Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
37984
Keyword:
intended mutation
Species:
Methylobacillus flagellatus KT
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / Yes
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
MfCD00088399
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 684nts
Open reading frame: 51 to 734

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGCTGATTG
AAATTCCGGAAGTATTGACCAAGGAACAGGTAGCGCAGTGCCGGCAGCTGATGGATGCTG
CGGAGTGGGTGGATGGCAATGTCACGTCCGGCAGCCAGTCTGCGTTGGCCAAGAATAACA
TGCAGCTGCCAGAGGGTTCTCCTGTGGCGCGGCAGGTGGGAGATTTGATCCAGGATGCGC
TGGGAATGAGTCCACTGTTCATCTCTGCAGCTTTGCCGTTGAAGGTATTTCCCCCCTTGT
TCAACCGCTATGAAGGGGGGCAGGCATTTGGTACGCATGTGGATAATGCGATTCGCCAGT
TGCGCGGCACCAATTTCCGTATCCGAAGCGACCTTTCGGCGACCTTGTTTTTTTCCGAGC
CTGAAGATTATGACGGCGGCGTACTGACGATAGAGGATACGTTCGGCATTCAGGAGGTGA
AGTTACCGGCCGGACATATGGTGCTGTACCCTTCCAGTAGTTTGCATCATGTCACGCCTG
TCACACGCGGAGTGAGGGTATCGTCGTTTTTCTGGATGCAAAGCATGATCCGCGATGATG
CGCAGCGTACGTTATTGTTCCAGCTGGACGGCAGCATTCAGGCCATCGGCGCCGAGCGCG
GAGCCGGGGATCCTGAAGTGATCCGCCTGACGGGCGTGTACCATAATCTTTTAAGAATGT
GGGCAGACAGCTAACGCGACTTAATTAATTTGGCGGATGAGAGAAGATTTTCAGCCTGAT
ACAG

Protein sequence predicted based on nucleotide sequence

MLIEIPEVLTKEQVAQCRQLMDAAEWVDGNVTSGSQSALAKNNMQLPEGSPVARQVGDLI
QDALGMSPLFISAALPLKVFPPLFNRYEGGQAFGTHVDNAIRQLRGTNFRIRSDLSATLF
FSEPEDYDGGVLTIEDTFGIQEVKLPAGHMVLYPSSSLHHVTPVTRGVRVSSFFWMQSMI
RDDAQRTLLFQLDGSIQAIGAERGAGDPEVIRLTGVYHNLLRMWADS*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 684
Mutations:
N156S

Discrepancy:
a467g,N156S;
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 389946
Recommended expression in:
Not Applicable