Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
1038.1.08_GO.34674
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pVP16
Format: 
FUSION
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Center for Eukaryotic Structural Genomics
ScCD00089189
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 2340nts
Open reading frame: 51 to 2390

GGATCGACGAAAACCTGTACCTCACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCAAGGACAGTGTAGACTGCCCAAGCATTCTACCCACCGACCGCACTTCAG
TGCTTTCCGAGACTAGCACTTTGGTCGGATCGAGTTCGCATGTATATTCGAGGCATGCTC
CTATGAACAGTTATCATAACAGCATGAACAGCAACATATACCACAGCCCCAAAGCCAGTA
GCCCTTTGGTGAGCTATAAGACATCTTCGCCGGTGTTATTGAAGAGGGCGACCGCTCCTG
TCCTGCCAAGCTTCAAGCCCAAGGAACAGCGATATAATAAGCCGCAGGGTTGTTCGCTAA
TTACTGCTGTAGAACTGGGCAAGATTATTGAAACCTTACCAGATGAAAAGGTGCTCTTAC
TTGATGTTAGGCCGTTTACCGAACATGCCAAATCCATTATCACAAACTCCATTCATGTAT
GTTTGCCTTCCACGCTGCTGAGGAGGAAAAATTTCACCTTTTCCAAACTCTTGGACAACT
TAACTCCTAGCGAGCAAAGCGTGTTGAAATCTAAACTGGCCATCGATAACCTTCGGATTA
TCATTTATGATAGCACCGCAAATCAAACGGAAAGTTCTGTTTCTTTACCTTGTTATGGAA
TAGCGTCCAAGCTAATAGAGTTTGATACTAATGTTAAAAAAACCGTGTCTATACTAATGT
GTGGTTTTCCTCAATTCAAAATTCTATTTCCAGACCATATAAACACCAACACATTTAACT
CAGATTGCATTAGTTCGGCTGAACCTAAAAGCCCCAAGACAAACCTTATGAACAGTCTAC
ACAACACAGCGCCGCATATGACGGCGACAACACCACTATCGTCTCCGCAAATGAACCTTA
AGTTGAAAGTTCCTGACGATAGTAGGTCAGATCATTCAAACTTTTCCAGTTCTCCCTCTC
CGCGTAACGTCCTATCAGATTCTCCCATGTCTTCTTCTTCCCCCATATCTGCCTTATTTA
AGTTCCAGCTGCCTGCACCACAAACAAACATAAACCAAATGTTCAAATTTTCGCAAAATG
AAGAAATAATGGGTTTAGAGACGTATTTGAGTGCAGTCAACATCAAAGAAGAACATGAAA
GATGGTATAATAACGATTCTGCCAAAAAATCTTTACAAAACTTCCAATTCCCAAAGAACC
AAAATTCTCTAGAAAAGGACACTAATAAAGACAAACTAGGGTTTCAGATCAGATACGAAA
ACCTGAGCAAAAATTACGAAAAGGAGGTCATTGACTCCGTTATACCTGAATGGTTCCAGC
ATCTGATGTCAATACCCAAGATCGAGTTGGTTTCTCAGTTTCAAAAATTAGATTTTTTGG
AAAAAAGGCGCTTGAACCATTCTGTCTCTTTCAGGAAGAAGGAAAACTCGTTTATACTTG
AAAAGCCATCATCATATCCTGAACAGTTAACATCAACTTCTTCTTCAACAATCATGCCTC
CAAAATTTCCTGACGTGAACAAAGTACAAAAGAGGTCGCATTCTCAGCCAATCTTCACTC
AATACAGTAAATATAAATCGATGCTCAGCCTTGAATCAGATTCCGATTCGGAGTCAGATG
ACGTTATAATATCATCAGGCGTTGAATTAGGAGCCAAGAACAGGTACAAGGATATTTTTC
CCTACGAGCATTCGAGAGTGATTCTAAAAAAAGGTTTGCAATCATCAAAAGGTATCAAGC
ATTCACATTCTACTTCCGATGGTGGCATCCTCGACAATTATATCAACGCAAACTATTTGT
CTCTGCCAAGGTTTTCTGTAGAGCAAAATTCGTCATTTCAAACAACTACAACCACAACAA
GAAGAGTGCGCTATATAGCGACGCAAGCTCCAATGCCCTCCACTGTACATGACTTTTATA
CATGCATATTGAATAATGGCGTTCCGTTGGTTCTCTCATTGACAAATGATTTCGAAAATG
GCATTGAGAAATGTTATCGATATTGGCAAGAAGGTAATTATAACGGTATTCACGTTAAAT
TGTTAGAAAAAAAAATATTAAAGATGCCTAGCACTACTAGCATGAGGAAAAATACTATGG
GAACACAAAATAGTAGTTTATATTCAGCTGGTGTTCAGGGAAATAGTAGTAACTATAGTA
CTGATAATGATAATGATAATGACAACAATAATAATAACAATAACAATAGTAATATTGCTG
TTACTGCTGCTGCTTGTGATGATGATGATGATGATGATGATGACGCAATTCTCATAAGAA
AAATTCTGCTAACATATCATGATCAAGAGAAACCTTATGAATTGTTACAGATTCAGGTGA
AAAATTGGCCTGATTTGGGCACTTTACTCAATCCTATCTCAATCTTACAAGCAATCAATG
TCAAGAATCACATAATAGATACACTATTTGCCAGAAATTA

Protein sequence predicted based on nucleotide sequence

ENLYFQSKDSVDCPSILPTDRTSVLSETSTLVGSSSHVYSRHAPMNSYHNSMNSNIYHSP
KASSPLVSYKTSSPVLLKRATAPVLPSFKPKEQRYNKPQGCSLITAVELGKIIETLPDEK
VLLLDVRPFTEHAKSIITNSIHVCLPSTLLRRKNFTFSKLLDNLTPSEQSVLKSKLAIDN
LRIIIYDSTANQTESSVSLPCYGIASKLIEFDTNVKKTVSILMCGFPQFKILFPDHINTN
TFNSDCISSAEPKSPKTNLMNSLHNTAPHMTATTPLSSPQMNLKLKVPDDSRSDHSNFSS
SPSPRNVLSDSPMSSSSPISALFKFQLPAPQTNINQMFKFSQNEEIMGLETYLSAVNIKE
EHERWYNNDSAKKSLQNFQFPKNQNSLEKDTNKDKLGFQIRYENLSKNYEKEVIDSVIPE
WFQHLMSIPKIELVSQFQKLDFLEKRRLNHSVSFRKKENSFILEKPSSYPEQLTSTSSST
IMPPKFPDVNKVQKRSHSQPIFTQYSKYKSMLSLESDSDSESDDVIISSGVELGAKNRYK
DIFPYEHSRVILKKGLQSSKGIKHSHSTSDGGILDNYINANYLSLPRFSVEQNSSFQTTT
TTTRRVRYIATQAPMPSTVHDFYTCILNNGVPLVLSLTNDFENGIEKCYRYWQEGNYNGI
HVKLLEKKILKMPSTTSMRKNTMGTQNSSLYSAGVQGNSSNYSTDNDNDNDNNNNNNNNS
NIAVTAAACDDDDDDDDDAILIRKILLTYHDQEKPYELLQIQVKNWPDLGTLLNPISILQ

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 2390
Mutations:
None
Discrepancy:
g2338c,g2339a,G780Q;
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
ProteinExpressed: Tested_Not_Found
Find experimental protocols in GO.34674
Recommended expression in:
Not Applicable