SPT7 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH148311.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00008840
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3999nts
Open reading frame: 1 to 3999

ATGACTGAAAGAATACCAATAAAGAATTATCAAAGAACAAATGCCAAAGCTTTACTTAAA
TTGACTGAAAAACTTTTTAACAAGAACTTTTTTGATCTCTATTTAACCTCTCAGCAATTG
GTCGTTCTTGAATACCTGCTGTCGATTTCAAGTGAAGAAGACAAACTGAAAGCATGGGAC
TATTTCTTAAAGGGAAACATAGCATTAAATGTCGAAAAATCATTTCCATTAACCCAAGAA
GAAGAACATCACGGAGCGGTCTCTCCTGCCGTTGACACACGATCAGATGATGTATCATCA
CAAACAATTAAGGACAATAACAATACTAATACCAACACCAGTATCAGCAATGAAAATCAT
GTTGAAAATGAAATTGAAGATAAAGGCGATAACGCAATAGCAAATGAAGATAATTTTGTG
AATAATGACGAAAGTGATAATGTTGAAGAAGACTTATTCAAATTAGATCTAGAGGACTTG
AAGCAGCAAATAAGCGGAACAAGGTTTATTGGAAACTTATCCTTGAAAATCAGATACGTC
TTGTGGCAGTGCGCCATAGATTATATATACTGTGATCGTAATGAGTTTGGTGATGAAAAT
GATACAGAATACACCCTATTAGATGTTGAAGAGAAGGAGGAAGAGGAAATTGGTAAAAAT
GAGAAGCCACAAAACAAAGAAGGTATTTCGAAGTTCGCCGAGGATGAAGATTACGACGAT
GAAGACGAGAACTATGATGAAGACAGTACAGACGTAAAAAATGTCGATGATCCTCCAAAA
AATCTCGATTCTATTTCCTCTTCTAATATCGAAATTGACGATGAACGACGCTTGGTGCTA
AATATCTCAATATCAAAAGAAACACTGTCAAAGTTAAAAACAAATAATGTAGAAGAAATT
ATGGGAAATTGGAACAAAATTTACCACAGTTTTGAATACGATAAAGAAACTATGATAAAG
CGATTAAAACTTGAAGAAAGCGATAAAATGATAGAGAAAGGAAAGAAGAAACGAAGTCGA
AGTGATTTAGAAGCAGCTACCGATGAACAAGATCGCGAAAATACAAATGATGAGCCAGAT
ACTAATCAAAAATTGCCCACTCCTGAAGGTTCAACATTCAGCGATACTGGGAACAAGCGC
CCCAAACAAAGTAATTTAGATTTAACAGTCAATCTAGGCATCGAAAATTTATCATTAAAG
CACCTTCTATCATCTATCCAGCAAAAAAAATCCCAATTAGGAATATCAGATTACGAATTA
AAACATCTGATTATGGATGTCAGAAAAAATCGGTCAAAATGGACATCGGATGAAAGAATT
GGGCAAGAGGAATTATACGAAGCCTGTGAAAAGGTTGTTTTGGAACTTAGAAACTACACT
GAGCATTCTACACCATTTCTGAATAAAGTGAGCAAAAGAGAAGCCCCCAATTATCATCAA
ATCATCAAAAAGTCCATGGACCTGAATACTGTTTTAAAAAAACTGAAAAGCTTTCAATAT
GACTCCAAACAAGAATTTGTAGACGATATTATGCTAATATGGAAAAATTGTTTGACCTAT
AATTCAGATCCTTCACATTTTTTGAGAGGGCATGCTATTGCTATGCAGAAGAAATCTCTT
CAGTTGATTCGCATGATTCCAAATATCACAATCCGAAACAGGGCTGATTTAGAAAAGGAA
ATTGAAGATATGGAAAAAGACAAAGACTACGAATTAGATGAGGAAGAGGAAGTTGCTGGT
TCTGGAAGAAAAGGATTGAATATGGGAGCTCATATGTTGGCCAAAGAGAATGGCAAGGTG
TCAGAAAAAGATAGCTCTAAAACCGTCAAGGATGAAGCACCAACCAATGATGACAAACTA
ACTTCTGTCATCCCTGAGGGGGAAAAAGAGAAAGATAAAACTGCTTCATCTACTGTAACG
GTACACGAAAATGTAAATAAGAACGAAATAAAAGAAAATGGGAAAAATGAAGAGCAAGAT
ATGGTTGAGGAAAGTAGTAAGACTGAGGATTCATCAAAAGATGCTGATGCTGCCAAAAAG
GATACGGAAGACGGACTACAAGATAAAACTGCAGAAAATAAGGAGGCTGGGGAAAATAAT
GAAGAGGAAGAGGATGATGATGACGAAGATGAAGACGAAGACATGGTCGACTCCCAATCT
TATTTACTTGAAAAGGATGACGATAGAGACGATTTGGAAATATCCGTGTGGAAAACTGTA
ACTGCCAAAGTTCGTGCGGAAATTTGCTTAAAAAGAACTGAATATTTTAAAAATGGAAAA
TTAAATAGTGATTCAGAGGCGTTTTTGAAAAACCCACAAAGAATGAAAAGGTTCGACCAG
CTTTTTCTTGAATATAAAGAGCAGAAAGCTTTAGAATCATATCGTCAAAAAATAGAGCAA
AATTCCATTATGAAAAATGGCTTTGGAACAGTACTAAAACAGGAAGACGATGACCAATTG
CAGTTTCATAATGATCACTCTTTAAATGGAAATGAAGCTTTTGAAAAGCAACCCAATGAT
ATTGAGTTAGATGATACCAGATTCCTACAGGAATATGATATTAGTAACGCCATTCCTGAC
ATAGTATACGAGGGAGTAAATACTAAAACATTAGACAAGATGGAAGACGCTTCCGTGGAC
CGCATGCTTCAAAATGGTATCAACAAACAAAGCAGATTTCTGGCTAACAAGGATTTAGGA
CTAACACCTAAAATGAACCAAAATATCACACTGATTCAGCAAATTAGGCACATATGCCAT
AAAATATCCCTGATCAGAATGTTACAGAGCCCTTTATCGGCTCAAAACTCCAGAAGCAAT
CCCAACGCTTTCCTTAACAACCACATTTATAATTACACTATTATTGATGACTCACTCGAT
ATTGATCCGGTGTCACAGCTTCCAACGCATGATTACAAAAACAACAGGGAGCTGATATGG
AAATTCATGCATAAGAACATATCTAAGGTTGCTATGGCCAATGGGTTTGAAACTGCCCAT
CCATCAGCAATAAACATGCTTACTGAAATCGCCGGGGATTACCTATCTAATCTGATAAAG
ACTTTGAAGCTTCATCATGAAACTAACTCCTTAAATAGAGGAACAAATGTGGAAATGCTG
CAAACAACACTGTTGGAAAACGGTATCAACAGGCCAGACGATCTATTTTCCTATGTTGAA
TCTGAATTTGGTAAAAAAACTAAGAAACTTCAGGACATCAAACAGAAACTAGAAAGCTTT
TTGAGAGCCTTATTAAGGCCAACTTTGCAGGAGTTGTCCGAGAGAAACTTTGAAGACGAG
AGCCAAAGCTTTTTTACAGGTGACTTTGCCAGCGAATTGACTGGTGAAGACTTCTTTGGT
TTTAGAGAGCTTGGATTAGAAAAGGAGTTTGGAGTTTTGAGTTCATCTGTTCCATTACAG
TTACTGACTACTCAGTTTCAAACTGTTGACGGGGAAACCAAAGTGCAGGCCAAAAAGATC
CAACCGGAAGAATCAGACAGCATTGTGTATAAGAAAATTACAAAAGGTATGCTGGATGCT
GGTTCATTCTGGAATACTCTACTTCCCCTATTACAAAAAGATTATGAACGTTCCAAGGCC
TATATAGCAAAGCAAAGCAAGTCATCTGCAAATGATAAAACCTCAATGACTTCCACAGAA
GACAATTCTTTCGCTTTACTAGAAGAGGATCAGTTTGTCTCAAAGAAAACCGCAACGAAG
GCAAGATTACCTCCTACTGGTAAGATAAGTACCACATACAAAAAGAAACCGATCGCAAGC
GCGTTTATACTTCCAGAAGAAGACTTGGAAAACGACGTAAAAGCGGATCCAACAACAACT
GTAAACGCCAAAGTGGGTGCAGAAAATGATGGAGATTCTTCCTTATTTTTGCGAACGCCT
CAACCTTTAGATCCTTTGGATATGGATGATGCTTTTGATGATACCAATATGGGCAGCAAT
AGTTCATTTAGCTTGAGCCTTCCTCGCCTTAATCAATAG

Protein sequence predicted based on nucleotide sequence

MTERIPIKNYQRTNAKALLKLTEKLFNKNFFDLYLTSQQLVVLEYLLSISSEEDKLKAWD
YFLKGNIALNVEKSFPLTQEEEHHGAVSPAVDTRSDDVSSQTIKDNNNTNTNTSISNENH
VENEIEDKGDNAIANEDNFVNNDESDNVEEDLFKLDLEDLKQQISGTRFIGNLSLKIRYV
LWQCAIDYIYCDRNEFGDENDTEYTLLDVEEKEEEEIGKNEKPQNKEGISKFAEDEDYDD
EDENYDEDSTDVKNVDDPPKNLDSISSSNIEIDDERRLVLNISISKETLSKLKTNNVEEI
MGNWNKIYHSFEYDKETMIKRLKLEESDKMIEKGKKKRSRSDLEAATDEQDRENTNDEPD
TNQKLPTPEGSTFSDTGNKRPKQSNLDLTVNLGIENLSLKHLLSSIQQKKSQLGISDYEL
KHLIMDVRKNRSKWTSDERIGQEELYEACEKVVLELRNYTEHSTPFLNKVSKREAPNYHQ
IIKKSMDLNTVLKKLKSFQYDSKQEFVDDIMLIWKNCLTYNSDPSHFLRGHAIAMQKKSL
QLIRMIPNITIRNRADLEKEIEDMEKDKDYELDEEEEVAGSGRKGLNMGAHMLAKENGKV
SEKDSSKTVKDEAPTNDDKLTSVIPEGEKEKDKTASSTVTVHENVNKNEIKENGKNEEQD
MVEESSKTEDSSKDADAAKKDTEDGLQDKTAENKEAGENNEEEEDDDDEDEDEDMVDSQS
YLLEKDDDRDDLEISVWKTVTAKVRAEICLKRTEYFKNGKLNSDSEAFLKNPQRMKRFDQ
LFLEYKEQKALESYRQKIEQNSIMKNGFGTVLKQEDDDQLQFHNDHSLNGNEAFEKQPND
IELDDTRFLQEYDISNAIPDIVYEGVNTKTLDKMEDASVDRMLQNGINKQSRFLANKDLG
LTPKMNQNITLIQQIRHICHKISLIRMLQSPLSAQNSRSNPNAFLNNHIYNYTIIDDSLD
IDPVSQLPTHDYKNNRELIWKFMHKNISKVAMANGFETAHPSAINMLTEIAGDYLSNLIK
TLKLHHETNSLNRGTNVEMLQTTLLENGINRPDDLFSYVESEFGKKTKKLQDIKQKLESF
LRALLRPTLQELSERNFEDESQSFFTGDFASELTGEDFFGFRELGLEKEFGVLSSSVPLQ
LLTTQFQTVDGETKVQAKKIQPEESDSIVYKKITKGMLDAGSFWNTLLPLLQKDYERSKA
YIAKQSKSSANDKTSMTSTEDNSFALLEEDQFVSKKTATKARLPPTGKISTTYKKKPIAS
AFILPEEDLENDVKADPTTTVNAKVGAENDGDSSLFLRTPQPLDPLDMDDAFDDTNMGSN
SSFSLSLPRLNQ*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3999
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable