Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
976.1.14_GO.34577
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pVP16
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Center for Eukaryotic Structural Genomics
MmCD00089196
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1260nts
Open reading frame: 51 to 1310

GGATCGACGAAAACCTGTACCTCACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCGCGTTCCTGTGTCAGCGCGACAGCTACGCACGGGAGTTCACCACCACCG
TGGTCTCCTGTAGTCCCGCCGAGCTGCAGACGGACGCGAGCGGCGGCAAGAAAGAAGTGT
TGAGCGGATTCCATGTGGTTCTGGAAGACACGCTGCTTTTCCCCGAGGGCGGGGGACAGC
CTGATGACCGTGGTACCATCAATGACATCTCTGTGCTGAGGGTGACCCGTCGTGGGGCCC
AGGCCGATCACTTCACGGAGTCACCTCTGTCCCCTGGGAGTCAAGTCCAGGTCCGGGTGG
ACTGGGAGCGGAGGTTTGACCACATGCAGCAGCATTCAGGGCAACATCTCATCACCGCGG
TTGCTGACCTTCTCTTCGGGCTGAAGACAACGTCATGGGAGTTAGGGAGACTCCGGAGTG
TGATTGAGTTGGACAGCCCTTCTGTGACTGCTGAGCAGGTGGCTGCTATCGAGCAGAGTG
TCAATCAGAAAATCAGAGACCGGCTGCCTGTGAGTGTTCGAGAGCTGAGCCTGGATGACC
CTGAGGTGGAGCAGGTGAGGGGTCGGGGTTTGCCCGATGATCATGCTGGGCCCATTCGAG
TTGTTACCATCGAAGGTGTAGACTCCAACATGTGCTGTGGGACGCACGTGAGCAATCTCA
GTGACCTTCAGGTCATTAAGATTCTGGGGACTGAGAAAGGGAAAAAGAACAAAAGCAACC
TGATATTTCTGGCTGGGAATCGGGTACTGAAGTGGATGGAGCGAAGTCATGGAAGTGAAA
AGGCCTTGACCTCACTACTTAAGTGTGGCGTTGAGGATCATGTGGAAGCAGTAAAAAAGC
TCCAGAATGCCACCAAGCTCCTGCAGAAGAACAACCTGAACCTCCTTAGAGACCTGGCTG
TGCACACTGCCCACAGCCTCAGGAGCAGCCCAGCCTGGGGTGGTGTGGTCACACTACACA
GGAAAGAGGGTGATTCTGAATTCATGAATATCATTGCTAATGAGATTGGATCGGAGGAGA
CCCTCCTGTTCTTAACTGTGGGGGATGAGAAGGGTGCTGGGCTCTTCTTACTGGCAGGCC
CGGCAGAGGCTGTGGAAACCCTGGGGCCCAGGGTGGCTGAAGTCTTGGAAGGCAAAGGAG
CAGGGAAGAAGGGCCGCTTCCAGGGCAAAGCCACCAAGATGAGCCGCCGGGCAGAGGCGC
AGGCGCTTCTGCAGGACTATGTCAGCACACAGAGTGCTGAGGAGTGATAGGACCCAGCTT
TCTTGTACAAAGTGGTGATGGCGCGCCTGTAGGACGTCGA

Protein sequence predicted based on nucleotide sequence

ENLYFQSAFLCQRDSYAREFTTTVVSCSPAELQTDASGGKKEVLSGFHVVLEDTLLFPEG
GGQPDDRGTINDISVLRVTRRGAQADHFTESPLSPGSQVQVRVDWERRFDHMQQHSGQHL
ITAVADLLFGLKTTSWELGRLRSVIELDSPSVTAEQVAAIEQSVNQKIRDRLPVSVRELS
LDDPEVEQVRGRGLPDDHAGPIRVVTIEGVDSNMCCGTHVSNLSDLQVIKILGTEKGKKN
KSNLIFLAGNRVLKWMERSHGSEKALTSLLKCGVEDHVEAVKKLQNATKLLQKNNLNLLR
DLAVHTAHSLRSSPAWGGVVTLHRKEGDSEFMNIIANEIGSEETLLFLTVGDEKGAGLFL
LAGPAEAVETLGPRVAEVLEGKGAGKKGRFQGKATKMSRRAEAQALLQDYVSTQSAEE**

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1310
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in GO.34577
Recommended expression in:
Not Applicable