Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
41997
Keyword:
None
Species:
Acidithiobacillus ferrooxidans ATCC 23270
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
AfCD00088982
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 669nts
Open reading frame: 51 to 719

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGGAGCGAT
TGCGCCGAACAGAAATATTCTCTGTCTGGTCGGATGAGGACCTTTCGCATTTATTTAAGG
GCGCACGTGTTCTGGAGATGAGTGTCGGGCAAATCCTTTTCCTTGAAGGCGATGCGGCTA
CCGCTTTCTATTTTCTTCTGGATGGACAAGTGAAGCTGGTGACGTCGGCTGCTGACGGCC
ACGAGAAAATAGTTGATATTTTGCAAGGCGGAGACCTTTTTGCCGAAGCTGTAGCTTTTT
TGGGATATCGTTATCCTGTGCAGGCAATAGCGGCCATGCCGAGCCGGGTTCTGGAAATTC
CCCTTACGATATTTCTCGTCACCCTACAGCAGCATCAGGAGCTGATGCTGCAAATGTTGG
GCAGACTGAGTCTGCGTTTACGTCATTTAATAATGGAGTTGCGACATCTTACTGTGGAAT
CCGCTGACCAACGGGTTGCAGGATATCTGCTGGAACTCTGCCCATCGGGAAATTCTCCCG
TGAGTTTGCTTCTCCCTGCCAAAAAAGCCATGATCGCGGCCCGACTAGGTTTAACCCCGG
AAACATTTTCTCGTGTGTTGAAGCGATTGCGAGAGGAGCATTTGATAGCTGTTGAAAAAC
TCAAGATATACATCCACCGCCCTTCTGAGTTACGGCAATGGGCGAAGGAAGACCGCTAAC
GCGACTTAATTAATTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAG

Protein sequence predicted based on nucleotide sequence

MERLRRTEIFSVWSDEDLSHLFKGARVLEMSVGQILFLEGDAATAFYFLLDGQVKLVTSA
ADGHEKIVDILQGGDLFAEAVAFLGYRYPVQAIAAMPSRVLEIPLTIFLVTLQQHQELML
QMLGRLSLRLRHLIMELRHLTVESADQRVAGYLLELCPSGNSPVSLLLPAKKAMIAARLG
LTPETFSRVLKRLREEHLIAVEKLKIYIHRPSELRQWAKEDR*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 669
Mutations:
None
Discrepancy:
g63a,L21L;c114t,L38L;
a126g,A42A;a234c,A78A;
c246t,A82A;t449c,V150A;
t468c,L156L;a504c,L168L;
g516a,K172K;g531c,A177A;
c597a,I199I;a598g,g600t,T200A;
t624c,I208I;c638a,A213E;
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
Find experimental protocols in 391567
Recommended expression in:
Not Applicable