KAP123 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH148546.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00008881
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3342nts
Open reading frame: 1 to 3342

ATGGATCAACAATTTCTAAGTCAACTTGAGCAAACTTTGCACGCTATCACTTCTGGTGTA
GGCTTGAAGGAGGCTACAAAAACTTTGCAAACTCAATTTTATACCCAGCCTACAACTTTG
CCAGCTTTGATTCATATTTTGCAGAACGGCTCCGATGATTCGTTGAAACAACTAGCTGGT
GTTGAAGCTAGAAAGTTGGTTTCCAAGCACTGGAACGCCATTGATGAATCCACTAGAGCC
TCCATCAAAACATCTCTTTTACAAACAGCATTTAGTGAGCCAAAAGAAAATGTTCGTCAT
TCCAACGCTCGAGTAATTGCTTCTATTGGTACTGAAGAGCTAGATGGTAACAAATGGCCA
GATTTAGTCCCTAACCTAATCCAAACTGCTTCTGGTGAAGACGTTCAAACAAGACAAACT
GCCATTTTTATTTTGTTTTCTCTGTTGGAAGATTTCACCTCTTCTCTATCTGGCCACATC
GATGACTTTTTGGCTTTGTTCTCTCAGACAATTAATGATCCATCTTCTTTGGAGATCAGA
TCATTATCTGCCCAGGCTTTGAACCACGTTTCTGCTTTGATTGAAGAACAAGAAACCATC
AACCCTGTTCAAGCTCAAAAATTCGCCGCCTCAATTCCATCTGTGGTTAACGTTTTAGAC
GCAGTTATCAAGGCCGACGACACCATGAACGCTAAATTAATCTTTAACTGTTTAAACGAT
TTCCTGCTATTGGACTCCCAATTAACAGGAAACTTTATTGTGGATTTGATTAAGCTGTCT
TTACAAATTGCTGTTAACAGTGAGATTGACGAAGATGTTCGAGTTTTTGCTCTTCAATTC
ATCATATCTTCATTGTCCTACAGAAAATCGAAGGTTTCTCAAAGCAAATTGGGCCCAGAA
ATTACTGTTGCAGCTTTGAAGGTGGCTTGTGAAGAGATCGATGTTGACGATGAATTAAAC
AACGAAGACGAAACAGGCGAAAATGAGGAAAATACACCCTCTTCTTCTGCTATTAGACTG
TTAGCTTTCGCTTCCTCTGAACTGCCACCATCCCAGGTTGCTTCCGTTATTGTTGAACAC
ATTCCAGCTATGTTGCAATCCGCAAACGTTTTTGAGAGAAGAGCTATTCTTTTAGCTATT
TCCGTTGCTGTTACTGGTTCTCCAGACTACATTTTGTCTCAATTCGACAAAATTATCCCT
GCCACCATAAACGGTTTGAAGGATACTGAACCGATTGTTAAATTAGCTGCTCTGAAGTGT
ATTCACCAATTAACCACTGACTTACAAGACGAAGTTGCCAAATTTCATGAAGAATACTTG
CCATTGATCATCGATATCATTGATTCTGCGAAAAACATTGTCATTTACAACTATGCAACT
GTTGCTTTAGATGGTCTGCTTGAGTTCATTGCTTATGATGCTATCGCTAAGTACTTGGAT
CCATTAATGAACAAACTATTCTATATGTTGGAAAGTAATGAATCATCCAAGTTGAGATGT
GCTGTTGTTTCTGCAATTGGTTCCGCTGCTTTTGCAGCAGGTTCCGCGTTCATTCCATAT
TTCAAGACTAGTGTTCACTATTTGGAAAAATTCATCCAAAACTGTTCTCAAATTGAAGGT
ATGAGCGAAGATGATATAGAACTAAGGGCCAACACTTTTGAAAATATTTCTACTATGGCC
AGAGCCGTCAGATCAGACGCATTTGCCGAATTTGCCGAACCTTTGGTTAACTCCGCCTAT
GAGGCCATCAAAACTGATTCTGCTAGATTAAGAGAATCTGGTTATGCATTCATTGCAAAC
TTGGCTAAAGTTTACGGCGAAAATTTTGCTCCTTTCTTGAAAACTATCCTACCAGAAATC
TTCAAGACACTAGAATTAGATGAATACCAATTCAACTTCGATGGTGATGCAGAGGATTTA
GCCGCATTTGCTGATTCTGCCAATGAAGAAGAACTACAGAACAAATTCACCGTTAACACT
GGTATTTCCTATGAAAAGGAAGTTGCTTCTGCAGCTCTATCCGAATTAGCATTGGGTACG
AAGGAGCATTTCTTGCCATACGTCGAACAATCCTTAAAGGTTTTGAATGAACAAGTCGAT
GAGTCGTACGGTTTAAGAGAAACCGCTCTAAACACCATCTGGAATGTTGTTAAGTCTGTC
CTGTTGGCTTCCAAAGTAGAACCAGAAAGCTATCCTAAGGGTATCCCAGCCTCTTCCTAC
GTTAATGCTGATGTTTTGGCGGTTATTCAAGCTGCCAGGGAAACTTCCATGGGTAACTTA
AGCGATGAATTCGAAACCTCAATGGTCATTACCGTTATGGAAGATTTTGCCAATATGATT
AAGCAATTTGGCGCAATCATCATCATGGACAACGGCGACTCTTCTATGCTAGAAGCTTTG
TGCATGCAAGTTCTTAGTGTCTTGAAGGGTACCCATACTTGTCAAACCATTGATATCGAG
GAGGACGTTCCAAGAGATGAAGAATTAGACGCTTCTGAAACCGAAGCAACTTTACAAGAT
GTTGCATTGGAAGTTCTTGTCAGTTTATCTCAAGCTTTAGCTGGTGATTTTGCCAAAGTC
TTTGACAACTTCAGACCGGTTGTTTTCGGATTATTCCAATCTAAATCCAAGAATAAGAGA
TCATCCGCCGTCGGTGCCGCCTCTGAATTAGCTCTAGGTATGAAAGAACAAAATCCTTTC
GTTCATGAAATGCTAGAAGCCTTAGTCATTAGATTAACAAGCGATAAATCTTTAGAAGTT
AGAGGTAACGCGGCTTATGGTGTTGGTTTGTTGTGTGAATATGCTTCTATGGACATCTCA
GCCGTTTATGAACCTGTTTTGAAGGCGCTTTATGAGTTACTAAGTGCTGCTGACCAAAAG
GCCTTAGCAGCCGAAGATGACGAAGCCACCAGAGAGATTATTGATAGAGCATACGCTAAT
GCTAGTGGATGTGTGGCCAGAATGGCTTTGAAGAACAGTGCTCTAGTTCCATTGGAACAA
ACTGTTCCAGCATTATTGGCACATTTACCACTAAACACCGGTTTTGAAGAATACAATCCA
ATTTTCGAATTGATCATGAAACTATACCAGGAAAATAGTCCCGTTATCACAAACGAAACC
CCAAGGATCATCGAGATTTTCAGTGCTGTGTTTACCAAAGAAAATGATAGAATTAAGTTA
GAAAAGGAGTCTACTCTAGGTAGGGAAGAGAATATGGAAAGATTAAAGCAATTCCAAACC
GAAGAGATGAAGCATAAGGTTATTGAATTATTGAAGTATCTAAACACTACTTACAACGGA
ATTGTTGCTCAAAATCCGGTTTTAGCTGCCGTCATTGCTTAG

Protein sequence predicted based on nucleotide sequence

MDQQFLSQLEQTLHAITSGVGLKEATKTLQTQFYTQPTTLPALIHILQNGSDDSLKQLAG
VEARKLVSKHWNAIDESTRASIKTSLLQTAFSEPKENVRHSNARVIASIGTEELDGNKWP
DLVPNLIQTASGEDVQTRQTAIFILFSLLEDFTSSLSGHIDDFLALFSQTINDPSSLEIR
SLSAQALNHVSALIEEQETINPVQAQKFAASIPSVVNVLDAVIKADDTMNAKLIFNCLND
FLLLDSQLTGNFIVDLIKLSLQIAVNSEIDEDVRVFALQFIISSLSYRKSKVSQSKLGPE
ITVAALKVACEEIDVDDELNNEDETGENEENTPSSSAIRLLAFASSELPPSQVASVIVEH
IPAMLQSANVFERRAILLAISVAVTGSPDYILSQFDKIIPATINGLKDTEPIVKLAALKC
IHQLTTDLQDEVAKFHEEYLPLIIDIIDSAKNIVIYNYATVALDGLLEFIAYDAIAKYLD
PLMNKLFYMLESNESSKLRCAVVSAIGSAAFAAGSAFIPYFKTSVHYLEKFIQNCSQIEG
MSEDDIELRANTFENISTMARAVRSDAFAEFAEPLVNSAYEAIKTDSARLRESGYAFIAN
LAKVYGENFAPFLKTILPEIFKTLELDEYQFNFDGDAEDLAAFADSANEEELQNKFTVNT
GISYEKEVASAALSELALGTKEHFLPYVEQSLKVLNEQVDESYGLRETALNTIWNVVKSV
LLASKVEPESYPKGIPASSYVNADVLAVIQAARETSMGNLSDEFETSMVITVMEDFANMI
KQFGAIIIMDNGDSSMLEALCMQVLSVLKGTHTCQTIDIEEDVPRDEELDASETEATLQD
VALEVLVSLSQALAGDFAKVFDNFRPVVFGLFQSKSKNKRSSAVGAASELALGMKEQNPF
VHEMLEALVIRLTSDKSLEVRGNAAYGVGLLCEYASMDISAVYEPVLKALYELLSAADQK
ALAAEDDEATREIIDRAYANASGCVARMALKNSALVPLEQTVPALLAHLPLNTGFEEYNP
IFELIMKLYQENSPVITNETPRIIEIFSAVFTKENDRIKLEKESTLGREENMERLKQFQT
EEMKHKVIELLKYLNTTYNGIVAQNPVLAAVIA*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3342
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable