UFD4 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH148837.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00008906
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 4452nts
Open reading frame: 1 to 4452

ATGTCTGAAAATAATTCGCACAACCTTGATGAACATGAGTCCCATAGCGAAAACAGTGAT
TATATGATGGATACGCAGGTAGAAGATGACTATGATGAGGATGGCCATGTACAGGGTGAG
TACTCTTATTATCCTGATGAAGATGAAGATGAACATATGCTTTCTAGCGTCGGAAGTTTT
GAGGCAGATGATGGTGAAGATGACGATAACGATTACCATCATGAAGATGATTCTGGACTT
TTATATGGATATCATAGAACTCAGAATGGCAGTGACGAAGACAGAAATGAAGAAGAAGAT
GGACTTGAACGTTCTCACGATAATAATGAATTTGGCAGCAACCCCCTACATTTACCTGAC
ATTTTGGAAACATTCGCACAAAGACTAGAACAAAGAAGACAAACAAGTGAAGGACTTGGG
CAACACCCGGTTGGAAGAACACTACCCGAGATTTTATCGATGATTGGAGGAAGGATGGAG
AGGAGCGCAGAGAGTTCGGCAAGGAATGAGCGGATTTCTAAATTGATAGAGAATACTGGG
AATGCCTCCGAGGATCCTTATATTGCAATGGAGAGTTTAAAAGAACTTTCTGAAAACATA
TTAATGATGAATCAAATGGTTGTCGATAGAATTATACCGATGGAAACCTTAATAGGTAAT
ATAGCTGCCATACTCTCTGATAAAATTTTACGGGAAGAATTAGAATTACAAATGCAAGCT
TGTAGATGCATGTATAATCTTTTTGAGGTCTGCCCTGAATCTATTTCAATAGCTGTTGAT
GAACACGTTATACCAATTTTACAAGGAAAATTGGTAGAGATCAGTTACATTGACCTCGCA
GAACAAGTTTTAGAAACGGTGGAATATATTTCTAGAGTACATGGGAGAGACATTTTAAAA
ACGGGCCAATTATCAATCTACGTCCAATTCTTCGATTTTTTAACTATACATGCGCAGAGG
AAGGCTATCGCAATTGTTTCGAACGCCTGTAGCAGTATCCGAACGGATGACTTTAAGACC
ATTGTTGAAGTACTTCCAACGCTGAAGCCAATTTTCTCGAATGCGACAGACCAACCAATA
TTAACCAGGCTTGTAAATGCCATGTACGGTATTTGCGGGGCGTTGCATGGGGTTGACAAA
TTTGAGACTTTGTTTTCGTTGGATTTAATCGAAAGAATAGTTCAGCTAGTTTCTATTCAG
GATACCCCCTTGGAGAATAAACTGAAATGTTTGGATATTTTAACCGTATTAGCGATGAGT
AGTGATGTACTTTCAAGAGAACTGAGAGAGAAAACTGACATTGTCGACATGGCAACACGA
TCATTCCAGCATTATAGTAAAAGTCCTAACGCAGGGTTACATGAAACACTGATTTATGTC
CCAAACAGTTTATTGATTAGCATTTCTAGATTTATAGTTGTATTGTTTCCTCCCGAGGAT
GAAAGAATACTGTCAGCGGATAAATATACCGGAAATAGCGACCGTGGCGTAATTTCTAAC
CAGGAAAAGTTTGATTCCCTAGTTCAATGTCTAATTCCAATTCTCGTTGAAATTTATACA
AATGCTGCTGACTTTGACGTAAGAAGATACGTACTTATTGCTTTACTGAGGGTTGTATCA
TGCATAAATAATTCCACAGCAAAGGCAATCAATGATCAACTTATTAAGTTAATCGGATCT
ATCCTGGCCCAAAAAGAAACAGCGTCTAACGCTAATGGTACTTACTCATCAGAAGCTGGT
ACACTGTTGGTTGGTGGTCTCTCGTTGCTTGACTTAATTTGTAAAAAGTTTTCCGAACTG
TTCTTTCCTTCCATCAAAAGAGAGGGCATTTTTGATTTGGTTAAGGATTTGTCTGTGGAT
TTCAATAACATTGATTTAAAGGAAGACGGGAATGAAAATATTTCACTTTCTGACGAAGAA
GGGGATTTGCATAGCAGTATTGAGGAATGTGATGAGGGTGATGAAGAATATGATTACGAA
TTTACTGATATGGAAATTCCTGATTCAGTAAAACCAAAGAAAATTTCAATACACATTTTC
AGAACTCTATCTCTAGCTTATATTAAAAACAAGGGTGTGAACCTAGTTAATAGAGTACTT
TCTCAGATGAACGTTGAGCAAGAAGCAATAACAGAGGAGCTCCATCAAATCGAAGGCGTT
GTTTCTATTTTAGAAAATCCTTCCACTCCGGACAAAACTGAAGAGGATTGGAAGGGAATT
TGGTCTGTTTTAAAAAAATGTATTTTCCATGAAGATTTCGACGTGTCAGGTTTCGAATTT
ACTTCTACAGGGCTAGCTTCCTCCATAACTAAAAGAATTACATCCTCAACGGTATCCCAT
TTCATTCTTGCTAAATCATTTTTAGAGGTATTTGAGGATTGTATTGACAGATTTTTAGAA
ATCCTACAATCTGCTCTCACAAGGCTGGAGAATTTCTCTATAGTTGATTGCGGTTTACAC
GATGGTGGTGGTGTATCTTCACTGGCTAAAGAGATAAAAATTAAGTTGGTTTATGATGGC
GATGCAAGCAAAGATAATATTGGTACTGATTTATCATCTACTATCGTTTCGGTCCATTGC
ATAGCTTCTTTTACCTCACTTAATGAGTTTTTGAGACACAGAATGGTAAGAATGCGTTTT
TTGAATTCATTAATCCCAAACCTTACATCTTCCAGTACCGAAGCTGATAGGGAAGAAGAA
GAAAATTGCTTGGATCATATGAGAAAAAAGAACTTTGACTTTTTTTATGATAATGAAAAA
GTTGACATGGAGTCTACAGTATTTGGTGTGATATTTAATACATTCGTCAGGCGAAATCGT
GACTTAAAAACTTTATGGGATGATACACATACAATCAAATTTTGCAAAAGTTTAGAAGGT
AACAATAGAGAGAGTGAGGCAGCCGAGGAAGCTAATGAGGGGAAAAAGTTAAGAGATTTT
TATAAAAAAAGAGAATTCGCACAGGTTGATACTGGATCTTCAGCGGATATTCTGACATTG
CTGGATTTTCTACATAGCTGCGGTGTTAAAAGCGACAGTTTTATCAACTCAAAACTAAGC
GCTAAGCTCGCTAGACAACTAGATGAACCATTGGTAGTAGCAAGTGGAGCTTTGCCGGAT
TGGTCACTATTTTTGACCAGGAGATTCCCATTTTTGTTTCCGTTTGATACCAGGATGCTT
TTCCTACAATGTACTTCATTTGGTTACGGAAGATTGATTCAACTTTGGAAGAATAAGAGT
AAAGGCTCGAAAGATTTAAGGAATGACGAAGCTTTACAACAACTTGGGAGAATTACTAGG
CGTAAGCTGCGGATTTCAAGAAAAACAATATTCGCTACCGGTCTCAAGATTTTATCCAAG
TACGGAAGTAGCCCTGACGTACTGGAAATTGAATATCAAGAAGAAGCAGGAACAGGTTTA
GGACCGACTTTGGAATTTTACTCCGTAGTTTCCAAGTATTTTGCAAGAAAGTCGTTAAAT
ATGTGGCGTTGTAACTCTTATAGTTACAGAAGCGAAATGGATGTTGATACTACTGACGAT
TATATTACCACTTTATTGTTCCCAGAGCCCCTCAACCCGTTTTCCAATAATGAAAAAGTT
ATTGAACTTTTTGGATATTTGGGGACATTTGTTGCCAGATCGTTGCTTGATAATAGAATT
CTTGACTTTAGATTTAGCAAAGTCTTTTTTGAGTTATTGCACAGAATGTCTACGCCCAAT
GTGACGACAGTGCCGAGCGACGTTGAAACCTGTCTGTTAATGATCGAATTGGTAGATCCG
TTACTCGCAAAATCCCTTAAATACATAGTAGCGAATAAGGATGACAATATGACCCTAGAA
TCGTTGTCCTTGACATTTACCGTTCCTGGAAATGATGACATTGAGTTGATTCCGGGGGGT
TGTAATAAATCCTTGAACTCTTCTAATGTTGAAGAATATATCCATGGCGTTATCGACCAA
ATATTAGGTAAGGGCATTGAAAAACAGTTAAAAGCATTTATTGAAGGTTTTTCAAAGGTG
TTTTCCTATGAGAGGATGCTAATACTTTTTCCGGATGAATTAGTGGATATTTTCGGACGA
GTTGAGGAAGACTGGTCTATGGCAACTTTATACACAAACTTGAACGCTGAACATGGCTAT
ACAATGGATTCTTCAATCATTCATGATTTTATATCAATAATATCCGCGTTTGGTAAGCAT
GAAAGAAGATTATTTTTGCAATTTTTAACGGGATCTCCCAAGCTTCCAATTGGGGGATTT
AAAAGTTTGAACCCAAAGTTTACAGTTGTGTTAAAGCATGCTGAAGATGGCCTAACAGCA
GACGAATATCTACCAAGTGTAATGACATGTGCTAATTATTTGAAATTGCCGAAGTATACT
AGCAAAGATATTATGCGGTCTCGTCTTTGTCAAGCCATTGAAGAGGGTGCAGGAGCTTTT
CTACTTTCCTAG

Protein sequence predicted based on nucleotide sequence

MSENNSHNLDEHESHSENSDYMMDTQVEDDYDEDGHVQGEYSYYPDEDEDEHMLSSVGSF
EADDGEDDDNDYHHEDDSGLLYGYHRTQNGSDEDRNEEEDGLERSHDNNEFGSNPLHLPD
ILETFAQRLEQRRQTSEGLGQHPVGRTLPEILSMIGGRMERSAESSARNERISKLIENTG
NASEDPYIAMESLKELSENILMMNQMVVDRIIPMETLIGNIAAILSDKILREELELQMQA
CRCMYNLFEVCPESISIAVDEHVIPILQGKLVEISYIDLAEQVLETVEYISRVHGRDILK
TGQLSIYVQFFDFLTIHAQRKAIAIVSNACSSIRTDDFKTIVEVLPTLKPIFSNATDQPI
LTRLVNAMYGICGALHGVDKFETLFSLDLIERIVQLVSIQDTPLENKLKCLDILTVLAMS
SDVLSRELREKTDIVDMATRSFQHYSKSPNAGLHETLIYVPNSLLISISRFIVVLFPPED
ERILSADKYTGNSDRGVISNQEKFDSLVQCLIPILVEIYTNAADFDVRRYVLIALLRVVS
CINNSTAKAINDQLIKLIGSILAQKETASNANGTYSSEAGTLLVGGLSLLDLICKKFSEL
FFPSIKREGIFDLVKDLSVDFNNIDLKEDGNENISLSDEEGDLHSSIEECDEGDEEYDYE
FTDMEIPDSVKPKKISIHIFRTLSLAYIKNKGVNLVNRVLSQMNVEQEAITEELHQIEGV
VSILENPSTPDKTEEDWKGIWSVLKKCIFHEDFDVSGFEFTSTGLASSITKRITSSTVSH
FILAKSFLEVFEDCIDRFLEILQSALTRLENFSIVDCGLHDGGGVSSLAKEIKIKLVYDG
DASKDNIGTDLSSTIVSVHCIASFTSLNEFLRHRMVRMRFLNSLIPNLTSSSTEADREEE
ENCLDHMRKKNFDFFYDNEKVDMESTVFGVIFNTFVRRNRDLKTLWDDTHTIKFCKSLEG
NNRESEAAEEANEGKKLRDFYKKREFAQVDTGSSADILTLLDFLHSCGVKSDSFINSKLS
AKLARQLDEPLVVASGALPDWSLFLTRRFPFLFPFDTRMLFLQCTSFGYGRLIQLWKNKS
KGSKDLRNDEALQQLGRITRRKLRISRKTIFATGLKILSKYGSSPDVLEIEYQEEAGTGL
GPTLEFYSVVSKYFARKSLNMWRCNSYSYRSEMDVDTTDDYITTLLFPEPLNPFSNNEKV
IELFGYLGTFVARSLLDNRILDFRFSKVFFELLHRMSTPNVTTVPSDVETCLLMIELVDP
LLAKSLKYIVANKDDNMTLESLSLTFTVPGNDDIELIPGGCNKSLNSSNVEEYIHGVIDQ
ILGKGIEKQLKAFIEGFSKVFSYERMLILFPDELVDIFGRVEEDWSMATLYTNLNAEHGY
TMDSSIIHDFISIISAFGKHERRLFLQFLTGSPKLPIGGFKSLNPKFTVVLKHAEDGLTA
DEYLPSVMTCANYLKLPKYTSKDIMRSRLCQAIEEGAGAFLLS*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 4452
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable