MIP1 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH148295.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00008830
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3843nts
Open reading frame: 1 to 3843

ATGGACTACGAAAGAACGGTTCTAAAGAAGAGGTCGAGATGGGGATTATATGTAGTTGTT
GAGCAACGAGGGACAAGTATGACGAAATTGATGGTTAGATCTGAATGCATGCTGCGAATG
GTGCGGCGGCGGCCGCTGCGTGTGCAGTTTTGTGCTCGATGGTTCTCCACAAAGAAGAAT
ACCGCAGAAGCACCCAGGATTAATCCTGTGGGGATACAGTATTTAGGTGAGTCTTTGCAA
AGACAGGTATTTGGTAGTTGCGGTGGTAAAGATGAGGTGGAGCAAAGCGACAAACTTATG
GAGTTATCGAAAAAGTCGCTAAAGGACCATGGGTTGTGGGGGAAGAAGACGCTCATCACG
GATCCAATATCGTTTCCTCTGCCGCCACTACAAGGCAGGTCGCTAGATGAGCATTTCCAG
AAGATTGGGCGTTTCAATTCTGAACCGTACAAGAGTTTTTGCGAGGACAAGTTCACGGAG
ATGGTGGCACGACCGGCGGAATGGCTGCGGAAGCCAGGCTGGGTTAAATACGTACCTGGA
ATGGCACCCGTCGAAGTGGCATACCCAGACGAAGAGCTGGTGGTGTTTGATGTAGAAACA
CTCTATAACGTCTCTGACTATCCGACTTTGGCCACGGCCTTGTCATCGACGGCGTGGTAC
CTTTGGTGTTCGCCGTTCATATGTGGTGGTGATGATCCTGCTGCACTGATACCCCTAAAC
ACATTGAATAAAGAGCAAGTAATAATTGGTCACAATGTTGCGTATGATAGGGCACGGGTT
CTCGAAGAATACAACTTCAGGGACTCGAAGGCGTTTTTCCTCGACACTCAATCTCTGCAT
ATTGCATCTTTCGGGCTGTGTTCAAGACAACGTCCGATGTTCATGAAGAATAACAAGAAA
AAAGAGGCAGAAGTGGAATCGGAAGTACATCCAGAAATTTCCATAGAAGATTATGACGAC
CCTTGGTTAAATGTGTCTGCTTTAAACTCGCTAAAGGATGTAGCGAAATTTCACTGCAAG
ATAGATCTTGATAAGACTGATAGAGATTTCTTTGCTTCCACAGATAAATCAACGATAATA
GAAAATTTCCAAAAACTGGTCAACTACTGCGCCACTGACGTAACGGCCACAAGCCAAGTG
TTTGATGAGATTTTCCCTGTTTTCCTGAAAAAGTGTCCTCATCCAGTTTCGTTTGCAGGT
TTAAAGTCCTTAAGCAAATGCATTCTGCCGACGAAGCTAAACGACTGGAATGATTACTTG
AACAGCTCTGAGTCTTTGTACCAACAGTCCAAAGTCCAGATAGAATCCAAAATTGTTCAA
ATCATAAAAGATATCGTCCTGTTGAAGGACAAACCAGATTTTTATCTCAAGGATCCCTGG
TTATCACAATTGGATTGGACCACCAAACCTTTAAGATTGACAAAGAAAGGTGTTCCTGCA
AAGTGTCAAAAGCTGCCCGGCTTTCCAGAATGGTATAGACAACTTTTTCCCTCAAAGGAC
ACTGTAGAACCTAAGATCACTATTAAATCAAGAATAATTCCGATTTTGTTCAAGTTATCG
TGGGAGAATTCCCCCGTAATATGGTCGAAGGAGTCTGGATGGTGCTTCAACGTGCCCCAT
GAACAAGTAGAAACATACAAGGCTAAAAATTATGTTTTGGCAGATAGCGTATCTCAAGAG
GAAGAGGAAATAAGAACGCATAACCTAGGATTGCAATGCACAGGTGTTCTTTTCAAAGTA
CCTCATCCCAATGGGCCGACTTTTAATTGCACTAATCTTTTAACCAAATCGTATAACCAT
TTTTTTGAAAAGGGCGTACTAAAATCTGAGTCAGAATTGGCTCATCAGGCTTTACAAATT
AACTCTTCAGGGTCTTATTGGATGTCAGCAAGGGAAAGAATCCAATCCCAATTTGTAGTC
CCCAGTTGTAAATTCCCTAATGAGTTCCAGTCTTTGTCCGCAAAATCGTCGTTAAATAAT
GAGAAGACAAATGATCTTGCCATAATTATACCGAAAATCGTCCCCATGGGCACAATCACT
AGAAGAGCTGTGGAAAATGCGTGGTTAACCGCATCCAATGCAAAAGCAAATAGGATAGGT
TCCGAATTGAAAACTCAAGTCAAAGCTCCTCCAGGTTATTGTTTTGTTGGGGCGGACGTG
GATAGTGAGGAATTGTGGATAGCATCTTTAGTCGGTGACTCCATTTTCAATGTTCATGGT
GGTACCGCCATTGGTTGGATGTGTTTAGAAGGCACTAAAAACGAAGGTACAGATTTGCAC
ACGAAGACTGCTCAAATTTTGGGGTGTTCTCGTAATGAGGCGAAAATTTTTAATTATGGT
AGAATTTACGGCGCTGGTGCTAAATTTGCGAGTCAGTTACTTAAAAGGTTTAACCCATCT
CTAACTGATGAAGAGACAAAAAAAATTGCGAATAAGTTATATGAAAATACGAAAGGTAAA
ACGAAAAGATCAAAATTATTCAAAAAATTTTGGTATGGTGGATCTGAGTCAATTCTATTC
AATAAATTAGAAAGCATCGCGGAACAAGAAACTCCAAAGACACCGGTACTGGGATGTGGT
ATTACCTATTCACTTATGAAGAAAAATCTGAGGGCGAATTCCTTTCTACCTTCAAGAATC
AATTGGGCCATCCAGTCATCTGGAGTGGATTATCTACATCTTCTTTGTTGCTCCATGGAA
TATATTATTAAGAAATATAACCTTGAGGCAAGGCTTTGCATTTCCATCCATGATGAGATT
AGATTTTTGGTGAGCGAAAAGGACAAATACAGAGCTGCTATGGCTTTGCAAATCAGCAAC
ATATGGACGAGAGCAATGTTTTGCCAGCAAATGGGGATAAATGAATTACCGCAAAACTGT
GCCTTTTTCTCGCAAGTAGATATTGATTCTGTCATACGTAAAGAAGTCAATATGGACTGC
ATAACCCCCTCGAACAAAACCGCCATTCCTCATGGGGAGGCGCTTGATATCAATCAACTG
CTAGACAAATCCAATAGTAAATTGGGTAAACCAAATCTCGATATCGACAGCAAAGTATCA
CAATATGCCTATAACTACAGAGAACCTGTATTTGAAGAATATAATAAATCTTATACTCCA
GAGTTCTTAAAATATTTTCTTGCGATGCAAGTCCAGTCAGATAAGCGCGATGTGAATCGG
CTAGAAGATGAGTATCTGCGGGAGTGTACATCCAAAGAATACGCTAGAGATGGGAACACT
GCAGAGTACAGCCTCCTAGACTATATAAAGGATGTCGAGAAGGGCAAAAGGACTAAAGTA
CGTATTATGGGATCCAATTTTTTAGATGGTACCAAAAATGCCAAAGCTGATCAACGAATC
AGATTACCTGTCAATATGCCAGATTATCCCACACTTCATAAAATTGCTAACGATTCAGCA
ATCCCAGAAAAGCAATTGCTGGAGAACAGGAGAAAGAAGGAAAACAGGATAGATGACGAA
AATAAGAAAAAATTAACCAGAAAAAAGAATACCACGCCTATGGAAAGGAAGTACAAAAGA
GTGTATGGTGGCAGGAAGGCATTTGAAGCGTTCTATGAATGTGCAAATAAACCGTTAGAC
TATACTTTGGAGACAGAAAAACAATTCTTTAATATTCCTATAGATGGAGTGATTGACGAC
GTTCTGAACGACAAATCTAATTATAAAAAGAAACCATCACAAGCAAGAACGGCATCATCA
TCGCCAATAAGAAAGACTGCGAAAGCAGTACATTCCAAAAAATTGCCGGCAAGGAAGTCA
AGCACTACAAATAGAAATTTGGTTGAGCTGGAAAGGGACATTACTATTTCTAGAGAGTAC
TAG

Protein sequence predicted based on nucleotide sequence

MDYERTVLKKRSRWGLYVVVEQRGTSMTKLMVRSECMLRMVRRRPLRVQFCARWFSTKKN
TAEAPRINPVGIQYLGESLQRQVFGSCGGKDEVEQSDKLMELSKKSLKDHGLWGKKTLIT
DPISFPLPPLQGRSLDEHFQKIGRFNSEPYKSFCEDKFTEMVARPAEWLRKPGWVKYVPG
MAPVEVAYPDEELVVFDVETLYNVSDYPTLATALSSTAWYLWCSPFICGGDDPAALIPLN
TLNKEQVIIGHNVAYDRARVLEEYNFRDSKAFFLDTQSLHIASFGLCSRQRPMFMKNNKK
KEAEVESEVHPEISIEDYDDPWLNVSALNSLKDVAKFHCKIDLDKTDRDFFASTDKSTII
ENFQKLVNYCATDVTATSQVFDEIFPVFLKKCPHPVSFAGLKSLSKCILPTKLNDWNDYL
NSSESLYQQSKVQIESKIVQIIKDIVLLKDKPDFYLKDPWLSQLDWTTKPLRLTKKGVPA
KCQKLPGFPEWYRQLFPSKDTVEPKITIKSRIIPILFKLSWENSPVIWSKESGWCFNVPH
EQVETYKAKNYVLADSVSQEEEEIRTHNLGLQCTGVLFKVPHPNGPTFNCTNLLTKSYNH
FFEKGVLKSESELAHQALQINSSGSYWMSARERIQSQFVVPSCKFPNEFQSLSAKSSLNN
EKTNDLAIIIPKIVPMGTITRRAVENAWLTASNAKANRIGSELKTQVKAPPGYCFVGADV
DSEELWIASLVGDSIFNVHGGTAIGWMCLEGTKNEGTDLHTKTAQILGCSRNEAKIFNYG
RIYGAGAKFASQLLKRFNPSLTDEETKKIANKLYENTKGKTKRSKLFKKFWYGGSESILF
NKLESIAEQETPKTPVLGCGITYSLMKKNLRANSFLPSRINWAIQSSGVDYLHLLCCSME
YIIKKYNLEARLCISIHDEIRFLVSEKDKYRAAMALQISNIWTRAMFCQQMGINELPQNC
AFFSQVDIDSVIRKEVNMDCITPSNKTAIPHGEALDINQLLDKSNSKLGKPNLDIDSKVS
QYAYNYREPVFEEYNKSYTPEFLKYFLAMQVQSDKRDVNRLEDEYLRECTSKEYARDGNT
AEYSLLDYIKDVEKGKRTKVRIMGSNFLDGTKNAKADQRIRLPVNMPDYPTLHKIANDSA
IPEKQLLENRRKKENRIDDENKKKLTRKKNTTPMERKYKRVYGGRKAFEAFYECANKPLD
YTLETEKQFFNIPIDGVIDDVLNDKSNYKKKPSQARTASSSPIRKTAKAVHSKKLPARKS
STTNRNLVELERDITISREY*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3843
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable