PEP1 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH148887.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00008907
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 4740nts
Open reading frame: 1 to 4740

ATGATATTACTTCATTTTGTCTATTCTCTTTGGGCCTTACTTCTCATTCCTTTAACTAAT
GCCGAAGAATTCACCCCCAAAGTGACAAAGACTATCGCGCAAGATTCATTTGATATATTA
AGCTTTGATGATTCCAACACTTTAATTAGAAAACAAGACACCTCCGTTACTATAAGCTTT
GACGATGGTGAAACATGGGAAAAAGTTGAAGGCATTGAAGGCGAAATCACTTGGATATAC
ATCGACCCCTTCAATAGACATGACAGAGCCGTTGCAACGGCAATGAATGGGTCGTACCTT
TATATAACCAATGATCAAGGTAAGTCATGGGAGCGTATAACGCTACCTGACTCCGGAGAA
AGTATTTCACCTCGTGAATGCTATATAGAAACCCATCCCTTGAATAAGAACTATTTTCTT
GCAAAGTGCAACTATTGTGAGAAAACAGAAGTAAACAATGACAATGAAGAGAATTCGGGG
GACGAAGAGGGACAATTTGAAATATTTAATATTACACGTTGCACAGACAAGGTTTTTGCA
AGTAATGATGGTGGAAAATCCTTTTCTGAGATCAAGTCTTCTCTAGAAAGGAACGAAAAT
AGTCCTATCAGCATTTCTGATTGTGGCTTTGCCAAGACCAGCAAAGATTCTGACCTTGAA
AGTAGTGATACCTCAATAATCTGTCTTTTTCAAAATATGCAGCTTATTATGGATGAGTTT
AGTTCTCCTTACACCGAAAGTAAATTGGTCCTAACTACGGACTGGGGCAAATCACTAAAA
GAATTTGACCAATTTAAAGATAAGGTCGTCAATGGTTACAGGATATTGAAATCTCACATG
GTTGTCTTAACCCAGGGCGACAGATATAATGATATGTCTTCCATGGATGTGTGGGTATCA
AATGATCTGTCAAACTTTAAAATGGCTTACATGCCTACTCAGTTAAGGCATTCTATGCAA
GGAGAAATATATGAGGACGCTATGGGAAGAATTATCTTGCCCATGAGTAGGGAAAGAAGT
GATCAAGAGGAGGATAAGGGCATCGTGTCTGAAATTTTAATTTCCGACTCACAAGGGTTA
AAATTTTCCCCCATCCCATGGACCGCAAATGAGGTGTTTGGTTATATTAATTTTTATCAA
CCTACTTACTTGAAAGGAACGATGATTGCCTCACTTTACCCTCTATCTAGGCGTCGTAAC
CGTAAAGGAAAAGCCAAAGGAGTAAAGAGTAAGGGGGTAACCAAAATATCTGTTGATAAT
GGCCTCACATGGACAATGTTAAAAGTTGTTGATCCAGATAACGCAGACTCATTCGACTGT
GATATTACTGATTTTGAGAATTGTTCGCTTCAAAATATGTTTTACACACGGGAGGGTTCC
ACTCCAACCGCCGGAATTCTAATGACAACAGGTATTGTTGGCGATGGTAGTGTCTTCGAC
TGGGGAGATCAAAGAACCTTTATTTCTAGGGATGGTGGCTTAACATGGAAACTCGCCTTT
GATTTTCCTTGTTTATACGCTGTTGGTGATTACGGAAATGTTATTGTGGCTATACCGTAT
AATGCGGATGAAGACGACGATCCTCAATCCGAATTTTATTACTCTTTAGACCAAGGTAAA
ACTTGGACCGAATATCAGCTAGAAACTACTATCTACCCAAATGAAGTAATGAATACAACG
CCCGACGGATCTGGAGCTAAATTTATTCTAAATGGGTTTACTTTGGCGCATATGGATGGT
ACAACGAATTTCATCTATGCAATTGATTTTTCAACAGCCTTTAATGATAAGACATGCGAA
GAAAATGATTTCGAGGATTGGAATTTAGCTGAGGGGAAGTGTGTCAATGGAGTCAAGTAC
AAGATCAGAAGAAGAAAACAGGACGCTCAGTGCTTGGTGAAGAAAGTTTTTGAAGACTTA
CAATTATTTGAGACTGCTTGTGACAAGTGTACCGAGGCTGATTACGAATGCGCGTTTGAA
TTTGTTAGGGACGCGACCGGGAAATGCGTACCAGACTACAACCTAATCGTTCTCTCTGAC
GTATGTGATAAGACAAAGAAAAAAACTGTGCCTGTAAAACCATTGCAACTAGTTAAAGGT
GATAAATGTAAAAAACCAATGACAGTCAAATCAGTGGATATTTCGTGTGAGGGAGTTCCA
AAGAAGGGAACGAATGATAAAGAAATAGTGGTTACAGAAAACAAATTTGATTTCAAGATT
CAATTCTATCAATACTTTGACACAGTCACCGACGAATCCCTCCTCATGATCAATTCAAGA
GGAGAAGCTTATATATCTCATGATGGTGGACAAACAATAAAAAGGTTCGACAGTAATGGT
GAAACAATTATTGAAGTTGTGTTTAATCCATACTACAATTCTTCAGCTTATCTGTTTGGT
TCCAAAGGTAGCATTTTCTCTACCCATGATAGGGGATACTCTTTTATGACTGCTAAATTG
CCCGAGGCTAGGCAGTTAGGTATGCCATTAGACTTTAACGCTAAGGCACAGGATACATTT
ATCTATTATGGTGGTAAGAATTGTGAGTCAATCTTAAGTCCGGAATGTCATGCGGTAGCA
TACCTGACCAATGATGGGGGCGAAACGTTTACGGAAATGCTTGATAATGCAATTCATTGT
GAGTTTGCGGGCTCACTTTTCAAATATCCGTCAAATGAGGATATGGTTATGTGTCAAGTG
AAGGAAAAGTCTTCGCAGACAAGAAGCTTAGTTTCTTCTACTGATTTTTTCCAGGATGAT
AAAAATACCGTCTTTGAAAATATTATCGGCTACTTATCCACTGGTGGCTATATCATCGTT
GCTGTTCCTCATGAGAACAACGAATTGAGAGCATACGTAACTATCGATGGTACTGAGTTT
GCCGAGGCAAAATTCCCATATGATGAAGATGTTGGGAAGCAAGAGGCATTCACTATATTA
GAGTCTGAGAAAGGATCGATATTCTTACATTTAGCAACAAACTTAGTACCAGGACGCGAT
TTTGGCAATCTTTTGAAATCCAACTCAAATGGTACTTCTTTTGTCACGTTGGAGCATGCC
GTTAATAGAAACACATTCGGCTATGTTGACTTTGAAAAAATTCAAGGTCTCGAAGGCATT
ATTCTCACCAACATCGTTTCAAATAGTGACAAGGTCGCCGAGAATAAAGAAGACAAACAA
TTGAAGACGAAGATCACCTTTAATGAAGGTTCAGATTGGAACTTTTTGAAACCTCCGAAG
AGGGATTCAGAAGGAAAAAAGTTTTCTTGCAGCTCCAAATCACTGGATGAGTGTTCATTG
CACTTACATGGCTATACTGAACGTAAGGATATTAGAGATACATATTCTTCCGGTTCTGCA
TTAGGAATGATGTTCGGCGTTGGCAACGTTGGTCCTAACCTTTTACCATATAAAGAATGT
TCCACCTTCTTCACCACCGATGGTGGCGAAACGTGGGCTGAAGTTAAGAAGACTCCTCAC
CAATGGGAATACGGTGACCACGGTGGGATTTTAGTTTTAGTTCCTGAAAACTCAGAAACT
GATTCTATTTCCTATTCTACCGATTTTGGTAAAACATGGAAAGATTATAAATTCTGCGCT
GATAAGGTTTTAGTAAAGGATATAACCACTGTTCCCAGGGATTCTGCTTTGAGATTTTTG
CTGTTTGGAGAGGCAGCAGATATTGGAGGCAGCTCATTTAGAACGTACACAATTGATTTT
AGAAACATCTTCGAAAGACAATGTGATTTCGACATCACTGGTAAGGAAAGCGCAGATTAT
AAATACTCTCCTCTGGGTTCCAAAAGCAATTGCCTATTTGGTCACCAAACCGAGTTTTTA
CGTAAAACCGATGAAAATTGTTTTATTGGGAATATTCCACTTTCTGAATTTTCAAGAAAT
ATCAAAAACTGTTCTTGTACAAGACAAGATTTCGAGTGTGATTACAACTTTTACAAAGCT
AACGATGGTACTTGTAAATTAGTCAAAGGACTAAGCCCAGCAAATGCTGCAGACGTTTGT
AAAAAAGAGCCAGATTTAATCGAATATTTTGAATCGTCAGGCTACAGAAAGATCCCTCTA
TCAACCTGTGAGGGTGGCCTGAAATTGGATGCTCCCTCATCACCACATGCTTGCCCAGGA
AAAGAAAAAGAATTCAAGGAAAAGTACTCAGTAAGTGCCGGTCCCTTTGCATTTATTTTC
ATTTCAATTCTTTTAATAATTTTCTTTGCCGCATGGTTTGTATATGACAGAGGTATCAGA
AGAAATTGGGGATTTGCAAGGTTTGGAGAAATTAGGCTAGGTGACGATGGTTTAATAGAA
AACAATAATACTGACAGAGTTGTCAATAACATTGTGAAATCAGGATTTTACGTTTTCTCA
AATATCGGGTCTCTTTTACAGCACACAAAAACTAATATAGCGCATGCTATCTCCAAAATT
AGAGGAAGGTTTGGAAACAGAACAGGTCCAAGCTACTCATCCCTGATCCATGATCAATTT
TTGGATGAAGCAGATGACCTGCTTGCTGGCCACGATGAAGACGCCAATGACTTATCCAGT
TTCATGGATCAGGGTAGTAATTTTGAAATCGAAGAAGATGATGTTCCAACACTTGAAGAA
GAGCATACATCATATACAGATCAACCTACGACCACCGATGTTCCAGATACATTACCAGAA
GGAAATGAGGAAAACATCGACAGGCCTGATTCTACAGCGCCATCTAACGAAAACCAGTAG

Protein sequence predicted based on nucleotide sequence

MILLHFVYSLWALLLIPLTNAEEFTPKVTKTIAQDSFDILSFDDSNTLIRKQDTSVTISF
DDGETWEKVEGIEGEITWIYIDPFNRHDRAVATAMNGSYLYITNDQGKSWERITLPDSGE
SISPRECYIETHPLNKNYFLAKCNYCEKTEVNNDNEENSGDEEGQFEIFNITRCTDKVFA
SNDGGKSFSEIKSSLERNENSPISISDCGFAKTSKDSDLESSDTSIICLFQNMQLIMDEF
SSPYTESKLVLTTDWGKSLKEFDQFKDKVVNGYRILKSHMVVLTQGDRYNDMSSMDVWVS
NDLSNFKMAYMPTQLRHSMQGEIYEDAMGRIILPMSRERSDQEEDKGIVSEILISDSQGL
KFSPIPWTANEVFGYINFYQPTYLKGTMIASLYPLSRRRNRKGKAKGVKSKGVTKISVDN
GLTWTMLKVVDPDNADSFDCDITDFENCSLQNMFYTREGSTPTAGILMTTGIVGDGSVFD
WGDQRTFISRDGGLTWKLAFDFPCLYAVGDYGNVIVAIPYNADEDDDPQSEFYYSLDQGK
TWTEYQLETTIYPNEVMNTTPDGSGAKFILNGFTLAHMDGTTNFIYAIDFSTAFNDKTCE
ENDFEDWNLAEGKCVNGVKYKIRRRKQDAQCLVKKVFEDLQLFETACDKCTEADYECAFE
FVRDATGKCVPDYNLIVLSDVCDKTKKKTVPVKPLQLVKGDKCKKPMTVKSVDISCEGVP
KKGTNDKEIVVTENKFDFKIQFYQYFDTVTDESLLMINSRGEAYISHDGGQTIKRFDSNG
ETIIEVVFNPYYNSSAYLFGSKGSIFSTHDRGYSFMTAKLPEARQLGMPLDFNAKAQDTF
IYYGGKNCESILSPECHAVAYLTNDGGETFTEMLDNAIHCEFAGSLFKYPSNEDMVMCQV
KEKSSQTRSLVSSTDFFQDDKNTVFENIIGYLSTGGYIIVAVPHENNELRAYVTIDGTEF
AEAKFPYDEDVGKQEAFTILESEKGSIFLHLATNLVPGRDFGNLLKSNSNGTSFVTLEHA
VNRNTFGYVDFEKIQGLEGIILTNIVSNSDKVAENKEDKQLKTKITFNEGSDWNFLKPPK
RDSEGKKFSCSSKSLDECSLHLHGYTERKDIRDTYSSGSALGMMFGVGNVGPNLLPYKEC
STFFTTDGGETWAEVKKTPHQWEYGDHGGILVLVPENSETDSISYSTDFGKTWKDYKFCA
DKVLVKDITTVPRDSALRFLLFGEAADIGGSSFRTYTIDFRNIFERQCDFDITGKESADY
KYSPLGSKSNCLFGHQTEFLRKTDENCFIGNIPLSEFSRNIKNCSCTRQDFECDYNFYKA
NDGTCKLVKGLSPANAADVCKKEPDLIEYFESSGYRKIPLSTCEGGLKLDAPSSPHACPG
KEKEFKEKYSVSAGPFAFIFISILLIIFFAAWFVYDRGIRRNWGFARFGEIRLGDDGLIE
NNNTDRVVNNIVKSGFYVFSNIGSLLQHTKTNIAHAISKIRGRFGNRTGPSYSSLIHDQF
LDEADDLLAGHDEDANDLSSFMDQGSNFEIEEDDVPTLEEEHTSYTDQPTTTDVPDTLPE
GNEENIDRPDSTAPSNENQ*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 4740
Mutations:
None
Discrepancy:
g4267t,G1423W
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable