Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH148530.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00008872
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3282nts
Open reading frame: 1 to 3282
ATGCCATTTGATGTTTTGATATCAAACCAAAAAGATAATACCAATCACCAGAACATCACG
CCAATATCAAAATCAGTTCTTTTGGCGCCTCATTCAAACCATCCAGTGATTGAAATAGCT
ACGTATTCAGAAACCGATGTATACAAATGCTATATACGTGGGTTTGAAACTAAAATAGTG
ATGAGAAGAACTAAAGATGATTGGATTAATATTACCCAGGTTTTCAAAATAGCTCAATTT
TCGAAGACGAAAAGAACTAAAATACTGGAAAAGGAATCAAATGATATGCAACATGAAAAA
GTTCAAGGTGGGTATGGTAGGTTTCAAGGTACTTGGATCCCACTGGATTCAGCGAAGTTT
TTAGTCAATAAGTACGAAATAATTGACCCTGTGGTCAACTCCATCTTAACTTTTCAATTC
GATCCAAATAACCCTCCACCAAAGAGAAGCAAAAATAGTATATTGAGGAAGACTTCGCCG
GGAACGAAGATAACTTCTCCATCGAGCTACAATAAAACACCAAGAAAAAAGAACAGTAGT
TCATCAACATCGGCTACAACCACAGCTGCGAATAAGAAAGGCAAGAAAAATGCATCGATC
AACCAGCCGAATCCAAGTCCTTTACAAAACCTTGTTTTCCAAACTCCTCAACAGTTCCAA
GTAAATTCATCAATGAATATAATGAACAATAATGATAACCACACCACGATGAATTTCAAC
AATGACACAAGACACAATTTAATAAATAATATTAGTAACAACAGCAATCAATCTACTATT
ATTCAACAACAAAAATCCATTCATGAAAACTCTTTCAATAATAATTATTCGGCAACTCAG
AAACCTCTTCAATTTTTTCCAATTCCGACAAATTTACAAAACAAGAACGTTGCTCTGAAT
AACCCTAATAATAATGACAGTAATAGTTACAGCCATAACATCGACAATGTCATTAACAGC
AGTAATAACAATAATAATGGCAATAATAATAACTTGATCATAGTTCCAGACGGACCTATG
CAAAGCCAACAACAACAACAACATCATCATGAATATTTGACGAATAACTTCAATCATTCC
ATGATGGACTCCATTACAAATGGCAATAGTAAAAAAAGGAGGAAAAAGCTGAATCAATCT
AATGAACAACAATTTTACAACCAGCAAGAAAAAATACAAAGGCATTTCAAATTAATGAAG
CAACCCTTATTATGGCAATCCTTTCAAAATCCAAATGATCACCATAATGAGTACTGTGAC
AGTAACGGCAGCAACAATAACAACAACACTGTAGCATCCAATGGCTCCTCCATTGAAGTA
TTTTCATCAAATGAAAATGATAACAGTATGAATATGTCTTCACGATCTATGACACCGTTC
AGCGCAGGAAATACTTCATCTCAAAACAAGTTGGAAAACAAAATGACAGATCAAGAATAC
AAGCAGACGATATTAACAATACTATCATCGGAAAGGTCTTCTGATGTAGATCAAGCGTTG
TTGGCTACTTTATATCCGGCGCCCAAAAATTTTAACATAAATTTTGAAATTGATGATCAA
GGTCACACTCCACTCCATTGGGCTACAGCTATGGCGAATATTCCTTTGATAAAAATGCTA
ATTACCTTAAATGCTAATGCATTACAGTGTAATAAGCTCGGTTTTAATTGCATCACAAAA
TCAATTTTCTACAACAATTGCTACAAGGAGAATGCTTTCGATGAAATAATATCCATCTTA
AAAATATGTCTTATAACTCCCGACGTAAATGGAAGATTGCCATTTCATTATTTAATTGAA
TTGAGCGTTAATAAGTCCAAAAATCCAATGATAATAAAATCTTATATGGATTCGATAATA
TTGAGCTTGGGTCAGCAGGACTACAATTTGTTGAAGATATGTTTGAACTACCAAGATAAT
ATAGGGAACACTCCACTGCATTTATCTGCTTTAAACTTGAACTTCGAGGTTTACAACAGA
CTGGTATATCTTGGTGCGTCAACAGATATATTGAATCTAGATAACGAATCGCCTGCGTCA
ATAATGAATAAGTTTAATACTCCGGCTGGCGGGTCAAACAGCAGAAACAACAACACCAAG
GCAGATCGAAAGTTAGCTCGAAACCTTCCTCAAAAGAATTATTACCAACAACAGCAACAG
CAACAGCAACCACAAAACAATGTCAAAATTCCAAAAATTATAAAAACTCAACATCCTGAT
AAAGAAGATTCTACTGCAGATGTCAATATAGCTAAAACAGATTCTGAAGTCAACGAAAGT
CAATACCTGCATTCCAACCAGCCAAATTCTACAAATATGAACACTATTATGGAAGACTTG
TCGAATATTAATTCTTTCGTGACGTCGTCTGTCATTAAAGATATAAAGTCTACGCCGTCC
AAAATTCTTGAAAACTCTCCCATTTTATACAGAAGAAGATCACAGTCAATATCAGATGAA
AAAGAAAAGGCAAAGGATAATGAGAATCAAGTTGAAAAGAAAAAGGATCCCTTGAATAGT
GTGAAAACTGCCATGCCATCATTGGAATCGCCTTCTTCCCTTCTGCCGATACAGATGTCT
CCACTAGGAAAGTACTCAAAACCGCTTTCGCAGCAGATCAACAAATTAAATACAAAAGTT
TCTTCTCTACAAAGAATAATGGGTGAAGAAATTAAAAATCTGGACAACGAAGTAGTAGAA
ACCGAATCCTCTATTTCTAACAATAAGAAGAGATTGATTACAATAGCACATCAAATAGAG
GATGCTTTCGATTCCGTATCAAACAAAACTCCCATAAATTCGATATCAGATTTACAATCA
AGGATTAAAGAAACATCGTCAAAGTTGAATTCTGAAAAGCAAAATTTTATTCAAAGCTTG
GAAAAATCTCAAGCTTTAAAACTGGCTACAATTGTCCAGGATGAGGAATCAAAGGTTGAT
ATGAATACTAACTCAAGTTCACATCCAGAAAAACAGGAAGATGAAGAGCCAATTCCAAAG
TCAACGTCGGAAACTTCTTCTCCTAAAAACACCAAAGCGGACGCCAAATTTTCTAATACT
GTACAAGAATCATATGATGTAAATGAGACGTTGAGACTAGCAACGGAACTAACTATTCTA
CAGTTTAAAAGAAGGATGACAACATTGAAAATAAGCGAAGCTAAAAGCAAAATTAATTCA
TCGGTGAAGTTAGATAAATACAGAAACTTGATTGGTATCACAATTGAGAACATTGACTCA
AAATTGGACGATATAGAAAAGGATTTGAGGGCAAACGCATAG
Protein sequence predicted based on nucleotide sequence
MPFDVLISNQKDNTNHQNITPISKSVLLAPHSNHPVIEIATYSETDVYKCYIRGFETKIV MRRTKDDWINITQVFKIAQFSKTKRTKILEKESNDMQHEKVQGGYGRFQGTWIPLDSAKF LVNKYEIIDPVVNSILTFQFDPNNPPPKRSKNSILRKTSPGTKITSPSSYNKTPRKKNSS SSTSATTTAANKKGKKNASINQPNPSPLQNLVFQTPQQFQVNSSMNIMNNNDNHTTMNFN NDTRHNLINNISNNSNQSTIIQQQKSIHENSFNNNYSATQKPLQFFPIPTNLQNKNVALN NPNNNDSNSYSHNIDNVINSSNNNNNGNNNNLIIVPDGPMQSQQQQQHHHEYLTNNFNHS MMDSITNGNSKKRRKKLNQSNEQQFYNQQEKIQRHFKLMKQPLLWQSFQNPNDHHNEYCD SNGSNNNNNTVASNGSSIEVFSSNENDNSMNMSSRSMTPFSAGNTSSQNKLENKMTDQEY KQTILTILSSERSSDVDQALLATLYPAPKNFNINFEIDDQGHTPLHWATAMANIPLIKML ITLNANALQCNKLGFNCITKSIFYNNCYKENAFDEIISILKICLITPDVNGRLPFHYLIE LSVNKSKNPMIIKSYMDSIILSLGQQDYNLLKICLNYQDNIGNTPLHLSALNLNFEVYNR LVYLGASTDILNLDNESPASIMNKFNTPAGGSNSRNNNTKADRKLARNLPQKNYYQQQQQ QQQPQNNVKIPKIIKTQHPDKEDSTADVNIAKTDSEVNESQYLHSNQPNSTNMNTIMEDL SNINSFVTSSVIKDIKSTPSKILENSPILYRRRSQSISDEKEKAKDNENQVEKKKDPLNS VKTAMPSLESPSSLLPIQMSPLGKYSKPLSQQINKLNTKVSSLQRIMGEEIKNLDNEVVE TESSISNNKKRLITIAHQIEDAFDSVSNKTPINSISDLQSRIKETSSKLNSEKQNFIQSL EKSQALKLATIVQDEESKVDMNTNSSSHPEKQEDEEPIPKSTSETSSPKNTKADAKFSNT VQESYDVNETLRLATELTILQFKRRMTTLKISEAKSKINSSVKLDKYRNLIGITIENIDS KLDDIEKDLRANA*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3282
Mutations:
None
Discrepancy:
g145a,E49K; t561c
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
