COP1 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH148603.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00008866
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3606nts
Open reading frame: 1 to 3606

ATGAAGATGTTAACTAAATTTGAATCAAAGTCCACTAGGGCCAAGGGCATTGCCTTCCAC
CCCTCCAGACCTTGGGTTTTGGTGGCTTTGTTTTCCTCTACTATTCAATTATGGGATTAC
AGGATGGGAACACTACTTCACAGATTTGAAGACCACGAGGGCCCTGTTCGTGGGCTAGAT
TTCCATCCAACCCAACCGATTTTTGTTTCGGCAGGTGACGACTATACCATTAAAGTTTGG
TCCTTAGATACTAACAAATGTCTATACACCTTAACTGGGCATTTGGATTATGTCCGTACC
GTGTTTTTCCACCGTGAGCTGCCTTGGATCATATCTGCTTCTGATGACCAGACCATAAGA
ATCTGGAACTGGCAAAACCGTAAGGAAATTGCATGTTTGACCGGCCACAATCATTTTGTC
ATGTGTGCTCAATTTCATCCAACCGATGACCTGATCGTTTCCGCTTCTCTGGATGAAACC
ATCAGAATTTGGGATATTTCCGGTTTAAGGAAAAGACACTCCGCTCCTGGTACTAGTTCA
TTTGAGGAGCAAATGAGTGCACAACAAAATCTATTGGATGGCTCTCTTGGTGACTGTGTT
GTCAAGTTTATTCTAGAGGGTCACACAAGAGGTGTCAACTGGGCCTCTTTCCATCCAACT
TTACCATTGATTGTCTCCGGCAGTGACGACCGTCAAGTTAAATTATGGAGAATGAGTGCT
ACGAAAGCTTGGGAAGTTGATACTTGCAGAGGTCACACTAACAATGTTGATAGTGTTATC
TTTCACCCACATCAAAACTTGATCATTTCTGTCGGTGAAGACAAAACGCTAAGGGTTTGG
GATCTTGACAAGAGAACTCCAGTAAAGCAATTCAAAAGAGAAAACGATAGATTTTGGTTG
ATTGCAGCACATCCTCATATCAATCTATTTGGTGCAGCTCACGATTCTGGTATTATGGTT
TTCAAGCTCGATCGCGAAAGACCATGCAGCTTTATCCATCAAAACCAGTTGTTTTTTGTC
AATGCGGAAAAGCAAATCCAAAGTTTCAATTTCCAGAAAAGAGTCGCTTCATTACCTTAT
GCGTCCTTAAAGGGTATTGGTCAACCTTGGGATGCATTCAGAAGTATATCGTACAATCCC
TCCCAACATTCAGTTTTGGTTAATGAAGCTAATGGCAAATTTGCTCTCGTTATTTTACCA
AAACAACCAGTTGGAGCTGTGGAGCCAACAAGTGTCACCCAAGATACAGGTAATTTTGCT
ACTTTTGTTGGTCGTAATAGATTTGTTGTTTACAACAAGAACACTGAATCTGTTGAAGTT
CGCTCTCTCGAAAACAAAGTTACAAGAAATATCAAAGTAGAGGAAACAGTAAGAACTATT
GTAGCTGCAGGTCCTGGATCTGTTTTGGTCATACACCCCAGAGAAGTTATTCTTTATGAT
GTCCAACAGGGCAAAAAAGTATCCCAATTGGCTGTTAAAAACGTCAAGTACGTTTCGTGG
TCCTTAGATGGTCAATATGTTGCATTGATGAGTAAGCACACCATAACTTTGGCTACCAAA
AAGCTAGAATTGATTAATTCTATGCATGAAACGATTAGAATCAAAAGTGCCGCTTGGGAT
GAAACTGGTGTATTAATTTATTCAACTTTAAATCATATAAGGTACTCACTATTGAACGGG
GATAGAGGTATTATTAAAACTTTGGAGAAAACTTTATATATCACAAAAGTTCAGGGAAAA
TTAGTGTACTGCTTGAACCGTGAAGGTGAGATTGAAATTTTAACCATTGATCCTACAGAA
TATCGCTTCAAAAAGGCCCTGGTTAACAAAAATTTCCCAGAAGTTTTACGTCTGATAAAA
GATTCTAATTTGGTTGGCCAGAATATCATTTCCTATCTGCAGAAGTCAGGTTACCCAGAA
ATTGCTTTACAATTCGTTCAAGACCCACATATCCGTTTTGATTTAGCTTTGGAATACGGT
AATTTGGATGTTGCCTTAGATGAAGCCAAAAAACTGAATGATTCTAGTACTTGGGAAAGA
TTAATCCAAGAAGCCTTGGCACAAGGTAATGCCAGCTTAGCTGAAATGATTTATCAAACT
CAACATTCTTTTGACAAACTTTCCTTCCTGTATTTAGTTACAGGTGACGTTAACAAACTA
TCTAAAATGCAAAATATCGCTCAGACTCGTGAAGACTTTGGAAGTATGCTATTAAACACC
TTTTACAACAATTCAACCAAGGAAAGATCTAGCATTTTTGCAGAGGGCGGCTCTTTACCA
TTAGCTTATGCCGTAGCAAAGGCTAACGGAGATGAAGCGGCTGCTTCTGCTTTCTTGGAG
CAAGCTGAAGTTGATGAACAAGATGTTACTTTACCAGACCAGATGGATGCCTCCAACTTC
GTTCAAAGACCAGTGATCTCAAAGCCACTTGAAAAATGGCCATTAAAAGAGGCTGAACTA
TCTTACTTTGAAAAGGCTGTTTTAGGTCAAATTGACGATTTGACTATAGACGATGAAACT
CCAGCAGTAAACACCACTCAAGAGCAAGAAGAACCACTAGGCGAAGAAAACTTTAACGAT
GAGGATATTGGTGAAGACGAAGGTGCCTGGGATTTGGGTGACGAAGATTTGGATGTGGGA
GAAGAGTTACCTGAAGAAGTTGAGCAAGGCGAAATTACCTCTCCAGCACAGGAAGTTGAA
ACAGCCATTTGGATCAAGAATTCTAAACTGCCTGCTGTCCTAGTTGCCGCTGGTGCCTTT
GATGCCGCTGTTCAAGCCTTGAGCAAGCAAGTGGGTGTAGTCAAGCTGGAGCCATTGAAG
AAATATTTTACCAACATTTATGAGGGTTGTAGAACATACATTCCTAGTACGCCCTGTGAA
CTTCCTGCTCAATTAGGTTATGTCAGAGCGTATGATGATACCGTAAGTGAAGATCAAATT
CTTCCTTACGTGCCAGGTCTTGATGTCGTTAATGAAAAAATGAATGAGGGTTACAAAAAC
TTTAAACTAAACAAACCAGATATTGCAATCGAATGCTTCAGAGAAGCTATCTACCGTATT
ACGCTATTAATGGTCGATGATGCTGAAGATGAAAAATTAGCACACAAGATACTGGAAACT
GCTAGGGAGTATATATTAGGTTTATCCATTGAATTGGAACGCCGCTCATTAAAGGAGGGA
AATACTGTACGCATGCTTGAGCTAGCTGCATACTTTACAAAGGCAAAACTATCCCCAATT
CATCGTACCAATGCTTTACAAGTGGCTATGTCCCAACATTTCAAACACAAAAATTTCTTA
CAGGCCTCATATTTTGCCGGTGAATTTTTAAAGATTATTTCTTCTGGTCCTCGTGCAGAA
CAAGCACATAAAATCAAAAACAAAGCCGACTCAATGGCCAGTGACGCCATTCCAATTGAC
TTTGATCCTTATGCTAAATTCGATATTTGTGCAGCCACCTACAAACCAATTTATGAAGAT
ACACCTTCCGTTTCTGACCCATTAACAGGTAGTAAATACGTTATCACGGAAAAAGATAAA
ATAGATAGAATTGCTATGATTTCTAAGATCGGTGCACCTGCATCCGGATTAAGAATACGT
GTATAG

Protein sequence predicted based on nucleotide sequence

MKMLTKFESKSTRAKGIAFHPSRPWVLVALFSSTIQLWDYRMGTLLHRFEDHEGPVRGLD
FHPTQPIFVSAGDDYTIKVWSLDTNKCLYTLTGHLDYVRTVFFHRELPWIISASDDQTIR
IWNWQNRKEIACLTGHNHFVMCAQFHPTDDLIVSASLDETIRIWDISGLRKRHSAPGTSS
FEEQMSAQQNLLDGSLGDCVVKFILEGHTRGVNWASFHPTLPLIVSGSDDRQVKLWRMSA
TKAWEVDTCRGHTNNVDSVIFHPHQNLIISVGEDKTLRVWDLDKRTPVKQFKRENDRFWL
IAAHPHINLFGAAHDSGIMVFKLDRERPCSFIHQNQLFFVNAEKQIQSFNFQKRVASLPY
ASLKGIGQPWDAFRSISYNPSQHSVLVNEANGKFALVILPKQPVGAVEPTSVTQDTGNFA
TFVGRNRFVVYNKNTESVEVRSLENKVTRNIKVEETVRTIVAAGPGSVLVIHPREVILYD
VQQGKKVSQLAVKNVKYVSWSLDGQYVALMSKHTITLATKKLELINSMHETIRIKSAAWD
ETGVLIYSTLNHIRYSLLNGDRGIIKTLEKTLYITKVQGKLVYCLNREGEIEILTIDPTE
YRFKKALVNKNFPEVLRLIKDSNLVGQNIISYLQKSGYPEIALQFVQDPHIRFDLALEYG
NLDVALDEAKKLNDSSTWERLIQEALAQGNASLAEMIYQTQHSFDKLSFLYLVTGDVNKL
SKMQNIAQTREDFGSMLLNTFYNNSTKERSSIFAEGGSLPLAYAVAKANGDEAAASAFLE
QAEVDEQDVTLPDQMDASNFVQRPVISKPLEKWPLKEAELSYFEKAVLGQIDDLTIDDET
PAVNTTQEQEEPLGEENFNDEDIGEDEGAWDLGDEDLDVGEELPEEVEQGEITSPAQEVE
TAIWIKNSKLPAVLVAAGAFDAAVQALSKQVGVVKLEPLKKYFTNIYEGCRTYIPSTPCE
LPAQLGYVRAYDDTVSEDQILPYVPGLDVVNEKMNEGYKNFKLNKPDIAIECFREAIYRI
TLLMVDDAEDEKLAHKILETAREYILGLSIELERRSLKEGNTVRMLELAAYFTKAKLSPI
HRTNALQVAMSQHFKHKNFLQASYFAGEFLKIISSGPRAEQAHKIKNKADSMASDAIPID
FDPYAKFDICAATYKPIYEDTPSVSDPLTGSKYVITEKDKIDRIAMISKIGAPASGLRIR
V*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3606
Mutations:
None
Discrepancy:
g3368a,R1123H
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable