATTCTGGCTGGGGTGTTTCCTCTACTCATACA
AATATAAGAAAATCAAAAGCCAAAAAAAATCCTGTATCAAGAGGCCAAAGTGACAGTACA
ATATACAATACTCTACCACAACATGGAAATTTTACAGTACCGGAGTTTAATCACAAAAAA
GCGCAAAGCAGCCTTGGCAGTATATCCAAAAAGCAAAATTCTAATGATACAGCCACTAAT
AGACGCATAAATGGTTCATTTACAAGTTCAAGCTCGGGGCATCATATAGCAATGTTCCGT
ACGCCGCCTTTGGAATCAGGGCCATTATTTAAGAGGCCATCTCTGTCATCTGAGTCCGCA
CACCACAGATCTTCTAGCTTACAGACGTCTAGATCCACTAATGCATTGTTGGAAGACGAT
TCCACGAAAGTGGATGCTACTGATGAAAGCGCTACAAGCTTGGAAAAGGACTTTTACTTT
ACTGAGTTGACTTTAAGGAAGTTAAAATTAGATGTGAGGGAACTAGAAGGTACGAAGAAA
AAATTATTACAAGATGTTGAAAATCTAAGATTAGCGAAAGAGAGGCTGTTAAATGATGTT
GACAATTTAACAAGAGAAAAGGATAAGCAATCTGCATCTTCCCGGGAGTCCTTAGAACAA
AAAGAAAACATTGCAACATCAATCACTGTAAAGTCACCAAGTTCAAATTCCGACAGAAAA
GGGAGCATATCTAATGCCAGTCCTAAACCACGCTTCTGGAAAATATTTTCAAGTGCTAAG
GATCACCAGGTAGGCGATCTAGAATCACAGCAACGTTCGCCAAATTCATCTTCAGGCGGA
ACGACAAATATAGCACAGAAAGAAATATCATCACCGAAATTAATTCGAGTACACGATGAG
CTTCCTTCTCCAGGCAAAGTACCACTGTCGCCAAGTCCAAAGAGATTAGATTATACGCCT
GATGGTTCCCATTTATATGGATCTTCGCTACAAGCTCGATGTGCGTATGAAAAAAGTACT
GTTCCAATAATTATCAGGTGCTGCATTGATCGCATTGAAAAAGATGATATAGGCCTGAAC
ATGGAAGGCTTATACAGAAAGTCGGGCTCACAGACTTTGGTTGAGGAGATCGAAAATGAA
TTCGCTCAGAATAATTCGTTGCATAGTGATACATTAAGTCCCAAACTGAATGCTTTACTG
AATCAAGATATTCATGCAGTGGCTAGCGTATTAAAACGATATTTGAGAAAACTACCAGAT
CCAGTTTTATCATTTTCCATATACGATGCCTTGATAGATCTAGTTAGAAATAACCAACTA
ATTGAGAGGCTACCATTAAACAACGACAAGTTTTTGGATTCACCACAAAAAGTGACTATT
TATGAAATGGTGCTCAAAAGCCTACTTGAAATTTTCAAAATTTTACCCGTAGAGCATCAG
GAAGTATTGAAAGTATTGGCAGCACATATCGGCAAAGTTCGAAGGTGCAGTGAAAGAAAT
TTAATGAACTTACATAACTTGTCGTTGGTCTTTGCACCGAGTCTTATCCACGATTTTGAC
GGCGAGAAAGATATTGTGGACATGAAGGAGAGGAATTATATCGTGGAATTTATACTTGGA
AACTATAGAGACACATTTAAGCAAGCATAG

Protein sequence predicted based on nucleotide sequence

MSADPINDQSSLCVRCNKSIASSQVYELESKKWHDQCFTCYKCDKKLNADSDFLVLDIGT
LICYDCSDKCTNCGDKIDDTAIILPSSNEAYCSNCFRCCRCSNRIKNLKYAKTKRGLCCM
DCHEKLLRKKQLLLENQTKNSSKEDFPIKLPERSVKRPLSPTRINGKSDVSTNNTAISKN
LVSSNEDQQLTPQVLVSQERDESSLNDNNDNDNSKDREETSSHARTVSIDDILNSTLEHD
SNSIEEQSLVDNEDYINKMGEDVTYRLLKPQRANRDSIVVKDPRIPNSNSNANRFFSIYD
KEETDKDDTDNKENEIIVNTPRNSTDKITSPLNSPMAVQMNEEVEPPHGLALTLSEATKE
NNKSSQGIQTSTSKSMNHVSPITRTDTVEMKTSTSSSTLRLSDNGSFSRPQTADNLLPHK
KVAPSPNKKLSRSFSLKSKNFVHNLKSKTSEMLDPKHPHHSTSIQESDTHSGWGVSSTHT
NIRKSKAKKNPVSRGQSDSTIYNTLPQHGNFTVPEFNHKKAQSSLGSISKKQNSNDTATN
RRINGSFTSSSSGHHIAMFRTPPLESGPLFKRPSLSSESAHHRSSSLQTSRSTNALLEDD
STKVDATDESATSLEKDFYFTELTLRKLKLDVRELEGTKKKLLQDVENLRLAKERLLNDV
DNLTREKDKQSASSRESLEQKENIATSITVKSPSSNSDRKGSISNASPKPRFWKIFSSAK
DHQVGDLESQQRSPNSSSGGTTNIAQKEISSPKLIRVHDELPSPGKVPLSPSPKRLDYTP
DGSHLYGSSLQARCAYEKSTVPIIIRCCIDRIEKDDIGLNMEGLYRKSGSQTLVEEIENE
FAQNNSLHSDTLSPKLNALLNQDIHAVASVLKRYLRKLPDPVLSFSIYDALIDLVRNNQL
IERLPLNNDKFLDSPQKVTIYEMVLKSLLEIFKILPVEHQEVLKVLAAHIGKVRRCSERN
LMNLHNLSLVFAPSLIHDFDGEKDIVDMKERNYIVEFILGNYRDTFKQA*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3030
Mutations:
None
Discrepancy:
t3014c,I1005T
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable