CHC1 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH148667.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00008904
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 4962nts
Open reading frame: 1 to 4962

ATGAGTGACCTACCCATTGAATTTACCGAATTGGTCGATCTGATGTCCTTAGGAATTTCC
CCTCAATTCCTTGACTTCAGATCAACTACTTTCGAGAGTGACCACTTCGTCACTGTTAGA
GAAACAAAGGACGGTACAAACTCTGTTGCAATCGTGGATTTGGCCAAAGGCAATGAAGTG
ACAAGGAAGAATATGGGCGGTGATTCTGCTATCATGCATCCATCTCAGATGGTTATTTCC
GTTAGAGCAAATGGTACTATCGTGCAGATATTTAATTTGGAAACTAAGAGCAAGTTAAAG
TCTTTTACTTTAGATGAACCTGTTATTTTTTGGAGGTGGTTGAGTGAAACCACTTTGGGT
TTTGTTACCGCAAGATCCATACTGACCTCGAACGTCTTTGATGGTAACGTAAATGCTAAG
CCTCAATTGCTTACCTTGAGACACGCTAACTTGAACAATACCCAAATTATCAATTTTGTG
GCTAACAAAAACCTTGACTGGTTCGCAGTTGTTGGTATCTTACAAGAAAATGGCCGTATT
GCCGGTAGAATTCAATTATTCTCAAAACAACGTAACATCTCCCAAGCTATCGACGGTCAT
GTTGCTATCTTTACGAATATATTACTAGAGGGTAATGGCTCGACCCCGGTACAAGTTTTC
GTTACTGGTAACAGAAATGCCACCACCGGTGCTGGAGAATTAAGAATTATCGAGATTGAT
CACGATGCTTCATTGCCTTCTCAATATCAAAAGGAAACTACCGATATTTTCTTTCCACCA
GATGCAACGAATGATTTCCCAATCGCTGTTCAGGTGTCTGAGAAATACGGCATAATTTAC
CTATTAACCAAGTATGGCTTCATCCATTTGTACGAATTGGAAACCGGTACCAATTTATTC
GTTAATAGAATTACAGCAGAATCTGTTTTTACTGCAGCTCCGTACAATCACGAGAACGGT
ATTGCATGCATCAATAAAAAAGGTCAAGTTTTAGCAGTAGAGATTTCTACTTCTCAAATC
GTTCCATATATCTTGAATAAATTATCTAATGTGGCACTGGCCTTAATCGTTGCTACAAGA
GGTGGCTTACCAGGTGCAGATGATTTATTTCAAAAACAGTTTGAATCTTTATTGTTACAA
AACGATTATCAAAATGCAGCTAAAGTTGCAGCCTCTTCCACTTCTTTAAGGAACCAAAAC
ACCATCAACAGGTTGAAAAACATTCAAGCACCACCAGGCGCCATTTCTCCTATCCTACTT
TACTTTTCAACACTATTGGATAAAGGGAAATTGAATAAAGAGGAAACAATTGAATTGGCT
AGACCAGTTCTACAACAAGACAGAAAACAATTGTTCGAAAAATGGTTAAAGGAAGATAAA
CTAGAATGTTCAGAAGAACTAGGTGACATCGTTAAGCCTTTCGATACTACTTTAGCGCTA
GCTTGCTACTTAAGAGCAGGTGCCCATGCAAAAGTTATATCTTGTCTTGCCGAATTACAA
CAGTTTGAAAAAATTATTCCTTACTGCCAAAAGGTTGGATATCAACCTAACTTTTTAGTG
CTGATTTCTTCTTTGATTAGATCATCACCAGACAGAGCTTCTGAATTTGCCGTATCTCTA
TTACAAAATCCGGAAACTGCTTCTCAGATCGACATAGAAAAAATCGCAGATCTATTCTTT
TCCCAAAACCATATCCAACAAGGAACCTCTCTACTTCTAGACGCGTTAAAAGGAGATACC
CCAGACCAAGGTCACTTGCAAACTCGTGTTCTTGAAGTCAATCTACTACACGCACCACAA
GTTGCTGACGCCATATTGGGAAACAACATCTTTTCTCATTATGACAAACCAACTATTGCT
TCCTTATCCGAAAAGGCGGGACTATACCAGAGAGCTTTAGAAAATTACACAGACATCAAA
GATATCAAGAGATGCGTTGTCCACACCAATGCATTACCTATCGATTGGTTAGTCGGATAC
TTTGGTAAGTTGAACGTTGAACAATCTTTGGCGTGCTTAAAAGCTTTAATGGATAACAAT
ATTCAGGCTAACATCCAGACCGTAGTACAGGTGGCAACTAAATTCTCTGATTTGATCGGT
CCATCTACTTTGATTAAATTATTCGAAGATTATAATGCTACGGAAGGTTTATACTATTAC
TTAGCCTCTTTAGTCAACCTAACCGAAGATAAAGATGTGGTTTACAAATACATTGAAGCT
GCTGCTAAAATGAAACAATACAGAGAAATTGAAAGAATTGTGAAGGATAACAATGTCTAT
GATCCAGAAAGAGTCAAGAATTTCTTGAAGGATGCCAATCTAGAAGACCAGTTGCCTCTA
GTCATAGTTTGTGACCGTTTCGACTTTGTTCATGAAATGATTTTATATTTGTACAAATCA
CAGAACTTAAAATTTATTGAAACTTACGTGCAGCAGGTTAACCCTTCAAAGACTGCCCAA
GTTGTTGGCGCATTACTTGATATGGACTGTGATGAGGCATTTATTCAAAGTCTGCTACAA
TCAGTCTTAGGTCAAGTCCCTATTAATGAATTAACTACTGAGGTTGAAAAGAGAAATAGA
CTAAAAATATTGCTACCTTTCCTAGAACAAAGCTTAAGTCAAGGTATCCAGGATCAAGCT
GTTTACAATGCCTTAGCAAAAATTTATATTGATTCCAATAATTCTCCTGAAAAGTTTTTG
AAGGAAAACGACCAATATGACACCTTAGACGTTGGTCATTACTGCGAAAAAAGAGATCCC
TACCTTGCATATATTGCGTATGAAAAAGGTCAAAATGATGATGACTTGATTAGAATCACT
AATGAAAATAGCATGTACAAGTACCAGGCAAGATATTTGTTGGAACGTTCTGATCTCGAT
TTATGGAACAAAGTTTTGAATCAAGAAAATATTCACAGAAGACAATTAATTGATTCGGTC
ATCTCCGTTGGTATCCCTGAATTAACTGATCCTGAACCTGTTTCCTTAACTGTTCAAGCA
TTCATGACCAATGGATTAAAACTGGAACTAATTGAGTTGCTAGAAAAAATCATATTGGAA
CCTTCTCCTTTCAACGAAAATGTGGCTTTACAAGGGTTGCTATTATTGTCTGCTATTAAG
TATGAACCAACTAAGGTTTCAAGCTATATCGAAAAATTAGATAACTACGACGCCGATGAA
ATTGCTCCATTATGTATTGAACATGATTTGAAGGAGGAAGCTTTTGAAATCTATGACAAA
CATGAAATGTACGGTAAAGCTTTAAAGGTTTTAATCGAAGACATCATGTCTTTAGATAGA
GCTGCTTCTTATGCTGATAAAATCAATACTCCCGAATTGTGGTCTCAAATTGGTACGGCT
CAGTTAGATGGATTAAGAATCCCAGATGCTATTGAGTCTTATATTAAAGCAGAAGACCCA
TCAAATTACGAAAATGTAATTGATATAGCCGAACAAGCCGGTAAATACGAAGAATTAATT
CCATTTTTACTAATGGCTAGAAAGACTTTGAAAGAACCAAAGATCGACGGTGCTTTAATC
TTGGCCTATGCTGAACTAAACAAGATTCATGAAATTGAGAACTTGTTGGCAGGTTCCAAT
GTTGCAAACTTAGATCATGTTGGTGATAAATTATTCGAGAATAAAGAGTATAAAGCGGCC
AGGCTATGTTATTCTGCTGTTTCTAATTACTCCAAGCTTGCTTCTACCTTGGTTTACCTG
GGAGATTATCAGGCTGCTGTTGACACTGCTAGAAAGGCTTCAAACATTAAAGTTTGGAAA
CTGGTTAACGACGCTTGTATTGAAAAGAAAGAATTCAAGCTGGCTCAAATTTGTGGGTTG
AACTTGATCGTTCACGCTGAAGAATTAGATGAACTGGTGGAAAGGTATGAATCAAACGGC
TATTTCGAAGAACTGATTTCATTGTTTGAAGCTGGTTTGGGACTTGAAAGAGCTCATATG
GGCATGTTCACTGAATTGGCCATTTTGTATTCCAAATATGAACCAGACAAGACCTTCGAA
CACTTGAAGCTATTCTGGTCCAGAATAAATATTCCAAAGGTTATTAGAGCTGTCGAACAA
GCTCACCTCTGGTCGGAATTGGTCTTTTTATATGCTCACTATGATGAATGGGATAATGCT
GCTTTAACACTAATCGAAAAATCAACCAAAGACTTGGATCACGCCTATTTCAAAGAAGTT
GTTGTCAAAGTTTCTAACTTAGAAATCTATTACAAGGCCATTAATTTCTACGTCAAATTT
CACCCATCATTACTAGTTGATCTTTTGACCTCATTGACACCAAGATTAGATATTCCAAGA
ACTGTCAAAATTTTCTCGAAATCCGACAACTTGCCTTTAATCAAGCCTTTCTTGATCAAT
GTCTTACCAAAAAATAACTCAGTTGTCAACCAAGCCTACCACGATCTGATGATTGAAGAA
GAAGATTATAAAGCTTTGCAAGACGCTGTTGATTCTTATGACAAATTTGACCAATTGGGA
TTGGCATCTCGTTTGGAATCTCATAAGCTAATTTTTTTCAAGAAAATAGGAGCCCTGTTA
TACCGTAGAAATAAGAAGTGGGCAAAGAGTCTTTCAATCTTGAAGGAAGAAAAGCTATGG
AAGGACGCTATTGAGACGGCAGCAATCTCTCAGGATCCAAAGGTAGTGGAGGCTCTACTC
ACATATTTCGTTGAAACCGGTAACAGAGAAGGCTTTGTTGCTTTGCTATATGCTGCTTAC
AACTTAGTGAGAATTGAATTTGTTTTAGAAATATCATGGATGAATTCTTTGGAGGACTAC
ATTAAGCCATTTGAAATCTCCATAAAAAAAGAGCAGAATGATTCCATTAAGAAAATCACC
GAAGAACTCGCTAAAAAGTCCGGATCTAATGAAGAGCACAAAGATGGCCAACCTTTGATG
CTGATGAACAGCGCGATGAACGTTCAACCCACAGGATTTTAG

Protein sequence predicted based on nucleotide sequence

MSDLPIEFTELVDLMSLGISPQFLDFRSTTFESDHFVTVRETKDGTNSVAIVDLAKGNEV
TRKNMGGDSAIMHPSQMVISVRANGTIVQIFNLETKSKLKSFTLDEPVIFWRWLSETTLG
FVTARSILTSNVFDGNVNAKPQLLTLRHANLNNTQIINFVANKNLDWFAVVGILQENGRI
AGRIQLFSKQRNISQAIDGHVAIFTNILLEGNGSTPVQVFVTGNRNATTGAGELRIIEID
HDASLPSQYQKETTDIFFPPDATNDFPIAVQVSEKYGIIYLLTKYGFIHLYELETGTNLF
VNRITAESVFTAAPYNHENGIACINKKGQVLAVEISTSQIVPYILNKLSNVALALIVATR
GGLPGADDLFQKQFESLLLQNDYQNAAKVAASSTSLRNQNTINRLKNIQAPPGAISPILL
YFSTLLDKGKLNKEETIELARPVLQQDRKQLFEKWLKEDKLECSEELGDIVKPFDTTLAL
ACYLRAGAHAKVISCLAELQQFEKIIPYCQKVGYQPNFLVLISSLIRSSPDRASEFAVSL
LQNPETASQIDIEKIADLFFSQNHIQQGTSLLLDALKGDTPDQGHLQTRVLEVNLLHAPQ
VADAILGNNIFSHYDKPTIASLSEKAGLYQRALENYTDIKDIKRCVVHTNALPIDWLVGY
FGKLNVEQSLACLKALMDNNIQANIQTVVQVATKFSDLIGPSTLIKLFEDYNATEGLYYY
LASLVNLTEDKDVVYKYIEAAAKMKQYREIERIVKDNNVYDPERVKNFLKDANLEDQLPL
VIVCDRFDFVHEMILYLYKSQNLKFIETYVQQVNPSKTAQVVGALLDMDCDEAFIQSLLQ
SVLGQVPINELTTEVEKRNRLKILLPFLEQSLSQGIQDQAVYNALAKIYIDSNNSPEKFL
KENDQYDTLDVGHYCEKRDPYLAYIAYEKGQNDDDLIRITNENSMYKYQARYLLERSDLD
LWNKVLNQENIHRRQLIDSVISVGIPELTDPEPVSLTVQAFMTNGLKLELIELLEKIILE
PSPFNENVALQGLLLLSAIKYEPTKVSSYIEKLDNYDADEIAPLCIEHDLKEEAFEIYDK
HEMYGKALKVLIEDIMSLDRAASYADKINTPELWSQIGTAQLDGLRIPDAIESYIKAEDP
SNYENVIDIAEQAGKYEELIPFLLMARKTLKEPKIDGALILAYAELNKIHEIENLLAGSN
VANLDHVGDKLFENKEYKAARLCYSAVSNYSKLASTLVYLGDYQAAVDTARKASNIKVWK
LVNDACIEKKEFKLAQICGLNLIVHAEELDELVERYESNGYFEELISLFEAGLGLERAHM
GMFTELAILYSKYEPDKTFEHLKLFWSRINIPKVIRAVEQAHLWSELVFLYAHYDEWDNA
ALTLIEKSTKDLDHAYFKEVVVKVSNLEIYYKAINFYVKFHPSLLVDLLTSLTPRLDIPR
TVKIFSKSDNLPLIKPFLINVLPKNNSVVNQAYHDLMIEEEDYKALQDAVDSYDKFDQLG
LASRLESHKLIFFKKIGALLYRRNKKWAKSLSILKEEKLWKDAIETAAISQDPKVVEALL
TYFVETGNREGFVALLYAAYNLVRIEFVLEISWMNSLEDYIKPFEISIKKEQNDSIKKIT
EELAKKSGSNEEHKDGQPLMLMNSAMNVQPTGF*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 4962
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable